Ikke-invasiv hjernetumor molekylær diagnostik og overvågning
Ikke-invasiv hjernetumordiagnose gennem analyse af proksimale væsker, cirkulerende tumorceller og biomolekyler
This prospective multicenter study aims to enroll GBM patients who will undergo repeated assessments (preoperatively and 3 months post-surgery) to detect circulating tumor cells and analyze the transcriptomic profiles of EVs in their blood. The prognostic and monitoring significance of these biomarkers to disease course (assessing treatment efficacy, resistance incidence, tumor progression) will be evaluated.
Concurrently, proteomic profiles typical of GBM will be analyzed in blood and ocular secretion samples from GBM patients, patients with low-grade gliomas, and patients without brain tumors to identify and validate novel protein biomarkers suitable for disease monitoring. Additionally, this study proposes an innovative approach to monitor GBM patients by investigating the presence of GBM-specific nucleic acid fragments in urine.
Studieoversigt
Status
Betingelser
Intervention / Behandling
Undersøgelsestype
Tilmelding (Anslået)
Kontakter og lokationer
Studiekontakt
- Navn: Josef Srovnal, MD,PhD
- Telefonnummer: +420585632137
- E-mail: josef.srovnal@upol.cz
Undersøgelse Kontakt Backup
- Navn: Ondrej Kalita, MD,PhD
- Telefonnummer: +420588443568
- E-mail: ondrej.kalita@fnol.cz
Studiesteder
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Olomouc, Tjekkiet, 77900
- Rekruttering
- Department of Neurosurgery, University Hospital Olomouc
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Kontakt:
- Ondrej Kalita, MD, PhD
- Telefonnummer: +420588443568
- E-mail: ondrej.kalita@fnol.cz
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Deltagelseskriterier
Berettigelseskriterier
Aldre berettiget til at studere
Tager imod sunde frivillige
Køn, der er berettiget til at studere
Prøveudtagningsmetode
Studiebefolkning
Inhabitants of Olomouc region
Beskrivelse
Inclusion Criteria: Adult patients with brain tumors -
Exclusion Criteria: No other cancer; no inflammatory disease
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Studieplan
Hvordan er undersøgelsen tilrettelagt?
Design detaljer
- Observationsmodeller: CASE_CONTROL
- Tidsperspektiver: PROSPECTIVE
Kohorter og interventioner
Gruppe / kohorte |
Intervention / Behandling |
|---|---|
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Glioblastoma multiforme
Patients with GBM |
No intervention among the arms. The diagnostic, prognostic, predictive and follow-up value of tested biomolecules will be analyzed. |
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Low-grade glioma
Patients with low-grade gliomas. |
No intervention among the arms. The diagnostic, prognostic, predictive and follow-up value of tested biomolecules will be analyzed. |
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Control group
Patients without cancer or systemic inflammation |
No intervention among the arms. The diagnostic, prognostic, predictive and follow-up value of tested biomolecules will be analyzed. |
Hvad måler undersøgelsen?
Primære resultatmål
Resultatmål |
Foranstaltningsbeskrivelse |
Tidsramme |
|---|---|---|
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Proteomic profiles of proximal liquids in brain tumor patients
Tidsramme: 2020-2023
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The protein profiles will be measured in ocular secretion and plasma samples of patients wiht GBM, low-grade glioma and control cohort. Candidate proteins will be correleted with clinical parameters. The number of patients with identified prognostic biomarkers will be reported. |
2020-2023
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Circulating tumor cells (CTCs) for non-invasive monitoring of GBM patients.
Tidsramme: 2020-2030
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The number of CTCs and their molecular characteristics will be measured in peripheral blod of GBM patients. The CTCs counts will be correleted with the clinical parameters. The number of patients with the presence or absence of CTCs will be reported. |
2020-2030
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Extracellular vesicles (EVs) for non-invasive monitoring of GBM patients.
Tidsramme: 2020-2030
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The RNA profiles of EVs will be measured in plasma samples of patients wiht GBM. Candidate RNAs will be correleted with clinical parameters. The number of patients with identified prognostic RNAs will be reported. |
2020-2030
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Urine as a source of GBM-specific nucleic acids fragments for disease monitoring.
Tidsramme: 2025-2030
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The GBM-specific nucleic acids fragments will be measured in urine of GBM patients. Candidate nucleic acids will be correleted with clinical parameters. The number of patients with identified prognostic biomolecules will be reported. |
2025-2030
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Samarbejdspartnere og efterforskere
Efterforskere
- Ledende efterforsker: Marian Hajduch, MD,PhD, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic
Datoer for undersøgelser
Studer store datoer
Studiestart (Faktiske)
Primær færdiggørelse (Anslået)
Studieafslutning (Anslået)
Datoer for studieregistrering
Først indsendt
Først indsendt, der opfyldte QC-kriterier
Først opslået (Faktiske)
Opdateringer af undersøgelsesjournaler
Sidste opdatering sendt (Faktiske)
Sidste opdatering indsendt, der opfyldte kvalitetskontrolkriterier
Sidst verificeret
Mere information
Begreber relateret til denne undersøgelse
Yderligere relevante MeSH-vilkår
Andre undersøgelses-id-numre
- NW25-03-00198
Plan for individuelle deltagerdata (IPD)
Planlægger du at dele individuelle deltagerdata (IPD)?
IPD-planbeskrivelse
Anonymous proteomic profiles of plasma and ocular secretion and transcriptomic data from extracelular vesicles will be shared.
Lægemiddel- og udstyrsoplysninger, undersøgelsesdokumenter
Studerer et amerikansk FDA-reguleret lægemiddelprodukt
Studerer et amerikansk FDA-reguleret enhedsprodukt
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