Evaluation of Prolidase in Obstructive Sleep Apnea Syndrome
2018年6月19日 更新者:Havva Sayhan、Sakarya University
Evaluation of Prolidase as an Oxidative Stress Marker in Obstructive Sleep Apnea Syndrome
Obstructive sleep apnea (OSA) is a highly prevalent breathing disorder in sleep.
We have aimed to evaluate the relationship between OSA and prolidase activity, the oxidative stress index (OSI), total antioxidative capacity (TAC), and total oxidative capacity (TOC) and the relationship between carotid intima media thickness (CIMT).
研究概览
地位
未知
详细说明
This study was approved by the local ethics committee in accordance with the Helsinki Declaration.
Written informed consent was received from the OSAS Subjects patients and control subjects before enrolment in the study.
The patient and control cohorts were recruited from the Pulmonary Medicine Department, Medical Faculty, Yuzuncu Yıl University.
Blood samples were analysed at the Biochemistry Laboratory of Harran University Medical Faculty TAC and TOS levels were measured by using an automated measurement method.
TAC measurement method involves the production of a potent biological hydroxyl radical.
Ferrous ion solution is mixed with hydrogen peroxide.
Thus, it is possible to measure the anti-oxidative effect of the sample against the potent free radical reactions initiated by the production of the hydroxyl radical.
TOS method is based on the oxidation of ferrous ion to ferric ion in the presence of various oxidant species in acidic medium and the measurement of the ferric ion by xylenol orange.
mmol Trolox equivalent (equiv)/L, mmol H2O2 /L and mg/dL, respectively.
研究类型
观察性的
注册 (预期的)
74
联系人和位置
本节提供了进行研究的人员的详细联系信息,以及有关进行该研究的地点的信息。
学习联系方式
- 姓名:Havva Sayhan
- 电话号码:+905056621021
- 邮箱:hsayhan@gmail.com
研究联系人备份
- 姓名:hulya gunbatar, asso. prof.
- 电话号码:+905065118827
- 邮箱:hulyagunbatar@gmail.com
学习地点
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-
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Sakarya、火鸡、54100
- 招聘中
- Sakarya University Research and Training hospital
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接触:
- Havva Sayhan, Assis Prof
- 电话号码:+905056621021
- 邮箱:hsayhan@gmail.com
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接触:
- müge yılmaz
-
-
Merkez
-
Sakarya、Merkez、火鸡、54100
- 招聘中
- Sakarya University Research and Training hospital
-
接触:
- Havva Sayhan, Assis Prof
- 电话号码:+905056621021
- 邮箱:hsayhan@gmail.com
-
接触:
- hulya gunbatar, assosiate. prof.
- 电话号码:+905065118827
- 邮箱:hulyagunbatar@gmail.com
-
-
参与标准
研究人员寻找符合特定描述的人,称为资格标准。这些标准的一些例子是一个人的一般健康状况或先前的治疗。
资格标准
适合学习的年龄
18年 至 65年 (成人、年长者)
接受健康志愿者
不
有资格学习的性别
全部
取样方法
非概率样本
研究人群
Patient (male/female: 43/31) were diagnosed with OSA after night polysomnography (PSG)
描述
Inclusion Criteria:
- Patients were diagnosed with OSA after night polysomnography (PSG).
- Patients were enrolled in the study following receipt of their written informed consent.
Exclusion Criteria:
- Patients had ischaemic cardiovascular diseases,
- Patients had chronic obstructive pulmonary diseases,
- Patients had ischaemic cerebral diseases,
- Patients had chronic inflammatory diseases,
- Patients had chronic and acute systemic infections.
学习计划
本节提供研究计划的详细信息,包括研究的设计方式和研究的衡量标准。
研究是如何设计的?
设计细节
队列和干预
团体/队列 |
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Group OSAS
We included 56 with OSAS [13 subjects 23.2% mild, 19 subjects 33.9% moderate, 24 subjects 42.8% severe
|
control group
simple snoring
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研究衡量的是什么?
主要结果指标
结果测量 |
措施说明 |
大体时间 |
---|---|---|
Prolidase activity
大体时间:1 month
|
Prolidase activity was determined by a photometric method based on the measurement of the proline levels produced by prolidase (26).
Plasma samples (100ml) were blended with 100 ml of serum physiological.
A total of 25 ml of the mixture was preincubated with 75ml of the preincubation solution (50 mmol/l Tris HCl buffer pH 7.0 containing 1mmol/l glutathion, 50mmol/l MnCl2) at 37˚C for 30min.
|
1 month
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合作者和调查者
在这里您可以找到参与这项研究的人员和组织。
调查人员
- 学习椅:Havva Sayhan、Sakarya University Research and Training hospital
出版物和有用的链接
负责输入研究信息的人员自愿提供这些出版物。这些可能与研究有关。
研究记录日期
这些日期跟踪向 ClinicalTrials.gov 提交研究记录和摘要结果的进度。研究记录和报告的结果由国家医学图书馆 (NLM) 审查,以确保它们在发布到公共网站之前符合特定的质量控制标准。
研究主要日期
学习开始 (预期的)
2018年7月1日
初级完成 (预期的)
2018年9月1日
研究完成 (预期的)
2018年12月1日
研究注册日期
首次提交
2018年6月7日
首先提交符合 QC 标准的
2018年6月19日
首次发布 (实际的)
2018年6月20日
研究记录更新
最后更新发布 (实际的)
2018年6月20日
上次提交的符合 QC 标准的更新
2018年6月19日
最后验证
2018年6月1日
更多信息
与本研究相关的术语
其他研究编号
- OSAS
计划个人参与者数据 (IPD)
计划共享个人参与者数据 (IPD)?
未定
药物和器械信息、研究文件
研究美国 FDA 监管的药品
不
研究美国 FDA 监管的设备产品
不
此信息直接从 clinicaltrials.gov 网站检索,没有任何更改。如果您有任何更改、删除或更新研究详细信息的请求,请联系 register@clinicaltrials.gov. clinicaltrials.gov 上实施更改,我们的网站上也会自动更新.