Twins born after transplantation of ovarian cortical tissue and oocyte vitrification
María Sánchez-Serrano, Juana Crespo, Vicente Mirabet, Ana C Cobo, María-José Escribá, Carlos Simón, Antonio Pellicer, María Sánchez-Serrano, Juana Crespo, Vicente Mirabet, Ana C Cobo, María-José Escribá, Carlos Simón, Antonio Pellicer
Abstract
Objective: To present a combination of ovarian tissue and oocyte cryopreservation as an effective strategy for achieving pregnancy in a breast cancer patient.
Design: Case report.
Setting: Tertiary care university-affiliated hospital, tissue bank, and infertility clinic.
Patient(s): A 36-year-old patient diagnosed with atypical medullar breast cancer and negative for estrogen, P, and HER2 receptors underwent ovarian tissue cryopreservation before receiving chemotherapy and radiotherapy.
Intervention(s): Laparoscopic ovarian cortex extraction, ovarian tissue cryopreservation, ovarian tissue thawing and transplantation, controlled ovarian stimulation (COS), oocyte retrieval, vitrification and IVF, and embryo culture and replacement.
Main outcome measure(s): Resumption of spontaneous ovarian function after transplantation, response to COS, oocyte vitrification, IVF, pregnancy, and delivery.
Result(s): Menses occurred 63 days after transplantation. Sixteen mature oocytes were obtained in four COS procedures. All vitrified oocytes survived warming, and 77.7% were fertilized. Two day 3 embryos were replaced, and two healthy boys were born at 34 weeks.
Conclusion(s): Ovarian tissue cryopreservation and grafting preserves fertility. Simultaneous oocyte vitrification increases the success of assisted reproductive technology in poor-prognosis patients and avoids the consequences of the short lifespan of the transplanted tissue.
Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Source: PubMed