GnRH Antagonist Pre-treatment in the Early Follicular Phase for Resolution of a Baseline Functional Ovarian Cyst

GnRH Antagonist Pre-treatment in the Early Follicular Phase for Resolution of a Baseline Functional Ovarian Cyst - A Proof-of-concept Pilot Study

In this study we would like to examine the effect of GnRH antagonist administration at the beginning of the follicular phase in patient presenting with a simple ovarian cyst 25-50 mm. The aim of this intervention is to allow a spontaneous regression of the ovarian cyst (if in nature) while ensuring a pituitary downregulation to prevent the beginning of a leading follicle recruitment. As previous studies using GnRH antagonist pre-treatment prior to GT initiation for other purposes demonstrated positive results (including different patient population) , no deleterious effects are expected.

Study Overview

• Status: Recruiting
• Conditions: Ovarian Cysts; IVF
• Intervention / Treatment: Drug: Cetrorelix Acetate, Ganerelix Acetate

Detailed Description

Reports on the effect of a baseline ovarian cyst on IVF cycle cancellation and outcome are scare and inconsistent. A cystic structure of the ovary may be functional or non-functional. Functional ovarian cysts are usually created by disruption of normal ovulation with the accumulation of intrafollicular fluid. Hormonal dysfunction prior to ovulation results in expansion of the follicular antrum with serous fluid. These cysts may be more frequent using Progesterone only contraceptives, Levonogetstrel-containing intrauterine device and following controlled ovarian stimulation during fertility treatments . Most functional cysts regress spontaneously within the first few days of menstruation or within the first 1-2 cycles and up to 6 months.

An ovarian cystic structure, even if hormonally inactive, may interfere with ovarian function during ovulation induction. These cysts may have a mechanical effect by reducing the space for growing follicles or by impairing ovarian blood supply. This may result in a lower number of oocytes and poor embryo quality, or utilization of higher doses of gonadotropins(GT) to reach the same oocyte yield.

Several studies have investigated the outcome of GnRH antagonist supplementation at the beginning of the menstrual cycle prior to GT stimulation mainly for the purpose of synchronization of antral follicles preventing a premature follicular recruitment. The delayed start protocol which combines estradiol priming followed by GnRH antagonist for 7 days at the beginning of menses prior to GT administration to further synchronize antral follicles improved ovarian response in poor responders and reduced cycle cancellation rate with no significant effect on pregnancy rates. A preliminary study investigated the effect of three day administration of GnRH antagonist at the beginning of the follicular phase prior to GT stimulation in normal responders regardless of baseline hormonal levels and found a trend towards increase in clinical pregnancy rate, and similar profiles of early embryo development, compared to standard fixed GnRH antagonist protocol.

Patients receiving an antagonist protocol usually start their stimulation on day 2-3 of menses. A baseline US and hormonal blood test are performed to determine adequacy of cycle start.

Patients presenting with a simple ovarian cyst larger then 25-30 mm in our unit, even in the presence of normal baseline estradiol levels are deferred to start their treatment in the following menstrual cycle as these structures may negatively affect treatment outcome. It is possible that these cystic structures, most being remnants of previous cycle, would have resolved spontaneously within a few days, but since GT treatment is to be started on the 2-3 day of the cycle to prevent leading follicle recruitment, we cannot wait to find out whether cyst has resolved and are inclined to postpone treatment to the following menstrual cycle.

Study aims: In this study we would like to examine the effect of GnRH antagonist administration at the beginning of the follicular phase in patient presenting with a simple ovarian cyst 25-50 mm. The aim of this intervention is to allow a spontaneous regression of the ovarian cyst (if in nature) while ensuring a pituitary downregulation to prevent the beginning of a leading follicle recruitment. As previous studies using GnRH antagonist pre-treatment prior to GT initiation for other purposes demonstrated positive results (including different patient population) , no deleterious effects are expected.

Materials and Methods: This is a proof-of-concept pilot study. Patients will be recruited at the reproductive medicine unit of Shamir Medical Center, Israel.

Study population: Patients undergoing controlled ovarian hyper stimulation at the IVF unit, Shamir medical center. Planned recruitment of 15 patients.

Sample size: This is a proof-of-concept pilot study to evaluate the feasibility and potential effectiveness of the intervention. A sample size of 15 patients will allow detecting the potential for use of cycles that would have been cancelled using standard protocols. As the comparator is mandatory cancellation, i.e. 0% use of cycles, this sample size is sufficient.

Study protocol:

Patients treated at the IVF unit at Shamir Medical Center planned for an antagonist cycle will undergo blood test and US exam at day 2-3 of their menstrual cycle. Patients will be recruited if a simple ovarian cyst > 25mm and < 50 mm is demonstrated upon US exam in the presence of E2 < 220 pmol/L. Following the initial US exam in the morning, if a simple cyst 25-50 mm will be demonstrated, the patient will receive a form explaining that her protocol may be changed according to blood test results with three possible options: 1. Continuation with the original protocol 2. Cancellation 3. Change in protocol as part of a research if she agrees to participate. As patients undergo blood draws in the morning, with results available in the afternoon, the initial recruitment will be performed over the phone with a verbal informed consent documented in the patients' medical file. A signed informed consent received at the following clinic visit.

Patients will receive a verbal explanation over the phone and following consent will start GnRH antagonist injections [Cetrotide (cetrolix) or Orgalutran (ganirelix) - depending on their primary prescription] for 3 days by which time a second blood test (for estradiol, progesterone, LH and FSH) and US exam will be performed.

• If cyst size has decreased to < 25 mm, and estradiol levels have remained < 220 pmol/L then GT stimulation will be started according to the original protocol.
• If cyst size has decreased in more than 10 mm mm but is still above 25mm, and estradiol levels have remained < 220 pmol/L additional 3 days treatment with Cetrotide (cetrolix) or Orgalutran (ganirelix) will be given.
• In case of no change/increase, the cycle will be cancelled. The patient will receive oral contraceptives for 2-3 weeks to time the next cycle and will start a new cycle once the current cyst resolves. The time to cyst resolution will be recorded.

When treatment is continued, the remnant of the cystic structure will be separately followed. Treatment outcomes will be recorded, including the number of follicles on both ovaries, number of mature oocytes of expected mature oocytes and number of embryos. Cystic outcome (if aspirated as part of the ovum pickup) will be recorded separately. In patients with a previous or a subsequent cycle with similar GT dosages, cycle outcomes will be compared to the cycle with the intervention.

Risks to subjects: No anticipated risks over regular stimulation protocol side effects.

Statistical analyses: Descriptive statistics will be used.

• Study Type: Interventional
• Enrollment (Anticipated): 15
• Phase: Phase 4

Contacts and Locations

• Study Contact: Name: Michal Youngster, MD; Phone Number: +972506430111; Email: michalyo@gmail.com
• Study Contact Backup: Name: Ariel Hourvitz, MD; Phone Number: +972526666063; Email: ariel@hourvitz.co.il

Study Locations

• Israel
  • Be'er Ya'aqov, Israel
    • Recruiting
    • Shamir Medical Center
    • Contact: Michal youngster, MD; Phone Number: 972-506430111; Email: michalyo@gmail.com
    • Contact: Ariel Hourvitz, MD; Phone Number: 972-526666063; Email: ariel@Hourvitz.co.il

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 45 years (Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: Female

Description

Inclusion Criteria:

1. Antagonist protocol.
2. Presentation for cycle initiation on day 2-3 of the menstrual cycle with a simple ovarian cyst >25 mm and < 50mm, and estradiol levels < 220 pmol/L.

Exclusion Criteria:

1. Inability to provide informed consent.
2. Oocyte donor

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: N/A
• Interventional Model: Single Group Assignment
• Masking: None (Open Label)

Number of Arms

1

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Participants; Patients will start GnRH antagonist injections [Cetrotide (cetrolix) or Orgalutran (ganirelix) - depending on their primary prescription] for 3-6 days	Drug: Cetrorelix Acetate, Ganerelix Acetate; 3-6 days of 0.25 mg starting on day 2-3 of the menstrual cycle

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Cyst resolution	Number of participants with a resolution/decrease in size of the cystic structure following GnRH antagonist.	2 years
Number of oocytes retrieved	cycle outcome - number of oocytes retrieved, of participants following the administration of the GnRH antagonist	2 years

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Number of patients which had an oocyte in the aspirated cystic content.	cystic content	2 years
Comparison of cycle outcome - oocyte number compared to a previous/future cycle cycle outcome compared to a previous cycle	Comparison to a similar cycle of the same patients using similar GT dosages - number of oocytes retrieved.	2 years

Collaborators and Investigators

• Sponsor: Assaf-Harofeh Medical Center

Study record dates

Study Major Dates

• Study Start (Actual): May 1, 2021
• Primary Completion (Anticipated): March 1, 2022
• Study Completion (Anticipated): March 1, 2023

Study Registration Dates

• First Submitted: February 20, 2021
• First Submitted That Met QC Criteria: July 29, 2021
• First Posted (Actual): August 6, 2021

Study Record Updates

• Last Update Posted (Actual): October 13, 2021
• Last Update Submitted That Met QC Criteria: October 5, 2021
• Last Verified: October 1, 2021

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Neoplasms; Endocrine System Diseases; Ovarian Diseases; Adnexal Diseases; Gonadal Disorders; Pathological Conditions, Anatomical; Ovarian Cysts; Cysts; Physiological Effects of Drugs; Hormones, Hormone Substitutes, and Hormone Antagonists; Hormone Antagonists; Reproductive Control Agents; Fertility Agents, Female; Fertility Agents; Cetrorelix
• Other Study ID Numbers: 0024-21-ASF

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: Yes
• Studies a U.S. FDA-regulated device product: No
• product manufactured in and exported from the U.S.: Yes