Effects of 12-Week Exercise Program on Enzyme Activity of Serum Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 in Female Patients with Postmenopausal Osteoporosis: A Randomized Control Study

Tamara Filipović, Kristina Gopčević, Sanja Dimitrijević, Marija Hrković, Ana Backović, Milica Lazović, Tamara Filipović, Kristina Gopčević, Sanja Dimitrijević, Marija Hrković, Ana Backović, Milica Lazović

Abstract

Background: Osteoporosis is a disease characterized by decreased bone density and destruction of bone microarchitecture. Indicators for altered bone homeostasis are changes in the serum level of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs). The purpose of the current study was to evaluate the effect of a 12-week exercise program on enzyme activity of serum MMP-9 and TIMP-1 in postmenopausal osteoporotic patients. Materials and methods. Participants were randomized in two groups: exercise (EG) (N = 37) and control (CG) (N = 37) and control (CG) (.

Results: Significant differences between pretreatment and posttreatment enzyme activities of serum MMP-9 (p=0.009), TIMP-1 (p=0.009), TIMP-1 (p=0.009), TIMP-1 (.

Conclusion: Our results suggest that a 12-week exercise program has an influence on enzyme activity of serum MMP-9, revealing a possible role of MMPs in initiating training-specific adaptation. Although measurements of circulating MMP-9 and TIMP-1 allowed us to detect effects of exercise, as of today, they have no real role in the diagnosis of osteoporosis and/or follow-up of osteoporotic patient's response to treatment. MMP-9 might be used as an important prognostic marker for the evaluation of patient's response to exercise. Larger-randomized controlled studies need to be performed to expand this area of knowledge. This trial is registered with trial registration number: NCT03816449).

Conflict of interest statement

The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

Copyright © 2020 Tamara Filipović et al.

Figures

Figure 1
Figure 1
Participants flowchart.
Figure 2
Figure 2
MMP-9, TIMP-1, and MMP-9/TIMP-1ratio followed over time in EG and CG.
Figure 3
Figure 3
Serum activity of MMP-9 in the EG obtained by gelatin zymography. Representative protein bands of the MMP-9 protein are shown (a) before and (b) after the 12-week exercise program.

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