Elevated endothelial progenitor cells during painful sickle cell crisis
Rachel T van Beem, Erfan Nur, Jaap Jan Zwaginga, Precious P Landburg, Eduard J van Beers, Ashley J Duits, Dees P Brandjes, Ingrid Lommerse, Hetty C de Boer, C Ellen van der Schoot, John-John B Schnog, Bart J Biemond, CURAMA Study Group, Rachel T van Beem, Erfan Nur, Jaap Jan Zwaginga, Precious P Landburg, Eduard J van Beers, Ashley J Duits, Dees P Brandjes, Ingrid Lommerse, Hetty C de Boer, C Ellen van der Schoot, John-John B Schnog, Bart J Biemond, CURAMA Study Group
Abstract
Objective: Circulating endothelial progenitor cells (EPCs) counts were determined in patients with sickle cell disease (SCD) to elucidate their role in SCD-related ischemia-induced angiogenesis and reendothelialization.
Materials and methods: Circulating EPC counts (KDR(+)/CD34(+)/Cd45(dim) cells) and their relation to serum levels of EPC mobilizing growth factors erythropoietin, vascular endothelial growth factor, and interleukin-8 were investigated in SCD patients during asymptomatic state (n=66) and painful crisis (n=36) and compared to healthy controls (n=13).
Results: EPC counts were comparable between controls (0; range, 0-1.1 cells/mL) and patients (0; range, 0-0 cells/mL) in asymptomatic state, but were significantly higher during painful crisis (41.7; range, 0-186 cells/mL; p<0.05). Also in a paired analysis of 12 patients who were included both during asymptomatic state and painful crisis, EPC counts increased significantly during painful crisis (from 0 [range, 0-0] to 26 [range, 0-149 cell/mL; p<0.05). EPC counts were not related to any of the measured growth factors.
Conclusion: The higher EPC counts during painful crisis might indicate a role for EPC mobilization in reendothelialization. As a relationship of EPCs with the established mobilizing growth factors, measured in this study was not observed, the mechanism of EPC mobilization in SCD remains to be elucidated.
Source: PubMed