- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT04124354
Influence of Exercise on the Gut Microbiome of Overweight and Obese Adults With Prediabetes
Purpose: The investigators propose a 20-participant randomized 2-arm parallel trial with a delayed-intervention control examining how 8 weeks of moderate-intensity walking exercise alters the gut microbiome, short chain fatty acid (SCFA)-producing taxa, and the cardiometabolic profile and body weight of individuals who are overweight or obese and have prediabetes (PreD).
Aim 1: Examine and compare exercise-related shifts in the gut microbiome of individuals with PreD.
Aim 2: Examine and compare exercise-related changes in SCFA-producing taxa.
Exploratory Aim: Examine what percentage of the exercise-related changes observed in participants' gut microbiome and SCFA-producing taxa mediate changes in their cardiometabolic profile and body weight.
Study Overview
Detailed Description
In the U.S, 91.8 million adults have PreD. Preventing progression of PreD to type 2 diabetes (T2D) is vital. Odds of cardiovascular disease are higher among those with T2D, with $327 billion/year spent treating T2D. Major scientific organizations have thus called for studies of novel intermediates connecting health behaviors to pre-clinical cardiometabolic disease (CMD; e.g., PreD) to lower future clinical disease risk. Physical activity (PA) is key modifiable determinant of good health. PA recommendation adherence is associated with a 25-35% dose-dependent reduction in all-cause mortality, with as little as 75 min/week of regular moderate-intensity PA beneficial. In fact, an intervention within the Diabetes Prevention Program emphasizing PA participation at recommended levels was as successful as Metformin in preventing incident T2D-likely partially due to a 5kg-reduction in body weight during this trial. Yet, PA's impact on disease risk reduction is not explained entirely by CMD risk factors or weight loss, with mechanistic pathways still unclear and crucial to examine. The gut microbiota and microbiome have been posited as mechanistic intermediates linking PA to attenuated CMD development. However, there is no known research which has examined exercise-related changes in the human gut microbiome or SCFA-producing taxa in a population with a pre-clinical CMD such as PreD and how these changes mediate changes in CMD indices and body weight.
The proposed project is therefore innovative for a least two reasons. First, as stated, the investigators know of no study in individuals with PreD which examined how a moderate-intensity walking program may modify the gut microbiome and SCFA-producing taxa. Second, the investigators will use formal mediation analyses to examine the degree to which exercise-related changes in the gut microbiome and SCFA-producing taxa explain changes observed in CMD indices and body weight. These exploratory analyses will allow for a deeper interpretation of the physiological mechanisms by which exercise may improve health and inform future trial construction. Indeed, this study will provide critical preliminary evidence for a larger NIH R01-funded trial submitted to the NIDDK, NHLBI, or NCI, with the NIH committed to funding innovative scientific proposals involving the microbiome. The investigators will use this trial's observations to determine: 1) which taxa within the gut microbiome are most impacted by moderate-intensity walking; 2) the intra- and inter-person variability of gut microbiota changes due to moderate-intensity walking; and 3) whether the fixed frequency, intensity, and duration of the walking implemented results in meaningful changes. Observations in relation to these points will inform the design and implementation of future interventions to reduce pre-clinical disease states like PreD in a mechanistically-informed manner.
Study Type
Enrollment (Actual)
Phase
- Not Applicable
Contacts and Locations
Study Locations
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Minnesota
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Minneapolis, Minnesota, United States, 55455
- Epidemiology Clinical Research Center
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Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Genders Eligible for Study
Description
Inclusion Criteria:
- Classified as overweight or obese with BMI 25.0-39.9 kg/m2
- Diagnosis of prediabetes as classified by a fasted blood glucose of 100 - 125 mg/dL, 2-hour oral glucose tolerance test of 140 - 199 mg/dL, or HbA1C level of 5.7% - 6.5%
- Currently engaged in < 150 minutes/week of physical activity-confirmed via the Modifiable Activity Questionnaire
Exclusion Criteria:
- Individuals with contraindications to exercise participation as assessed by the Physical Activity Readiness Questionnaire
- Self-reported physical/mental disabilities or gastrointestinal conditions
- Antibiotic usage within the last 45 days
Study Plan
How is the study designed?
Design Details
- Primary Purpose: BASIC_SCIENCE
- Allocation: RANDOMIZED
- Interventional Model: PARALLEL
- Masking: NONE
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
---|---|
EXPERIMENTAL: Immediate Experimental Group
Participants will immediately begin an experimental supervised moderate-intensity treadmill walking intervention program.
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Participants will attend 3 treadmill walking sessions/week for 8 weeks (24 total)-exercising at 60-75% of their heart rate (HR) reserve monitored using Polar FT1 HR monitors.
All walking sessions will take place using treadmills within the West Bank Office Building on the UMN-Twin Cities campus.
Each walking session will be 30 min long during intervention weeks 1-4 and 45 min each during weeks 5-8.
This exercise dose is equivalent to that employed in the only two human studies of exercise and the gut microbiome in addition to the dosage observed necessary to yield meaningful cardiometabolic changes.
After the 4th- and 8th-weeks of the intervention, participants in both study groups will again undergo outcome assessments
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NO_INTERVENTION: Delayed Intervention Control Group
Participants will be asked to maintain their usual physical activity during the initial 8-week intervention period but will undergo all data collection procedures.
Following the initial intervention period, these participants will be given the option to complete the 8-week intervention, with identical data collection procedures employed.
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What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Change in Shannon Index (Alpha-Diversity) as Reflected in Change in Operational Taxonomic Units
Time Frame: Baseline, 8 weeks
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Alpha diversity is the mean species diversity of a local site, which, in this study, is the fecal sample provided by participants used as a proxy for sampling the gut microbiome.
The Shannon Index is based on the weighted geometric mean of the proportional abundances of the types of microbes.
Change in alpha diversity will be reported as change in operational taxonomic units (OTUs).
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Baseline, 8 weeks
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Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Change in Plasma LDL Concentrations
Time Frame: Baseline, 8 weeks
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Participants will have blood drawn after fasting over night or for at least 8 hours.
Plasma low density lipoprotein (LDL) concentrations will be determined using standard laboratory assay methods.
Concentrations will be reported in mg/dl.
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Baseline, 8 weeks
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Change in Plasma HDL Concentrations
Time Frame: Baseline, 8 weeks
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Participants will have blood drawn after fasting over night or for at least 8 hours.
Plasma high density lipoprotein (HDL) concentrations will be determined using standard laboratory assay methods.
Concentrations will be reported in mg/dl.
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Baseline, 8 weeks
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Change in Plasma Total Cholesterol Concentrations
Time Frame: Baseline, 8 weeks
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Participants will have blood drawn after fasting over night or for at least 8 hours.
Plasma total cholesterol concentrations will be determined using standard laboratory assay methods.
Concentrations will be reported in mg/dl.
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Baseline, 8 weeks
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Change in Plasma Insulin Concentrations
Time Frame: Baseline, 8 weeks
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Participants will have blood drawn after fasting over night or for at least 8 hours.
Plasma insulin concentrations will be determined using standard laboratory assay methods.
Concentrations will be reported in mmol/l.
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Baseline, 8 weeks
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Change in Plasma Glucose Concentrations
Time Frame: Baseline, 8 weeks
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Participants will have blood drawn after fasting over night or for at least 8 hours.
Plasma glucose concentrations will be determined using standard laboratory assay methods.
Concentrations will be reported in mg/dl.
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Baseline, 8 weeks
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Change in Plasma CRP Concentrations
Time Frame: Baseline, 8 weeks
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Participants will have blood drawn after fasting over night or for at least 8 hours.
Plasma C-reactive protein (CRP) concentrations will be determined using standard laboratory assay methods.
Concentrations will be reported in mg/l.
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Baseline, 8 weeks
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Change in Resting Blood Pressure - Systolic
Time Frame: Baseline, 8 weeks
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Resting blood pressure will be measured using an Omron Automatic BP Cuff and reported in mmHG.
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Baseline, 8 weeks
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Change in Resting Blood Pressure - Diastolic
Time Frame: Baseline, 8 weeks
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Resting blood pressure will be measured using an Omron Automatic BP Cuff and reported in mmHG.
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Baseline, 8 weeks
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Change in Waist Circumference
Time Frame: Baseline, 8 weeks
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Waist circumference will be measured using anthropometric tape and reported in cm.
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Baseline, 8 weeks
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Change in Body Weight
Time Frame: Baseline, 8 weeks
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Body weight will be measured using a Tanita Body Composition and Weight scale and reported in kg.
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Baseline, 8 weeks
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Change in Body Fat Percentage
Time Frame: Baseline, 8 weeks
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Body fat percentage will be measured using a Tanita Body Composition and Weight scale and reported as a percentage.
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Baseline, 8 weeks
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Collaborators and Investigators
Sponsor
Study record dates
Study Major Dates
Study Start (ACTUAL)
Primary Completion (ACTUAL)
Study Completion (ACTUAL)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (ACTUAL)
Study Record Updates
Last Update Posted (ACTUAL)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Additional Relevant MeSH Terms
Other Study ID Numbers
- STUDY00007532
Plan for Individual participant data (IPD)
Plan to Share Individual Participant Data (IPD)?
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
Studies a U.S. FDA-regulated device product
product manufactured in and exported from the U.S.
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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