- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT04280315
Investigation of the Skin Barrier in Patients With Type 1 Diabetes
Investigation of the Skin Barrier in Patients With Type 1 Diabetes - a Case-control Study
Study Overview
Status
Conditions
Detailed Description
The skin is a crucial barrier against external allergens and irritants. Therefore the function of the skin barrier is central regarding evolvement of eczema. In patients with type 1 diabetes (T1D) using insulin pumps and/or glucose sensor, eczema is unfortunately a frequent reaction. The skin consists of three layers: epidermis, dermis and subcutaneous fat, whereas epidermis is most determining regarding skin barrier. Epidermis can further be subdivided, where stratum corneum (SC) is the outer part, which means most regarding vulnerability towards external allergens.
The function of the skin barrier can be investigated by non-invasive methods using transepidermal water loss (TEWL), which shows the water vapour flux from the skin. Besides SC can be determined using the tape strip method, where few SC cells are peeled off and thereby can be analyzed with mass-spectrometry. Natural moisturizing factors (NMF) is though one of the very important functionalities of the SC, which are determined by the amount of 2-pyrrolidone-5-carboxylic acid (PCA). Besides, the lipid distribution, presence of antimicrobial peptide and cytokine response give as well important information regarding the skin barrier.
Dermatological manifestations of T1D are quite frequent and have been shown to be related to glycemic control, placing dermatological manifestations as a precursor of other microvascular complications11. There is a lack of studies regarding skin barrier function in patients with T1D, and especially in a pediatric study population. Therefore, this case-control study will be conducted investigating skin barrier function in pediatric patients with T1D compared to healthy controls in order to both meet this lack of knowledge, but as well to improve the prevention of skin problems to devices used in T1D-treatment.
The examination program of just one visit per participant consists of:
- TEWL-measure at forearm and buttock
- Buccal swab regarding filaggrin gene status
- Microbiological swab from the skin at forearm and buttock
- 2X11 tape strips from the skin at foream and buttock to be analyzed for NMF, Lipid, Cytokines, Antimicrobial peptide and Dermal Topographical Index as well as protein content using SquameScan
- Measure of skin PH, hydration and sebum content at forearm and buttock
- Capillary Blood Glucose
- Survey regarding atopic and dermatological symptoms
Study Type
Enrollment (Actual)
Contacts and Locations
Study Locations
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Region Hovedstaden
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Gentofte, Region Hovedstaden, Denmark, 2820
- Steno Diabetes Center Copenhagen
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Herlev, Region Hovedstaden, Denmark, 2730
- Department of Pediatrics, Herlev Hospital
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Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Genders Eligible for Study
Sampling Method
Study Population
Description
Cases:
Inclusion Criteria:
- Diagnosed with type 1 diabetes, more than 6 months ago
Exclusion Criteria:
- Skin lesions at both buttocks and forearms so investigation can not be done
- Known skin diseases with decreased barrier function like atopic dermatitis, psoriasis and allergic contact dermatitis
- Active infectious disease
- Use of immunosuppressive medication e.g. steroids
- Lack of language competence in Danish
Controls:
Inclusion Criteria:
- Diagnosed with Tourettes or Headache OR sibling for patients in the 1813-clinic at Pediatric Emergency Toom OR Hospitalized patients at Pediatric department OR Parents to alle these
Exclusion Criteria are equal to exclusion criteria in cases
Study Plan
How is the study designed?
Design Details
Cohorts and Interventions
Group / Cohort |
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Cases
Cases are patients with type 1 diabetes in three age strata:
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Controls
Controls are age and sexmatched with the cases and are recruited from the neuropediatric clinic at Herlev Hospital as well as relatives and parents of patients in the whole Pediatric Department
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What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
TEWL
Time Frame: Baseline
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Transepidermal Water Loss measured by an Aquaflux AF1000
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Baseline
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Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Skin barrier properties described with Tape Strips
Time Frame: Baseline
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Tape strips will be analyzed according to Natural Moisturizing Factors
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Baseline
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Filaggrin mutation Status
Time Frame: Baseline
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Filaggrin DNA status regarding common SNPs in DNA
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Baseline
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Skin pH
Time Frame: Baseline
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Measured with DermaUnit SSC3 Skin pH-Meter
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Baseline
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Skin hydration
Time Frame: Baseline
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Measured with DermaUnit SSC3 Corneometer
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Baseline
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Sebum content in skin
Time Frame: Baseline
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Measured with DermaUnit SSC3 Sebumeter
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Baseline
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Collaborators and Investigators
Sponsor
Publications and helpful links
Helpful Links
Study record dates
Study Major Dates
Study Start (Actual)
Primary Completion (Actual)
Study Completion (Actual)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Actual)
Study Record Updates
Last Update Posted (Actual)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Keywords
Additional Relevant MeSH Terms
Other Study ID Numbers
- SkinBarrier_30092019
Plan for Individual participant data (IPD)
Plan to Share Individual Participant Data (IPD)?
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
Studies a U.S. FDA-regulated device product
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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