Functional analysis via standardized whole-blood stimulation systems defines the boundaries of a healthy immune response to complex stimuli
Darragh Duffy, Vincent Rouilly, Valentina Libri, Milena Hasan, Benoit Beitz, Mikael David, Alejandra Urrutia, Aurélie Bisiaux, Samuel T Labrie, Annick Dubois, Ivo G Boneca, Cécile Delval, Stéphanie Thomas, Lars Rogge, Manfred Schmolz, Lluis Quintana-Murci, Matthew L Albert, Milieu Intérieur Consortium, Laurent Abel, Andres Alcover, Philippe Bousso, Ana Cumano, Marc Daëron, Cécile Delval, Caroline Demangel, Ludovic Deriano, James Di Santo, Françoise Dromer, Gérard Eberl, Jost Enninga, Antonio Freitas, Ivo Gomperts-Boneca, Serge Hercberg, Olivier Lantz, Claude Leclerc, Hugo Mouquet, Sandra Pellegrini, Stanislas Pol, Lars Rogge, Anavaj Sakuntabhai, Olivier Schwartz, Benno Schwikowski, Spencer Shorte, Vassili Soumelis, Frédéric Tangy, Eric Tartour, Antoine Toubert, Marie-Noëlle Ungeheuer, Lluis Quintana-Murci, Matthew L Albert, Darragh Duffy, Vincent Rouilly, Valentina Libri, Milena Hasan, Benoit Beitz, Mikael David, Alejandra Urrutia, Aurélie Bisiaux, Samuel T Labrie, Annick Dubois, Ivo G Boneca, Cécile Delval, Stéphanie Thomas, Lars Rogge, Manfred Schmolz, Lluis Quintana-Murci, Matthew L Albert, Milieu Intérieur Consortium, Laurent Abel, Andres Alcover, Philippe Bousso, Ana Cumano, Marc Daëron, Cécile Delval, Caroline Demangel, Ludovic Deriano, James Di Santo, Françoise Dromer, Gérard Eberl, Jost Enninga, Antonio Freitas, Ivo Gomperts-Boneca, Serge Hercberg, Olivier Lantz, Claude Leclerc, Hugo Mouquet, Sandra Pellegrini, Stanislas Pol, Lars Rogge, Anavaj Sakuntabhai, Olivier Schwartz, Benno Schwikowski, Spencer Shorte, Vassili Soumelis, Frédéric Tangy, Eric Tartour, Antoine Toubert, Marie-Noëlle Ungeheuer, Lluis Quintana-Murci, Matthew L Albert
Abstract
Standardization of immunophenotyping procedures has become a high priority. We have developed a suite of whole-blood, syringe-based assay systems that can be used to reproducibly assess induced innate or adaptive immune responses. By eliminating preanalytical errors associated with immune monitoring, we have defined the protein signatures induced by (1) medically relevant bacteria, fungi, and viruses; (2) agonists specific for defined host sensors; (3) clinically employed cytokines; and (4) activators of T cell immunity. Our results provide an initial assessment of healthy donor reference values for induced cytokines and chemokines and we report the failure to release interleukin-1α as a common immunological phenotype. The observed naturally occurring variation of the immune response may help to explain differential susceptibility to disease or response to therapeutic intervention. The implementation of a general solution for assessment of functional immune responses will help support harmonization of clinical studies and data sharing.
Copyright © 2014 Elsevier Inc. All rights reserved.
Source: PubMed