Sickle Cell Disease Biofluid Chip Technology (SCD BioChip)
9. september 2026 opdateret af: AOwusu-Ansah, University Hospitals Cleveland Medical Center
Sickle Cell Disease (SCD) Biochip': Mod en enkel og pålidelig måde at overvåge seglcellesygdom på
'Seglformet' anæmi blev første gang klinisk beskrevet i USA i 1910, og det muterede arvelige seglhæmoglobinmolekyle blev identificeret i 1949. Patofysiologien af SCD er en konsekvens af unormal polymerisation af seglhæmoglobin (HbS) og dets virkninger på røde blodlegemers membranegenskaber, form og tæthed og efterfølgende kritiske ændringer i inflammatorisk celle- og endotelcellefunktion. Vores mål er at forstå virkningen af CMA-abnormiteter i SCD ved at undersøge en række anerkendte interaktioner i en række kliniske fænotyper.
Til dato har korrelative undersøgelser i SCD, af os og andre, spændet mellem kliniske rapporter baseret på tests, interventioner og diagramgennemgang af individer eller grupper af individer og i den anden yderlighed identifikation af funktionelle genpolymorfismer baseret på befolkningsundersøgelser . Forskerne ønsker at udvide disse undersøgelser gennem en systematisk undersøgelse af cellulære membranegenskaber og aktiveringsstatus. Af hæmatologiske lidelser kan SCD være usædvanligt modtagelig for en sådan undersøgelse.
Studieoversigt
Detaljeret beskrivelse
Ny biofluidisk chipteknologi kan undersøge overfladekarakteristika, der typisk måles med konventionelle teknikker, såsom fluorescerende aktiveret cellesortering (FACS), immunhistokemi eller mikroskopiske billeddannelsesmetoder.
I FACS isoleres celler af interesse, behandles omfattende, inkuberes med et fluorescensmærket antistof og sorteres ved optisk genkendelse.
I den foreslåede SCD-biofluidchip (SCD-biochip) dækker det forespørgende antistof mikrokanaloverfladen og fanger cellepopulationen(e) af interesse uden behandling, inkubation eller in vitro-manipulation.
SCD-biochippen kan også kvantificere cellulær adhærens til eksperimentelle biologiske overflader, der består af subcellulære komponenter.
SCD-biochippen er teknisk enkel og eksperimentelt fleksibel, hvorved populationen af interesse fastholdes på chippen og kvantificeres in situ.
Mikrochipsystemet tillader genfinding af levedygtige isolerede celler til potentiel downstream-behandling, analyse og in vitro-kultur.
Undersøgelsestype
Observationel
Tilmelding (Anslået)
100
Kontakter og lokationer
Dette afsnit indeholder kontaktoplysninger for dem, der udfører undersøgelsen, og oplysninger om, hvor denne undersøgelse udføres.
Studiekontakt
- Navn: Umut Gurkan, PhD
- Telefonnummer: (216) 368-6447
- E-mail: umut@case.edu
Studiesteder
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Ohio
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Cleveland, Ohio, Forenede Stater, 44106
- Rekruttering
- University Hospitals Case Medical Center
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Kontakt:
- Umut A Gurkan, Ph.D.
- Telefonnummer: 216-368-6447
- E-mail: umut@case.edu
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Ledende efterforsker:
- Amma Owusu-Ansah, MD
-
Kontakt:
- Amma Owusu-Ansah, MD
- Telefonnummer: 216-844-3345
- E-mail: Amma.Owusu-Ansah@UHhospitals.org
-
-
Deltagelseskriterier
Forskere leder efter personer, der passer til en bestemt beskrivelse, kaldet berettigelseskriterier. Nogle eksempler på disse kriterier er en persons generelle helbredstilstand eller tidligere behandlinger.
Berettigelseskriterier
Aldre berettiget til at studere
12 år og ældre (Barn, Voksen, Ældre voksen)
Tager imod sunde frivillige
Ja
Prøveudtagningsmetode
Ikke-sandsynlighedsprøve
Studiebefolkning
Børn (≥12 år) og voksne med SCD, inklusive HbSS eller sammensat heterozygus HbSC- eller HbSβ-thalassæmi vil blive tilbudt deltagelse.
Kontrolprøver (HbAA) kan fås fra raske frivillige.
Beskrivelse
Inklusionskriterier
- Mand eller kvinde ≥12 år på tidspunktet for samtykke (tilmelding).
- Dokumentation seglcellesygdom, herunder HbSS eller sammensat heterozygus HbSC- eller HbSβ-thalassæmi-diagnose som påvist af et eller flere kliniske træk.
- Skriftligt informeret samtykke (og samtykke, når det er relevant) opnået fra forsøgspersonens eller forsøgspersonens juridiske repræsentant og forsøgspersonens evne til at overholde kravene i undersøgelsen.
Eksklusionskriterier
- Tilstedeværelse af en tilstand eller abnormitet, som efter investigatorens mening ville kompromittere patientens sikkerhed eller kvaliteten af dataene.
Studieplan
Dette afsnit indeholder detaljer om studieplanen, herunder hvordan undersøgelsen er designet, og hvad undersøgelsen måler.
Hvordan er undersøgelsen tilrettelagt?
Design detaljer
- Observationsmodeller: Case-Control
- Tidsperspektiver: Fremadrettet
Kohorter og interventioner
Gruppe / kohorte |
Intervention / Behandling |
|---|---|
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Mindre SCD-gruppe, 12-17 år
Ingen indgriben.
Kun brug af kasseret blod/væv
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Ingen indgriben.
Brug af kasseret blod/væv
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Voksen SCD. Alder 18+
Ingen indgriben.
Kun brug af kasseret blod/væv
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Ingen indgriben.
Brug af kasseret blod/væv
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Hvad måler undersøgelsen?
Primære resultatmål
Resultatmål |
Foranstaltningsbeskrivelse |
Tidsramme |
|---|---|---|
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Udvikle en SCD-biochip til at undersøge centrale cellulære egenskaber og interaktioner, herunder RBC og WBC cellulære, adhæsive og inflammatoriske egenskaber og cirkulerende endotel- og hæmatopoietiske precursorcellekarakteristika.
Tidsramme: 2 år
|
Røde og hvide blodlegemers adhæsion til biomolekyler (f.
laminin, fibronectin og selectiner) vil blive målt på en mikrofluidisk enhed, SCD Biochip.
Adhæsion vil blive målt under normale ilt- og laviltforhold.
Adhæsioner vil blive vurderet i forhold til kliniske fund, såsom hæmatologiske parametre, og evidens for vaso-okklusion, smerte, inflammation og vaskulopati hos patienter med seglcellesygdom.endotelial
og hæmatopoietiske precursorceller baseret på membranegenskaber og adhæsion.
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2 år
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Sekundære resultatmål
Resultatmål |
Foranstaltningsbeskrivelse |
Tidsramme |
|---|---|---|
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Korreler SCD Biochip-funktion i heterogene SCD-populationer, inklusive HbSS og HbSC i en række aldre, og i dem med akutte og kroniske komplikationer og sammenlignet med normale kontroller.
Tidsramme: 2 år
|
Efterforskerne vil undersøge og validere kliniske korrelationer med disse cellulære/membranegenskaber i større populationer af SCD på tværs af en række fænotyper ved hjælp af vores enkle hurtige fleksible SCD Biochip-platform.
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2 år
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Samarbejdspartnere og efterforskere
Det er her, du vil finde personer og organisationer, der er involveret i denne undersøgelse.
Samarbejdspartnere
Efterforskere
- Ledende efterforsker: Amma Owusu-Ansah, MD, University Hospitals Cleveland Medical Center
Publikationer og nyttige links
Den person, der er ansvarlig for at indtaste oplysninger om undersøgelsen, leverer frivilligt disse publikationer. Disse kan handle om alt relateret til undersøgelsen.
Generelle publikationer
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Datoer for undersøgelser
Disse datoer sporer fremskridtene for indsendelser af undersøgelsesrekord og resumeresultater til ClinicalTrials.gov. Studieregistreringer og rapporterede resultater gennemgås af National Library of Medicine (NLM) for at sikre, at de opfylder specifikke kvalitetskontrolstandarder, før de offentliggøres på den offentlige hjemmeside.
Studer store datoer
Studiestart (Faktiske)
1. oktober 2014
Primær færdiggørelse (Anslået)
31. maj 2030
Studieafslutning (Anslået)
31. maj 2030
Datoer for studieregistrering
Først indsendt
27. maj 2015
Først indsendt, der opfyldte QC-kriterier
5. juli 2016
Først opslået (Anslået)
6. juli 2016
Opdateringer af undersøgelsesjournaler
Sidste opdatering sendt (Faktiske)
11. september 2026
Sidste opdatering indsendt, der opfyldte kvalitetskontrolkriterier
9. september 2026
Sidst verificeret
1. september 2026
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