MAMI: a birth cohort focused on maternal-infant microbiota during early life

Izaskun García-Mantrana, Cristina Alcántara, Marta Selma-Royo, Alba Boix-Amorós, Majda Dzidic, Jose Gimeno-Alcañiz, Isabel Úbeda-Sansano, Ignacio Sorribes-Monrabal, Ramón Escuriet, Fernando Gil-Raga, Anna Parra-Llorca, Cecilia Martínez-Costa, María Carmen Collado, MAMI team, Maria Carmen Collado, Izaskun García-Mantrana, Cristina Alcántara, Jose Gimeno-Alcañiz, Marta Selma-Royo, Alba Boix-Amorós, Majda Dzidic, Christine Baüerl, Eva Villoldo, Carlos Zafra, Laura Olivares, Gaspar Pérez-Martínez, Alex Mira, Maria Desamparados Ferrer, Jacobo Martínez Santamaria, Andrea Ahicart, Máximo Vento, Anna Parra-Llorca, María Gormaz, María Cernada, Cecilia Martínez-Costa, Bibiana Bertúa-Ríos, Beatriz Padilla, Elena Crehuá-Gaudiza, Alba Peretó-Moll, Amparo Rodríguez García, Maria Dolores Soler Rico, Fernando Gil-Raga, Nuria Bixquert Martínez, Isabel Úbeda-Sansano, Irene Rausell Segarra, Jose Luis Tortajada Soriano, Ignacio Sorribes-Monrabal, Luis C Blesa-Baviera, Amelia Peris Vidal, Llanos Madrigal Hornos, Ramón Escuriet, Teresa Gonzalo Del Moral, Pepi Domínguez Cano, Marga Franch I Ferrer, Concha Delgado, Adela Atero, Izaskun García-Mantrana, Cristina Alcántara, Marta Selma-Royo, Alba Boix-Amorós, Majda Dzidic, Jose Gimeno-Alcañiz, Isabel Úbeda-Sansano, Ignacio Sorribes-Monrabal, Ramón Escuriet, Fernando Gil-Raga, Anna Parra-Llorca, Cecilia Martínez-Costa, María Carmen Collado, MAMI team, Maria Carmen Collado, Izaskun García-Mantrana, Cristina Alcántara, Jose Gimeno-Alcañiz, Marta Selma-Royo, Alba Boix-Amorós, Majda Dzidic, Christine Baüerl, Eva Villoldo, Carlos Zafra, Laura Olivares, Gaspar Pérez-Martínez, Alex Mira, Maria Desamparados Ferrer, Jacobo Martínez Santamaria, Andrea Ahicart, Máximo Vento, Anna Parra-Llorca, María Gormaz, María Cernada, Cecilia Martínez-Costa, Bibiana Bertúa-Ríos, Beatriz Padilla, Elena Crehuá-Gaudiza, Alba Peretó-Moll, Amparo Rodríguez García, Maria Dolores Soler Rico, Fernando Gil-Raga, Nuria Bixquert Martínez, Isabel Úbeda-Sansano, Irene Rausell Segarra, Jose Luis Tortajada Soriano, Ignacio Sorribes-Monrabal, Luis C Blesa-Baviera, Amelia Peris Vidal, Llanos Madrigal Hornos, Ramón Escuriet, Teresa Gonzalo Del Moral, Pepi Domínguez Cano, Marga Franch I Ferrer, Concha Delgado, Adela Atero

Abstract

Background: Early microbial colonization is a relevant aspect in human health. Altered microbial colonization patterns have been linked to an increased risk of non-communicable diseases (NCDs). Advances in understanding host-microbe interactions highlight the pivotal role of maternal microbiota on infant health programming. This birth cohort is aimed to characterize the maternal microbes transferred to neonates during the first 1000 days of life, as well as to identify the potential host and environmental factors, such as gestational age, mode of delivery, maternal/infant diet, and exposure to antibiotics, which affect early microbial colonization.

Methods: MAMI is a prospective mother-infant birth cohort in the Spanish-Mediterranean area. Mothers were enrolled at the end of pregnancy and families were follow-up during the first years of life. Maternal-infant biological samples were collected at several time points from birth to 24 months of life. Clinical and anthropometric characteristics and dietary information is available. Specific qPCR and 16S rRNA gene sequencing as well as short chain fatty acid (SCFAs) profile would be obtained. Multivariable models will be used to identy associations between microbiota and clinical and anthropometric data controlling for confounders. MAMI would contribute to a better understanding of the interaction between diet, microbiota and host response in early life health programming, enabling new applications in the field of personalized nutrition and medicine.

Trial registration: The study is registered on the ClinicalTrial.gov platform NCT03552939. (June 12, 2018).

Keywords: Antibiotics; Birth; Breast milk; Cohort study; Diet; Early nutrition; Growth; Lactation; Meconium; Microbiota; Neonates.

Conflict of interest statement

Ethics approval and consent to participate

The study protocol of the project "The power of maternal microbes on infant health (MAMI) with the registration number 2015/0024 was approved by the Ethics Committee of each hospital and health centres (Hospital Universitario y Politécnico La Fe, Hospital Clínico Universitario de Valencia, Atención Primaria Comunidad Valencia and CEIC-Parc de Salut MAR) as well as by the local ethics committee of Atención Primaria- Generalitat Valenciana (CEIC-APCV). Written informed consent is obtained from all the participants prior to enrolment in the study.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
Flow chart of sampling procedure
Fig. 2
Fig. 2
Influencing factors on microbiota development during early life

References

    1. Young VB. The role of the microbiome in human health and disease: an introduction for clinicians. BMJ. 2017;356:j83.
    1. Clemente Jose C., Ursell Luke K., Parfrey Laura Wegener, Knight Rob. The Impact of the Gut Microbiota on Human Health: An Integrative View. Cell. 2012;148(6):1258–1270. doi: 10.1016/j.cell.2012.01.035.
    1. Amarasekera M, Prescott SL, Palmer DJ. Nutrition in early life, immune-programming and allergies: the role of epigenetics. Asian Pacific J allergy Immunol. 2013;31:175–82.
    1. Koleva Petya, Bridgman Sarah, Kozyrskyj Anita. The Infant Gut Microbiome: Evidence for Obesity Risk and Dietary Intervention. Nutrients. 2015;7(4):2237–2260. doi: 10.3390/nu7042237.
    1. Dominguez-Bello M. G., Costello E. K., Contreras M., Magris M., Hidalgo G., Fierer N., Knight R. Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns. Proceedings of the National Academy of Sciences. 2010;107(26):11971–11975. doi: 10.1073/pnas.1002601107.
    1. Jakobsson Hedvig E, Abrahamsson Thomas R, Jenmalm Maria C, Harris Keith, Quince Christopher, Jernberg Cecilia, Björkstén Bengt, Engstrand Lars, Andersson Anders F. Decreased gut microbiota diversity, delayed Bacteroidetes colonisation and reduced Th1 responses in infants delivered by Caesarean section. Gut. 2013;63(4):559–566. doi: 10.1136/gutjnl-2012-303249.
    1. Salminen S. Influence of mode of delivery on gut microbiota composition in seven year old children. Gut. 2004;53(9):1388–1389. doi: 10.1136/gut.2004.041640.
    1. García-Mantrana Izaskun, Bertua Bibiana, Martínez-Costa Cecilia, Collado Maria Carmen. Perinatal nutrition: How to take care of the gut microbiota? Clinical Nutrition Experimental. 2016;6:3–16. doi: 10.1016/j.yclnex.2016.02.002.
    1. Toscano M, De Grandi R, Grossi E, Drago L. Role of the human breast milk-associated microbiota on the newborns’ immune system: a mini review. Front Microbiol. 2017;8:2100.
    1. Li R., Dee D., Li C.-M., Hoffman H. J., Grummer-Strawn L. M. Breastfeeding and Risk of Infections at 6 Years. PEDIATRICS. 2014;134(Supplement):S13–S20. doi: 10.1542/peds.2014-0646D.
    1. Lodge CJ, Tan DJ, Lau MXZ, Dai X, Tham R, Lowe AJ, Bowatte G, Allen KJ, Dharmage SC. Breastfeeding and asthma and allergies: a systematic review and meta-analysis. Acta Paediatrica. 2015;104:38–53. doi: 10.1111/apa.13132.
    1. Stuebe Alison M. Does breastfeeding prevent the metabolic syndrome, or does the metabolic syndrome prevent breastfeeding? Seminars in Perinatology. 2015;39(4):290–295. doi: 10.1053/j.semperi.2015.05.008.
    1. Kunz C., Rudloff S., Baier W., Klein N., Strobel S. OLIGOSACCHARIDES IN HUMAN MILK: Structural, Functional, and Metabolic Aspects. Annual Review of Nutrition. 2000;20(1):699–722. doi: 10.1146/annurev.nutr.20.1.699.
    1. Guaraldi F, Salvatori G. Effect of breast and formula feeding on gut microbiota shaping in newborns. Front Cell Infect Microbiol. 2012;2:94.
    1. Fernández-Ballart Joan D., Piñol Josep Lluís, Zazpe Itziar, Corella Dolores, Carrasco Paula, Toledo Estefanía, Perez-Bauer Manuel, Martínez-González Miguel Ángel, Salas-Salvadó Jorge, Martín-Moreno José M. Relative validity of a semi-quantitative food-frequency questionnaire in an elderly Mediterranean population of Spain. British Journal of Nutrition. 2010;103(12):1808–1816. doi: 10.1017/S0007114509993837.
    1. Martinez-Gonzalez M. A., Corella D., Salas-Salvado J., Ros E., Covas M. I., Fiol M., Warnberg J., Aros F., Ruiz-Gutierrez V., Lamuela-Raventos R. M., Lapetra J., Munoz M. A., Martinez J. A., Saez G., Serra-Majem L., Pinto X., Mitjavila M. T., Tur J. A., Portillo M. d. P., Estruch R. Cohort Profile: Design and methods of the PREDIMED study. International Journal of Epidemiology. 2010;41(2):377–385. doi: 10.1093/ije/dyq250.
    1. Boix-Amorós A, Collado MC, Mira A. Relationship between Milk microbiota, bacterial load, macronutrients, and human cells during lactation. Front Microbiol. 2016;7:492. doi: 10.3389/fmicb.2016.00492.
    1. Collado MC, Isolauri E, Laitinen K, Salminen S. Effect of mother’s weight on infant's microbiota acquisition, composition, and activity during early infancy: a prospective follow-up study initiated in early pregnancy. Am J Clin Nutr. 2010;92(5):1023–1030. doi: 10.3945/ajcn.2010.29877.
    1. Sarmiento-Rubiano Luz Adriana, Zúñiga Manuel, Pérez-Martínez Gaspar, Yebra María Jesús. Dietary supplementation with sorbitol results in selective enrichment of lactobacilli in rat intestine. Research in Microbiology. 2007;158(8-9):694–701. doi: 10.1016/j.resmic.2007.07.007.
    1. Kunz Clemens, Meyer Christina, Collado Maria Carmen, Geiger Lena, García-Mantrana Izaskun, Bertua-Ríos Bibiana, Martínez-Costa Cecilia, Borsch Christian, Rudloff Silvia. Influence of Gestational Age, Secretor, and Lewis Blood Group Status on the Oligosaccharide Content of Human Milk. Journal of Pediatric Gastroenterology and Nutrition. 2017;64(5):789–798. doi: 10.1097/MPG.0000000000001402.
    1. Sprenger Norbert, Lee Le Ye, De Castro Carlos Antonio, Steenhout Philippe, Thakkar Sagar K. Longitudinal change of selected human milk oligosaccharides and association to infants’ growth, an observatory, single center, longitudinal cohort study. PLOS ONE. 2017;12(2):e0171814. doi: 10.1371/journal.pone.0171814.
    1. Langille MG, Zaneveld J, Caporaso JG, McDonald D, Knights D, Reyes JA, Clemente JC, Burkepile DE, Vega Thurber RL, Knight R, Beiko RG, Huttenhower C. Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. Nat Biotechnol. 2013;31:814–821. doi: 10.1038/nbt.2676.
    1. Segata N, Izard J, Waldron L, Gevers D, Miropolsky L, Garrett WS, Huttenhower C. Metagenomic biomarker discovery and explanation. Genome Biol. 2011;12(6):R60. doi: 10.1186/gb-2011-12-6-r60.

Source: PubMed

Sponsors and Collaborators

Medical Conditions

Drug Interventions

3
Subscribe