Apelin-13 Administration Protects Against LPS-Induced Acute Lung Injury by Inhibiting NF-κB Pathway and NLRP3 Inflammasome Activation
Hailin Zhang, Sha Chen, Meichun Zeng, Daopeng Lin, Yu Wang, Xunhang Wen, Changfu Xu, Li Yang, Xiaofang Fan, Yongsheng Gong, Hongyu Zhang, Xiaoxia Kong, Hailin Zhang, Sha Chen, Meichun Zeng, Daopeng Lin, Yu Wang, Xunhang Wen, Changfu Xu, Li Yang, Xiaofang Fan, Yongsheng Gong, Hongyu Zhang, Xiaoxia Kong
Abstract
Background/aims: Acute lung injury (ALI) is induced by a variety of external and internal factors and leads to acute progressive respiratory failure. Previous studies have shown that apelin-13 can decrease the acute lung injury induced by LPS, but the specific mechanism is unclear. Therefore, a mouse lung injury model and a cell model were designed to explore the mechanism of how apelin-13 alleviates the acute lung injury caused by LPS.
Methods: The effect of apelin-13 on LPS-induced structural damage was determined by H&E staining and by the wet/dry weight ratio. The related inflammatory factors in BALF were examined by ELISA. The apoptotic pathway and the NF-κB and NLRP3 inflammasome pathways were evaluated by using Western blotting and immunofluorescence staining.
Results: LPS induced the structural damage and the production of inflammatory cytokines in the lung tissues of mice. These deleterious effects were attenuated by apelin-13 administration. The protective effects of apelin-13 were associated with decreased reactive oxygen species (ROS) formation and the inhibition of the activation of the NF-κB and NLRP3 inflammasome pathways in mice and in Raw264.7 cells.
Conclusion: Taken together, these data suggest that apelin-13 administration ameliorates LPS-induced acute lung injury by suppressing ROS formation, as well as by inhibiting the NF-κB pathway and the activation of the NLRP3 inflammasome in the lungs.
Keywords: Acute lung injury (ALI); Apelin-13; Lipopolysaccharide (LPS); NF-κB; NLRP3.
© 2018 The Author(s). Published by S. Karger AG, Basel.
Source: PubMed