Enhancing CAR-T Cell Therapy Efficacy in B-cell Lymphoma Via Chidamide and PD-1 Inhibitor Combination.

Chidamide-based Combination With PD-1 Blockade: A Synergistic Strategy to Improve CAR-T Cell Therapy Outcomes for B-cell Lymphoma.

B-cell non-Hodgkin lymphoma (B-NHL) is one of the most common malignancies in China, with approximately 100,000 new cases diagnosed annually. Although immunochemotherapy, novel small-molecule targeted agents, and hematopoietic stem cell transplantation have significantly improved outcomes for patients with B-cell malignancies, nearly half of patients still experience drug resistance and relapse. In high-risk aggressive B-cell lymphoma, the 5-year survival rate remains around 50%. Previous clinical guidelines recommended autologous hematopoietic stem cell transplantation as first-line consolidation therapy for high-risk patients; however, multiple studies have demonstrated that even after autologous transplantation, nearly half of these patients relapse and succumb to the disease.

Chimeric antigen receptor T (CAR-T) cell therapy has achieved objective response rates of approximately 50% in relapsed/refractory lymphoma, particularly in B-cell subtypes. Nevertheless, limitations such as tumor immune antigen escape, immunosuppressive effects of the tumor microenvironment (TME) on CAR-T cells, and T-cell exhaustion continue to restrict the durability and efficacy of CAR-T-mediated cytotoxicity.

This study evaluates the incorporation of chidamide (an HDAC inhibitor) combined with a PD-1 inhibitor as maintenance therapy following CAR-T cell immunotherapy in patients with relapsed/refractory high-risk aggressive B-cell lymphoma. By implementing an "early intervention" strategy-prompt administration of CAR-T cell therapy after induction treatment for relapsed/refractory high-risk aggressive B-cell lymphoma-and subsequent maintenance with chidamide plus a PD-1 inhibitor, the approach aims to reduce relapse rates and improve overall survival. These strategies are intended to address the current unmet clinical need for improved outcomes in relapsed/refractory high-risk aggressive B-cell lymphoma, where prognosis remains poor despite existing therapies.

Study Overview

• Status: Recruiting
• Conditions: Relapsed or Refractory Diffuse Large B-Cell Lymphoma (R/R DLBCL)
• Intervention / Treatment: Drug: CAR-T Cell Therapy + Chidamide and PD-1 Inhibitor Maintenance

• Study Type: Interventional
• Enrollment (Estimated): 30
• Phase: Phase 2

Contacts and Locations

• Study Contact: Name: Li-Ping Dou, Dr.; Phone Number: 86-010-66937232; Email: lipingruirui@163.com

Study Locations

• China
  • Beijing Municipality
    • Beijing, Beijing Municipality, China, 100853
      • Recruiting
      • Chinese PLA General Hospital

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult; Older Adult
• Accepts Healthy Volunteers: No

Description

Inclusion Criteria

-

The patient must meet all of the following inclusion criteria:

1. Histologically or cytologically confirmed CD19 and/or CD22-positive large B-cell lymphoma (LBCL) according to the WHO 2016 classification, including diffuse large B-cell lymphoma (DLBCL), high-grade B-cell lymphoma (HGBL), and related entities, with one of the following:

   1. Partial response (PR) after induction therapy with a standard first-line chemotherapy regimen (e.g., R-CHOP for 4-6 cycles); or
   2. Complete response (CR) after standard first-line induction therapy, but with high-risk features present at initial diagnosis.

2. Presence of high-risk features at initial diagnosis, defined as at least one of the following:

   1. High-grade B-cell lymphoma with MYC and BCL2 and/or BCL6 rearrangements ("double-hit" or "triple-hit") confirmed by fluorescence in situ hybridization (FISH);
   2. High-grade B-cell lymphoma with 11q aberration (Burkitt-like lymphoma with 11q aberration);
   3. International Prognostic Index (IPI) score of 2-5; age-adjusted IPI (aa-IPI) score of 2-3; or National Comprehensive Cancer Network-IPI (NCCN-IPI) score of 4-8;
   4. CD5 positivity by immunohistochemistry;
   5. Dual expression of MYC and BCL2 by immunohistochemistry (recommended thresholds: MYC ≥ 40% and BCL2 ≥ 50%);
   6. TP53 mutation detected by gene sequencing;
   7. Molecular subtype MCD or N1 by next-generation sequencing (NGS);

   8. Relapsed/refractory B-cell lymphoma, meeting one of criteria ①-④ plus criterion ⑤:

      • Less than 50% tumor reduction or disease progression after ≥4 cycles of standardized chemotherapy;

        • Relapse within 6 months after achieving CR with standard regimen;

          • ≥2 relapses after CR;

            • Relapse after hematopoietic stem cell transplantation;

              • Must have received adequate prior therapy, including at least an anti-CD20 monoclonal antibody and anthracycline-containing combination chemotherapy.
3. Age 18 to 85 years, male or female.
4. Eastern Cooperative Oncology Group (ECOG) performance status of 0-2.
5. Expected survival of >3 months from the date of signing informed consent.
6. Hemoglobin (HGB) ≥60 g/L (transfusion permitted).
7. Absolute neutrophil count (ANC) ≥1,000/μL and platelet count ≥45,000/μL.

8. Adequate hepatic, renal, cardiac, and pulmonary function, meeting **all** of the following:

   1. Total bilirubin (TBIL) ≤1.5 × upper limit of normal (ULN) (except for patients with Gilbert's syndrome);
   2. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) ≤2.5 × ULN;
   3. Serum creatinine (Cr) ≤1.5 × ULN **or** creatinine clearance (CCr) ≥60 mL/min (estimated by Cockcroft-Gault formula);
   4. Left ventricular ejection fraction (LVEF) ≥50% by echocardiogram (ECHO), with no pericardial effusion and no clinically significant arrhythmias;
   5. Baseline oxygen saturation by pulse oximetry >92% on room air;
   6. No clinically significant pleural effusion.

Exclusion Criteria:

• Patients eligible for CAR-T cell immunotherapy must **NOT** meet any of the following exclusion criteria:

  1. Prior treatment with any form of chimeric antigen receptor (CAR) T-cell therapy or other genetically modified T-cell therapy.
  2. History of severe immediate-type hypersensitivity reaction to aminoglycoside antibiotics or other drugs.

  3. Known history of human immunodeficiency virus (HIV) infection, active hepatitis B virus (HBV) infection, or any uncontrolled active systemic infection requiring intravenous antibiotics.

     (Active HBV infection is defined as meeting **all** of the following: a) HBV DNA ≥ 2000 IU/mL; b) ALT ≥ 2 × upper limit of normal (ULN); c) hepatitis not attributable to other causes such as the underlying disease or medications. Patients with active HBV at initial diagnosis who achieve non-active HBV status after adequate anti-HBV treatment may be eligible under continued effective anti-HBV therapy.)

  4. Non-hematologic malignancy-related hepatic or renal impairment, including any of the following: ALT > 3 × ULN, AST > 3 × ULN, total bilirubin (TBIL) > 2 × ULN, or creatinine clearance < 30 mL/min.
  5. History of myocardial infarction, percutaneous coronary intervention (including coronary angioplasty or stenting), unstable angina, active arrhythmia, or other clinically significant cardiovascular disease within the past 12 months.
  6. Any other serious medical condition that, in the opinion of the investigator, may interfere with the study treatment or increase risk to the patient (e.g., poorly controlled diabetes, active peptic ulcer disease, severe respiratory or circulatory disease, severe autoimmune disease, congenital immunodeficiency, uncontrolled severe infection, or other conditions with high risk of clinical deterioration).
  7. History of severe immediate-type hypersensitivity reaction to any medication required during the treatment process, or history of severe allergy to biologics (including antibiotics).
  8. Female patients who are pregnant or breastfeeding (preconditioning chemotherapy regimen poses potential risk to the fetus or infant).
  9. In the opinion of the investigator, the patient is unlikely to comply with all required study visits, procedures, or long-term follow-up; has poor willingness or ability to participate and cooperate fully; or has insufficient compliance (as judged by the patient and/or family).
  10. History of other malignancy, unless the patient has been disease-free and has received no antitumor therapy for at least 3 years (exceptions: non-melanoma skin cancer, and carcinoma in situ of the cervix, bladder, or breast).
  11. Receipt of a live vaccine within 6 weeks prior to initiation of the preconditioning regimen.
  12. Major surgery (excluding lymph node biopsy) within the past 14 days, or anticipated need for major surgery during the treatment period.
  13. Any other serious physical or psychiatric illness, or clinically significant laboratory abnormality, that may increase the risk associated with study participation, interfere with the interpretation of study results, or render the patient unsuitable for participation in the opinion of the investigator.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: N/A
• Interventional Model: Single Group Assignment
• Masking: None (Open Label)

Number of Arms

1

Arms and Interventions

Participant Group / Arm

Intervention / Treatment

Experimental: CAR-T + Chidamide/PD-1 Maintenance in R/R High-Risk DLBCL

Drug: CAR-T Cell Therapy + Chidamide and PD-1 Inhibitor Maintenance; Patients will receive maintenance therapy consisting of Chidamide combined with a PD-1 inhibitor following CAR-T cell infusion. This intervention is designed to upregulate target antigen expression on tumor cells and mitigate antigen escape. By synergistically enhancing the cytotoxic activity and persistence of CAR-T cells, the regimen aims to reduce the risk of relapse and improve long-term clinical outcomes

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
1-year progression free survival rate (1-year-PFSR)	1 years after treatment

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
overall survival (OS)		2 years after treatment
progression free survival (PFS)		2 years after treatment
time to progression (TTP)		2 years after treatment
disease free survival (DFS)		2 years after treatment
duration of response (DOR)		2 years after treatment
event free survival (EFS)		2 years after treatment
recurrence rate		2 years after treatment
safety	Evaluate post-CAR-T cell infusion adverse events by analyzing the number of cases, incidence rates, and severity grades of the following key immunotherapy-related toxicities as recorded: Cytokine release syndrome (CRS) Immune effector cell-associated neurotoxicity syndrome (ICANS) Hematologic toxicity Organ toxicity and other immune-related adverse events associated with CAR-T cell therapy.	2 years after treatment
CAR-T cell kinetic parameters in peripheral blood	CAR gene copy number Peak CAR-T cell concentration (Cmax) Time to peak concentration (Tmax) Area under the concentration-time curve from day 0 to day 28 (AUC₀-₂₈d / AUC₂₈d)	2 years after treatment

Other Outcome Measures

Outcome Measure	Time Frame
Exploratory analysis of the correlation between minimal residual disease (MRD) status, as determined by circulating tumor DNA (ctDNA)-based MRD detection, and clinical prognosis in high-risk patients.	2 years after treatment

Collaborators and Investigators

• Sponsor: Daihong Liu

Study record dates

Study Major Dates

• Study Start (Actual): May 15, 2025
• Primary Completion (Estimated): May 15, 2027
• Study Completion (Estimated): May 15, 2027

Study Registration Dates

• First Submitted: March 18, 2026
• First Submitted That Met QC Criteria: March 18, 2026
• First Posted (Actual): March 24, 2026

Study Record Updates

• Last Update Posted (Actual): March 24, 2026
• Last Update Submitted That Met QC Criteria: March 18, 2026
• Last Verified: May 1, 2025

More Information

Terms related to this study

• Keywords: PD-1 inhibitor; Chidamide; CAR-T therapy; Relapsed or Refractory Diffuse Large B-Cell Lymphoma (R/R DLBCL)
• Additional Relevant MeSH Terms: Pathologic Processes; Neoplasms; Disease Attributes; Immune System Diseases; Neoplasms by Histologic Type; Lymphatic Diseases; Lymphoproliferative Disorders; Immunoproliferative Disorders; Lymphoma, Non-Hodgkin; Lymphoma, B-Cell; Lymphoma; Pathological Conditions, Signs and Symptoms; Hemic and Lymphatic Diseases; Recurrence; Lymphoma, Large B-Cell, Diffuse; Investigative Techniques; Therapeutics; Biological Therapy; Immunologic Techniques; Immunomodulation; Adoptive Transfer; Immunization, Passive; Immunization; Immunotherapy; N-(2-amino-5-fluorobenzyl)-4-(N-(pyridine-3-acrylyl)aminomethyl)benzamide; Immunotherapy, Adoptive
• Other Study ID Numbers: M2025-222-01

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No