Differential EBI2 Expression Upon Allelic Status of the rs9557195 Polymorphism
研究概览
地位
条件
详细说明
EBI2 is a risk gene for inflammatory bowel diseases (rs9557195). EBI2 is a G protein coupled receptors expressed on the immune cells. EBI2 directs migration and activity of immune cells.
Patients of the Swiss IBD cohort study have been genotyped for the allelic status of EBI2. The investigators will recruit patients with Crohn's disease and ulcerative colitis, assess epidemiological and clinical parameters (including clinical activity of IBD) draw blood, isolate lymphocytes, DNA and RNA.
The investigators will test the influence of rs9557195 genotype (TT or CC allel), inflammatory activity and current treatment (infliximab vs. vedolizumab) on expression and activity of EBI2 on blood lymphocytes (by FACS using an EBI2-antibody), mRNA expression (by qPCR) of EBI2 and UBAC2 (located on the opposite DNA strand of EBI2) and activity of lymphocytes on a migration assay.
研究类型
注册 (预期的)
联系人和位置
学习地点
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Zurich、瑞士、8091
- 招聘中
- University Hospital Zürich
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接触：
- Rene Roth, MD
- 电话号码：++41 44 255 1111
- 邮箱：rene.roth@usz.ch
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参与标准
资格标准
适合学习的年龄
接受健康志愿者
有资格学习的性别
取样方法
研究人群
we will recruit a mixed population (patients can be in more than one group)
- up to 100 healthy volunteers
- up to 30 IBD patients with the rs9557195-CC IBD risk genotype in clinical remission
- up to 30 IBD patients with the rs9557195-CT IBD risk genotype in clinical remission
- up to 30 IBD patients with the rs9557195-TT IBD low risk genotype in clinical remission
- up to 50 IBD patients of any genotype during a flare
- up to 50 IBD patients of any genotype treated with vedolizumab
- up to 50 IBD patients of any genotype treated with a TNF-inhibitor (infliximab, adalimumab, golimumab, certolizumab)
描述
Inclusion Criteria:
- Patient with IBD OR healthy volunteer
- No major uncontrolled medical/ surgical/ psychiatric condition requiring ongoing management besides IBD in the respective study groups. Well controlled conditions (i.e. medically controlled arterial hypertension, occupational asthma) may be present
Exclusion Criteria: none
学习计划
研究是如何设计的？
设计细节
- 观测模型：队列
- 时间观点：横截面
队列和干预
团体/队列 |
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IBD patients and healthy volunteers
We will recruit IBD patients and healthy volunteers
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研究衡量的是什么？
主要结果指标
结果测量 |
措施说明 |
大体时间 |
|---|---|---|
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EBI2 expression - dependent on rs9557195 allele status (FACS)
大体时间：at time of inclusion into the study
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EBI2 levels in PBMCs will be determined by FACS, and compared according to allele status of rs9557195
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at time of inclusion into the study
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次要结果测量
结果测量 |
措施说明 |
大体时间 |
|---|---|---|
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EBI2 expression - dependent on rs9557195 allele status (RT-PCR)
大体时间：at time of inclusion into the study
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EBI2 levels in PBMCs will be determined by RT-PCR, and compared according to EBI2 levels in PBMCs will be determined by RT-PCR, and compared according to allele status of rs9557195
|
at time of inclusion into the study
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UBAC2 expression - dependent on rs9557195 allele status (RT-PCR)
大体时间：at time of inclusion into the study
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UBAC2 levels in PBMCs will be determined by RT-PCR, and compared according to 2 levels in PBMCs will be determined by RT-PCR, and compared according to allele status of rs9557195
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at time of inclusion into the study
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Motility of PBMCs dependent on rs9557195 allele status (Boyden chamber)
大体时间：at time of inclusion into the study
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motility of PBMCs will be assessed using the Boyden chamber and compared according to allele status of rs9557195
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at time of inclusion into the study
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EBI2-expression (FACS) in individuals treated with infliximab vs. vedolizumab
大体时间：at time of inclusion into the study
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We will compare EBI2 expression as determined by FACS in individuals with infliximab and vedolizumab treatment
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at time of inclusion into the study
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EBI2-expression (FACS) according to gut inflammation
大体时间：at time of inclusion into the study
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We will compare EBI2 expression (determined by FACS) in IBD patients with severe disease, in remission and in healthy volunteers
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at time of inclusion into the study
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合作者和调查者
研究记录日期
研究主要日期
学习开始 (实际的)
初级完成 (预期的)
研究完成 (预期的)
研究注册日期
首次提交
首先提交符合 QC 标准的
首次发布 (实际的)
研究记录更新
最后更新发布 (实际的)
上次提交的符合 QC 标准的更新
最后验证
更多信息
与本研究相关的术语
其他研究编号
- EBI2-Study
计划个人参与者数据 (IPD)
计划共享个人参与者数据 (IPD)？
IPD 计划说明
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研究美国 FDA 监管的设备产品
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