Cyclosporin A inhibits the replication of diverse coronaviruses
Adriaan H de Wilde, Jessika C Zevenhoven-Dobbe, Yvonne van der Meer, Volker Thiel, Krishna Narayanan, Shinji Makino, Eric J Snijder, Martijn J van Hemert, Adriaan H de Wilde, Jessika C Zevenhoven-Dobbe, Yvonne van der Meer, Volker Thiel, Krishna Narayanan, Shinji Makino, Eric J Snijder, Martijn J van Hemert
Abstract
Low micromolar, non-cytotoxic concentrations of cyclosporin A (CsA) strongly affected the replication of severe acute respiratory syndrome coronavirus (SARS-CoV), human coronavirus 229E and mouse hepatitis virus in cell culture, as was evident from the strong inhibition of GFP reporter gene expression and a reduction of up to 4 logs in progeny titres. Upon high-multiplicity infection, CsA treatment rendered SARS-CoV RNA and protein synthesis almost undetectable, suggesting an early block in replication. siRNA-mediated knockdown of the expression of the prominent CsA targets cyclophilin A and B did not affect SARS-CoV replication, suggesting either that these specific cyclophilin family members are dispensable or that the reduced expression levels suffice to support replication.
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Source: PubMed