Hair Regeneration Treatment Using Adipose-Derived Stem Cell Conditioned Medium: Follow-up With Trichograms

Hirotaro Fukuoka, Hirotaka Suga, Hirotaro Fukuoka, Hirotaka Suga

Abstract

Objective: Adipose-derived stem cells secrete various growth factors that promote hair growth. This study examined the effects of adipose-derived stem cell-conditioned medium on alopecia.

Methods: Adipose-derived stem cell-conditioned medium was intradermally injected in 22 patients (11 men and 11 women) with alopecia. Patients received treatment every 3 to 5 weeks for a total of 6 sessions. Hair numbers were counted using trichograms before and after treatment. A half-side comparison study was also performed in 10 patients (8 men and 2 women).

Results: Hair numbers were significantly increased after treatment in both male (including those without finasteride administration) and female patients. In the half-side comparison study, the increase in hair numbers was significantly higher on the treatment side than on the placebo side.

Conclusion: Treatment using adipose-derived stem cell-conditioned medium appears highly effective for alopecia and may represent a new therapy for hair regeneration.

Keywords: adipose-derived stem cells; alopecia; conditioned medium; half-side comparison study; trichograms.

Figures

Figure 1
Figure 1
TrichoScope images of a 24-year-old male patient. (A) Before treatment. The number of hairs in the circle is 126. (B) After treatment (7 months after initial treatment). The number of hairs in the circle is 141.
Figure 2
Figure 2
Changes in the number of hairs before and after treatment in male and female patients. *P < .01.
Figure 3
Figure 3
Changes in the number of hairs before and after treatment in male patients with or without finasteride administration. *P < .05.
Figure 4
Figure 4
A 49-year-old male patient. (A) Before treatment. (B) During treatment (4 months after initial treatment). (C) After treatment (10 months after initial treatment). (D) Final follow-up (2 years and 1 month after initial treatment).
Figure 5
Figure 5
A 44-year-old female patient. (A) Before treatment. (B) After treatment (7 months after initial treatment). (C) Final follow-up (1 year and 10 months after initial treatment).
Figure 6
Figure 6
Changes in the number of hairs before and after treatment in the half-side comparison study. *P < .05 and †P < .01.
Figure 7
Figure 7
Increases in the number of hairs in the half-side comparison study. *P < .01.

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