Gene Expression Profiles in Chronic Periodontitis

Gene Expression Profiles in Chronic Periodontitis: A Gene Network-based Microarray Analysis

Current concepts of the causes of periodontitis implicate a bacterial infection as the primary cause of the disease. Several bacterial species residing in a biofilm on tooth surfaces are closely associated with periodontitis. Chronic periodontitis (CP) is initiated and sustained by bacterial plaque, but host defense mechanisms play an integral role in its pathogenesis.

Gene expression profiling is a powerful means of generating comprehensive genome-level data sets on diseases such as cancer, asthma, rheumatoid disorders and periodontitis, and provides significant insight information for these diseases. Gene expression profiling may provide an evidence for involving genes in the pathogenesis of CP and generate further information other than clinical signs and symptoms of CP.

The aim of this study was to identify gene-expression patterns of patients with chronic periodontitis by whole-transcriptome gene-expression analyses.

Study Overview

• Status: Completed
• Conditions: Chronic Periodontitis
• Intervention / Treatment: Genetic: Genom-based whole-genome transcriptomic

• Study Type: Observational
• Enrollment (Actual): 46

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years and older (Adult, Older Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

• Sampling Method: Probability Sample

Study Population

The patientswere recruited from those patients seeking dental treatment in Kocaeli University, Faculty of Dentistry Periodontology Clinics.

Description

Inclusion Criteria:

1. CP patients had at least 20 teeth exhibiting >30% of measured sites with >5mm clinical attachment loss.
2. Had bleeding on probing (BOP) at >50% of the proximal sites.

Exclusion Criteria:

1. Had any known systemic diseases that can influence the periodontal status, oral diseases other than chronic periodontitis and ongoing orthodontic therapy.
2. History of hepatitis, HIV infection, or diabetes; requirement for antibiotic prophylaxis, pregnancy or lactation; and long-term use of anti-inflammatory drugs.
3. Should be free of systemic diseases
4. Individuals had to be older than 18 years of age.

Study Plan

How is the study designed?

Number of groups / cohorts

2

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
Patients with Chronic Periodontitis; All subjects were free of systemic diseases and to be included, individuals had to be older than 18 years of age. Chronic periodontitis was diagnosed according to American Academy of Periodontology 1999 Consensus Report.	Genetic: Genom-based whole-genome transcriptomic; Gene expression profiling is a powerful means of generating comprehensive genome-level data sets.
Control Patients; Control subjects had no radiographic evidence of bone loss or any sign of past and present periodontitis. All subjects were free of systemic diseases and to be included, individuals had to be older than 18 years of age.	Genetic: Genom-based whole-genome transcriptomic; Gene expression profiling is a powerful means of generating comprehensive genome-level data sets.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
mRNA quantification	2 months

Secondary Outcome Measures

Outcome Measure	Time Frame
Gen networking map	2 months

Collaborators and Investigators

• Sponsor: Kocaeli University
• Investigators: Principal Investigator: Esra Guzeldemir-Akcakanat, DDS, PhD, Chair and Associate Professor of Dept. of Periodontology

Publications and helpful links

General Publications

• Armitage GC. Development of a classification system for periodontal diseases and conditions. Ann Periodontol. 1999 Dec;4(1):1-6. doi: 10.1902/annals.1999.4.1.1.
• Demmer RT, Behle JH, Wolf DL, Handfield M, Kebschull M, Celenti R, Pavlidis P, Papapanou PN. Transcriptomes in healthy and diseased gingival tissues. J Periodontol. 2008 Nov;79(11):2112-24. doi: 10.1902/jop.2008.080139.
• Savli H, Sunnetci D, Cine N, Gluzman DF, Zavelevich MP, Sklyarenko LM, Nadgornaya VA, Koval SV. Gene expression profiling of B-CLL in Ukrainian patients in post-Chernobyl period. Exp Oncol. 2012;34(1):57-63.

Study record dates

Study Major Dates

• Study Start (Actual): February 1, 2013
• Primary Completion (Actual): August 1, 2015
• Study Completion (Actual): May 1, 2017

Study Registration Dates

• First Submitted: June 3, 2015
• First Submitted That Met QC Criteria: June 5, 2015
• First Posted (Estimate): June 10, 2015

Study Record Updates

• Last Update Posted (Actual): February 12, 2018
• Last Update Submitted That Met QC Criteria: February 9, 2018
• Last Verified: February 1, 2018

More Information

Terms related to this study

• Keywords: genetics; inflammation; chronic periodontitis; genes; gingiva; microarray analysis
• Additional Relevant MeSH Terms: Stomatognathic Diseases; Periodontal Diseases; Mouth Diseases; Periodontitis; Chronic Periodontitis
• Other Study ID Numbers: KOU KAEK 2013 / 15

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO