MicroRNA-1 (miRNA-1) and MicroRNA-133a (miRNA-133a) Levels After Acute Exercise in Ultimate Frisbee Athletes

October 11, 2023 updated by: Mehmet Alperen Pekdaş, Yeditepe University

Investigation of MicroRNA-1 (miRNA-1) and MicroRNA-133a (miRNA-133a) Levels After Acute Neuromuscular Exercise in Ultimate Frisbee Athletes

"The goal of this clinical trial is to investigate the levels of microRNA-1 (miRNA-1) and microRNA-133a (miRNA-133a) after acute neuromuscular exercise (NME) in human saliva.

The main questions it aims to answer are:

  • How do miRNA-1 and miRNA-133a levels change in response to acute neuromuscular exercise in human saliva?
  • Will the chosen training method have an acute effect in ultimate frisbee athletes?

Participants will:

  • Undergo acute neuromuscular exercise (NME).
  • Provide saliva samples for miRNA analysis.

Researchers will compare:

MiRNA-1 and miRNA-133a levels before and after acute neuromuscular exercise (NME) to determine if exercise induces any significant changes in these microRNA levels."

Study Overview

Status

Recruiting

Conditions

Intervention / Treatment

Detailed Description

The genetic material in humans and other living things is DNA, and the information in DNA is transcribed into RNA, and RNA translates this information into protein through translation. More than 98% of RNA cannot be translated into protein this untranslated part is called non-coding RNA. Of the non-coding RNAs, micro-RNAs (miRNAs) are short RNAs of approximately 21-23 nucleotides in length that do not code for proteins and that control gene expression after transcription. miRNAs can partially bind to target messenger RNAs (mRNAs), suppressing protein production or silencing gene expression by splicing target mRNAs. miRNAs enable cell differentiation and development by using the translational repression mechanism. In addition to these roles, miRNAs play a role in many points such as cellular metabolism, repair mechanisms, signaling, cell division, and apoptosis pathways. Studies also identify miRNAs as essential intracellular mediators of processes involved in exercise adaptation, including angiogenesis, inflammation, mitochondrial metabolism, cardiac and skeletal muscle contraction force generation, and tissue hypertrophy. There is evidence that the miRNA may play an important role in regulating the plasticity of the skeletal muscles, such as the hypertrophy of the skeletal muscles, or in regulating the functional properties of the muscle. More than 2000 miRNAs have been identified in humans, and some of them are found and function in the human body in a tissue-specific manner. For example, miRNAs, mostly found in muscle tissue and therefore called myomiRs, are involved in proliferation, muscle cell differentiation, metabolism, and hypertrophy in skeletal and cardiac muscles. The myomiR class includes miR-1, miR-133a, miR-133b, miR-206, miR-208a, miR-208b, miR-486, and miR-499. In the literature, studies examine miRNA levels before and after using various exercise modalities. It has been stated that different miRNAs provide responses such as ventricular compliance, hypertrophy, angiogenesis, and mitochondrial biogenesis after acute or chronic exercise. It has been shown in many studies that these responses, decreased or increased miRNA levels after exercise, are related to the target genes they affect, such as IGF-1, PGC-1α, MAPK, TGF-β, COL1A1, and FOXJ3.

The aim of the study is to investigate the levels of miRNA-1 and miRNA-133a levels after acute neuromuscular exercise (NME) in human saliva.

Study Type

Observational

Enrollment (Estimated)

34

Contacts and Locations

This section provides the contact details for those conducting the study, and information on where this study is being conducted.

Study Contact

Study Contact Backup

Study Locations

  • Turkey
      • Istanbul, Turkey, 34755
        • Recruiting
        • Yeditepe University
        • Contact:
        • Contact:
        • Principal Investigator:
          • Mehmet A Pekdaş, Research Assistant
        • Sub-Investigator:
          • Feryal Subaşı, Prof. Dr.
        • Sub-Investigator:
          • Seda Güleç Yılmaz, Assoc. Prof. Dr.
        • Sub-Investigator:
          • Gökhan Meriç, Prof. Dr.
        • Sub-Investigator:
          • Turgay İsbir, Prof. Dr.

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

  • Adult

Accepts Healthy Volunteers

Yes

Sampling Method

Non-Probability Sample

Study Population

Ultimate Frisbee team athletes of a university

Description

Inclusion Criteria:

  • Not suffering from an acute illness
  • Not having had an orthopedic injury in the last 12 weeks may prevent them from exercising
  • Volunteering to participate in the study

Exclusion Criteria:

  • Presence of an acute disease
  • Having an orthopedic injury that may prevent exercise in the last 12 weeks
  • Regular use of medication and/or dietary supplements
  • Presence of a chronic & systemic disease
  • Not volunteering to participate in the study

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Time Frame
Determination of miRNA-1 and miRNA-133a levels before and after acute neuromuscular exercise
Time Frame: Approximately 1.5 hours for each participant
Approximately 1.5 hours for each participant

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

Yeditepe University

Investigators

  • Principal Investigator: Turgay İsbir, Prof. Dr., Yeditepe University

Publications and helpful links

The person responsible for entering information about the study voluntarily provides these publications. These may be about anything related to the study.

General Publications

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start (Actual)

October 11, 2023

Primary Completion (Estimated)

April 1, 2024

Study Completion (Estimated)

May 31, 2024

Study Registration Dates

First Submitted

September 18, 2023

First Submitted That Met QC Criteria

October 11, 2023

First Posted (Actual)

October 16, 2023

Study Record Updates

Last Update Posted (Actual)

October 16, 2023

Last Update Submitted That Met QC Criteria

October 11, 2023

Last Verified

October 1, 2023

More Information

Terms related to this study

Keywords

Other Study ID Numbers

  • 14

Plan for Individual participant data (IPD)

Plan to Share Individual Participant Data (IPD)?

UNDECIDED

Drug and device information, study documents

Studies a U.S. FDA-regulated drug product

No

Studies a U.S. FDA-regulated device product

No

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

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