Chimeric Antigen Receptor T Cells (CART) Therapy in Refractory/Relapsed B Cell Hematologic Malignancies

Inclusion Criteria:

• Patients diagnosed as CD19 positive refractory and relapsed B cell hematologic malignancies included acute lymphoblastic leukemia (ALL),chronic lymphocytic leukemia(CLL),non-Hodgkin's lymphoma(NHL)
• not eligible or appropriate for auto-HSCT or allo-HSCT or relapsed after hematopoietic stem cell transplant(HSCT)
• At least one measurable lesion defined as one lesion larger than 1.5cm or two lesion more than 1.0cm(for patients with NHL )
• Age ≤60 years
• Eastern Cooperative Oncology Group(ECOG) Performance status 0 to 2, Expected survival > 6 months
• Left Ventricular Ejection Fraction (LVEF) > 50%
• no history of other malignancies;
• no other serious diseases which conflict with the treatment in the present trial
• All patients should consent to adopt efficient contraception methods during the treatment and after the treatment. The pregnant tests of women who are in child bearing period should be negative before the treatment.
• patients should understand and are willing to participate in the trial. Inform consent form is supposed to obtained before treatment

Exclusion Criteria:

• Diagnosis or classification undefined
• Those with primary central nervous system lymphoma or testicular leukaemia or lymphoma
• Patients with a known history or prior diagnosis of epilepsia or other disease affecting the central nervous system, or serious mental diseases;
• Patients who have secondary leukaemia or lymphoma after chemotherapy or radiotherapy for other malignancies
• Active acute or chronic graft-versus-host disease (GVHD) requiring systemic therapy, concurrent use of immunosuppressant medications
• Class III/IV cardiovascular disability according to the New York Heart Association Classification
• Pregnant or lactating women(the safety of this therapy on unborn children is not known)
• With active infection
• Active hepatitis B, hepatitis C or syphilis infection
• Patients use of systemic steroids within 2 weeks. Recent or current use of inhaled steroids is not exclusionary
• Prior treatment with gene therapy product
• Cluster of differentiation 3(CD3) positive cells<0.9x10^4/ml in peripheral blood (PB)
• Organ function meeting following criteria: liver and renal function: alanine aminotransferase(ALT)/aspartate aminotransferase(AST) > 3 times the upper limit of normal, or bilirubin>2.0 mg/dl（34.2umol/L）, or creatinine >2.5mg/dl（221.0umol/L） ; hematopoietic function:Neutrophil count<1.0x10^9/L，hemoglobin<80g/L，platelet <50x10^9/L(for patients with NHL )
• Any uncontrolled active medical disorder that would preclude participation as outlined
• HIV infection
• The researchers considered unsuitable to participate in this clinical study.
• Patients with poor compliance