Correlation Study of Isotype Switching and Neutralizing Capacity to the COVID-19 Variants Strain
19 oktober 2021 uppdaterad av: Wu Jiang, Centers for Disease Control and Prevention, China
Study on the Correlation of Isotype Switching, Neutralizing Antibody Titer and Cross Immunity Against COVID-19 Variants After Primary and Booster Vaccination
The level of neutralizing antibody was detected by using the constructed pseudovirus covid-19 variant at baseline , 1 month , 6 months and 12 months after primary vaccination, within 15 days after booster immunization at 12 months .
and the ability of IgG subclasses to neutralize and cross immunization of variant strains was analyzed.
Studieöversikt
Status
Aktiv, inte rekryterande
Betingelser
Detaljerad beskrivning
In 150 individuals who received primary and booster COVID-19 inactivated vaccine, the neutralization ability of antibodies against covid-19 mutant after primary and booster immunization was evaluated by constructing pseudotyped SARS-CoV-2 variants.
The contribution and influence of IgG subclasses on neutralization capacity were assessed by Elisa assay.
All participants were recruited aged between 18 and 59 years old.
Every participant was familiarized with the aim of the study and asked to sign an informed consent agreement and was required blood sampling at the day 0 of first dose vaccine and 1 month, 6 months after 2nd dose of primary immunization.
All participants had received 1 dose of booster immunization at 12 months after fully primary vaccination.
Then 150 people were divided into four groups, Blood samples were taken on days 0,3,7,10 and 14 to detect neutralizing antibodies and IgG subclasses separately.
every sample was detected SARS-CoV-2 variants strain Alpha, Beta and Delta neutralizing antibody, and RBD-IgG1, IgG2, IgG3, and IgG4.
every individual was conducted a detailed analysis correlation of isotype switching, neutralizing antibody titer and cross immunity against COVID-19 variants after primary and booster vaccination.
Studietyp
Observationell
Inskrivning (Faktisk)
150
Kontakter och platser
Det här avsnittet innehåller kontaktuppgifter för dem som genomför studien och information om var denna studie genomförs.
Studieorter
-
-
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Beijing, Kina, 100013
- Center for Disease Control and Prevention
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Deltagandekriterier
Forskare letar efter personer som passar en viss beskrivning, så kallade behörighetskriterier. Några exempel på dessa kriterier är en persons allmänna hälsotillstånd eller tidigare behandlingar.
Urvalskriterier
Åldrar som är berättigade till studier
18 år till 59 år (Vuxen)
Tar emot friska volontärer
Ja
Kön som är behöriga för studier
Allt
Testmetod
Sannolikhetsprov
Studera befolkning
The healthy people aged between 18 and 59, and have not been infected with COVID-19, have not been vaccinated with COVID-19 vaccine, and agree to collect blood samples.
Beskrivning
Inclusion Criteria:
- Between the ages of 18 and 59
- Healthy
- No vaccination with COVID-19 before become participant
- Agreed to take a blood sample
Exclusion Criteria:
- who cannot take the COVID-19 vaccine due to some personal reasons
- history of SARS-CoV, SARS-CoV-2, or Middle East respiratory syndrome infection
- high-risk epidemiology history within 14 days before enrolment (eg, travel or residence history in communities with case reports, or contact history with someone infected with SARS-CoV-2)
- axillary temperature of more than 37·0℃
- history of allergy to any vaccine component.
Studieplan
Det här avsnittet ger detaljer om studieplanen, inklusive hur studien är utformad och vad studien mäter.
Hur är studien utformad?
Designdetaljer
Vad mäter studien?
Primära resultatmått
Resultatmått |
Åtgärdsbeskrivning |
Tidsram |
|---|---|---|
|
Neutralizing capabilities to variant COVID-19 after primary and booster vaccination
Tidsram: Baseline，After fully vaccinated 1 and 6 months，Within 15 days after booster immunization at 12 months
|
The titers of neutralizing antibodies against live SARS-CoV-2 and Pseudotyped SARS-CoV-2 Variants strain Alpha, Beta and Delta at baseline, after fully vaccinated 1 and 6 months and Within 15 days after booster immunization at 12 months. The neutralizing antibodies was defined as a change from seronegative at baseline to seropositive or a four-fold titer increase. The positive cutoff of the titer for neutralizing antibodies was 1/30. |
Baseline，After fully vaccinated 1 and 6 months，Within 15 days after booster immunization at 12 months
|
|
Titer of Subclass of IgG after primary and booster vaccination
Tidsram: Baseline，After fully vaccinated 1 and 6 months，Within 15 days after booster immunization at an interval of 12 months
|
The titers of Subclass RBD-specific IgG1, IgG2,IgG3 and IgG4 at baseline, after fully vaccinated 1 and 6 months and Within 15 days after booster immunization at an interval of 12 months.
The positive for IgG1, IgG2,IgG3 and IgG4 antibodies are defined as the sample titer value ≥1/20.
|
Baseline，After fully vaccinated 1 and 6 months，Within 15 days after booster immunization at an interval of 12 months
|
Sekundära resultatmått
Resultatmått |
Åtgärdsbeskrivning |
Tidsram |
|---|---|---|
|
Titer of subclass of IgG at different points in time after booster vaccination
Tidsram: 0,3,7,10,14 days
|
Titer of subclass of IgG at 0,3,7,10,14 days after booster vaccination.
The positive for IgG1, IgG2,IgG3 and IgG4 antibodies are defined as the sample titer value ≥1/20.
|
0,3,7,10,14 days
|
Samarbetspartners och utredare
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Studieavstämningsdatum
Dessa datum spårar framstegen för inlämningar av studieposter och sammanfattande resultat till ClinicalTrials.gov. Studieposter och rapporterade resultat granskas av National Library of Medicine (NLM) för att säkerställa att de uppfyller specifika kvalitetskontrollstandarder innan de publiceras på den offentliga webbplatsen.
Studera stora datum
Studiestart (Faktisk)
20 juli 2020
Primärt slutförande (Faktisk)
10 oktober 2021
Avslutad studie (Förväntat)
30 oktober 2021
Studieregistreringsdatum
Först inskickad
19 oktober 2021
Först inskickad som uppfyllde QC-kriterierna
19 oktober 2021
Första postat (Faktisk)
21 oktober 2021
Uppdateringar av studier
Senaste uppdatering publicerad (Faktisk)
21 oktober 2021
Senaste inskickade uppdateringen som uppfyllde QC-kriterierna
19 oktober 2021
Senast verifierad
1 oktober 2021
Mer information
Termer relaterade till denna studie
Ytterligare relevanta MeSH-villkor
Andra studie-ID-nummer
- BJCDCWJ202102
Läkemedels- och apparatinformation, studiedokument
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