Effects of topical oxygen therapy on ischemic wound healing

Congqiang Rao, Liling Xiao, Hongwei Liu, Shenghong Li, Jinqiang Lu, Jiangxuan Li, Shixing Gu, Congqiang Rao, Liling Xiao, Hongwei Liu, Shenghong Li, Jinqiang Lu, Jiangxuan Li, Shixing Gu

Abstract

[Purpose] This study evaluated the effects of topical oxygen therapy on the hind limb wounds of rats under ischemic conditions. [Subjects and Methods] Twelve injured rats were treated with topical oxygen on skin wounds located on the hind limb and compared with twelve injured control rats. Indexes including gross morphology of the wound, wound healing time, wound healing rate, and histological and immunohistochemical staining of sections of wound tissue were examined at different time points after intervention. [Results] The wound healing time was shorter in the topical oxygen therapy group than the control group. The wound healing rate and granulation tissue formation in the topical oxygen therapy group showed significant improvement on days 3, 7, and 14. Through van Gieson staining, the accumulation of collagen fiber in the topical oxygen therapy group was found to have improved when compared with the control group on day 7. Through semiquantitative immunohistochemical staining, many more new vessels were found in the topical oxygen therapy group compared with the model control group on day 7. [Conclusion] The results of the experiment showed that topical oxygen therapy improved ischemic wound healing.

Keywords: Animal model; Topical oxygen therapy; Wound healing.

Figures

Fig. 1.
Fig. 1.
A rat with the topical oxygen device on a limb The device supplied 100% oxygen at 1 atm of pressure
Fig. 2.
Fig. 2.
Typical macroscopic results showing that the wounds of the oxygen group healed faster than those of the control group
Fig. 3.
Fig. 3.
HE staining showed that the granulation tissues grew better in the TOT group than the model control group. On days 7–10, the inflammatory cells decreased in the TOT group; however, there were still large numbers of inflammatory cells in the control group
Fig. 4.
Fig. 4.
Collagen fibers in the wounds. On day 7, there were many more collagen fibers in the TOT group
Fig. 5.
Fig. 5.
The expression of CD31 showed that on day 7, there were many more new vessels in the TOT group than the model control group; however, on days 10-14, there were no significant differences between the two groups

References

    1. Bitto A, Minutoli L, Altavilla D, et al. : Simvastatin enhances VEGF production and ameliorates impaired wound healing in experimental diabetes. Pharmacol Res, 2008, 57: 159–169.
    1. Jeon JK, Park SK, Lee JH: Effects of high voltage pulsed current stimulation with a visible contraction intensity on expression of TGF-β1 and synthesis of type I collagen in wound-induced white rats. J Phys Ther Sci, 2015, 27: 1485–1490.
    1. Tandara AA, Mustoe TA: Oxygen in wound healing—more than a nutrient. World J Surg, 2004, 28: 294–300.
    1. Sen CK: Wound healing essentials: let there be oxygen. Wound Repair Regen, 2009, 17: 1–18.
    1. Leaper D: Perfusion, oxygenation and warming. Int Wound J, 2007, 4: 4–8.
    1. Black J: Tissue oxygen perfusion and pressure ulcer healing. Plast Surg Nurs, 2000, 20: 10–14.
    1. Chaudhuri V, Potts BR, Karasek MA: Mechanisms of microvascular wound repair I. Role of mitosis, oxygen tension, and I-kappa B. In Vitro Cell Dev Biol Anim, 2006, 42: 308–313.
    1. Hunt TK, Ellison EC, Sen CK: Oxygen: at the foundation of wound healing—introduction. World J Surg, 2004, 28: 291–293.
    1. Yan D, Shan J, Ze Y, et al. : The effects of combined hyperbaric oxygen therapy on patients with post-stroke depression. J Phys Ther Sci, 2015, 27: 1295–1297.
    1. Gordillo GM, Roy S, Khanna S, et al. : Topical oxygen therapy induces vascular endothelial growth factor expression and improves closure of clinically presented chronic wounds. Clin Exp Pharmacol Physiol, 2008, 35: 957–964.
    1. Rodriguez PG, Felix FN, Woodley DT, et al. : The role of oxygen in wound healing: a review of the literature. Dermatol Surg, 2008, 34: 1159–1169.
    1. Sibbald RG, Woo KY, Queen D: Wound bed preparation and oxygen balance—a new component? Int Wound J, 2007, 4: 9–17.
    1. Tracey AK, Alcott CJ, Schleining JA, et al. : The effects of topical oxygen therapy on equine distal limb dermal wound healing. Can Vet J, 2014, 55: 1146–1152.
    1. Wu L, Xia YP, Roth SI, et al. : Transforming growth factor-beta1 fails to stimulate wound healing and impairs its signal transduction in an aged ischemic ulcer model: importance of oxygen and age. Am J Pathol, 1999, 154: 301–309.
    1. Jonsson K, Jensen JA, Goodson WH, 3rd, et al. : Tissue oxygenation, anemia, and perfusion in relation to wound healing in surgical patients. Ann Surg, 1991, 214: 605–613.
    1. LaVan FB, Hunt TK: Oxygen and wound healing. Clin Plast Surg, 1990, 17: 463–472.
    1. Hopf HW, Hunt TK, West JM, et al. : Wound tissue oxygen tension predicts the risk of wound infection in surgical patients. Arch Surg, 1997, 132: 997–1004, discussion 1005.
    1. Hohn DC, MacKay RD, Halliday B, et al. : Effect of O2 tension on microbicidal function of leukocytes in wounds and in vitro. Surg Forum, 1976, 27: 18–20.
    1. Sen CK, Khanna S, Babior BM, et al. : Oxidant-induced vascular endothelial growth factor expression in human keratinocytes and cutaneous wound healing. J Biol Chem, 2002, 277: 33284–33290.
    1. Hickey MM, Simon MC: Regulation of angiogenesis by hypoxia and hypoxia-inducible factors. Curr Top Dev Biol, 2006, 76: 217–257.
    1. Chen CH, Wang JH, Lu SN, et al. : Characteristics of adefovir resistance in patients with or without lamivudine-resistant hepatitis B virus treated with adefovir: a 4-year experience. Liver Int, 2011, 31: 206–214.
    1. Patel V, Chivukula IV, Roy S, et al. : Oxygen: from the benefits of inducing VEGF expression to managing the risk of hyperbaric stress. Antioxid Redox Signal, 2005, 7: 1377–1387.
    1. Loo AE, Halliwell B: Effects of hydrogen peroxide in a keratinocyte-fibroblast co-culture model of wound healing. Biochem Biophys Res Commun, 2012, 423: 253–258.
    1. O’Toole EA, Marinkovich MP, Peavey CL, et al. : Hypoxia increases human keratinocyte motility on connective tissue. J Clin Invest, 1997, 100: 2881–2891.

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