Growth-inhibitory effects of a mineralized extract from the red marine algae, Lithothamnion calcareum, on Ca(2+)-sensitive and Ca(2+)-resistant human colon carcinoma cells
Muhammad Nadeem Aslam, Narasimharao Bhagavathula, Tejaswi Paruchuri, Xin Hu, Subhas Chakrabarty, James Varani, Muhammad Nadeem Aslam, Narasimharao Bhagavathula, Tejaswi Paruchuri, Xin Hu, Subhas Chakrabarty, James Varani
Abstract
Proliferation and differentiation were assessed in a series of human colon carcinoma cell lines in response to a mineral-rich extract derived from the red marine algae, Lithothamnion calcareum. The extract contains 12% Ca2+, 1% Mg2+, and detectable amounts of 72 trace elements, but essentially no organic material. The red algae extract was as effective as inorganic Ca2+ alone in suppressing growth and inducing differentiation of colon carcinoma cells that are responsive to a physiological level of extracellular Ca2+ (1.4mM). However, with cells that are resistant to Ca2+ alone, the extract was still able to reduce proliferation and stimulate differentiation.
Conflict of interest statement
Conflicts of Interest Statement
All named authors of the original research paper entitled “Growth-inhibitory effects of a mineralized extract from the red marine algae, Lithothamnion calcareum, on Ca2+-sensitive and Ca2+-resistant human colon carcinoma cells” express that there exist no financial and/or personal relationships with other people and/or organizations that could inappropriately influence their work.
Figures
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Fig. 3
Effects of a red algae…
Fig. 3
Effects of a red algae extract on growth of four different human colon…
- Human colon tissue in organ culture: calcium and multi-mineral-induced mucosal differentiation.Dame MK, Veerapaneni I, Bhagavathula N, Naik M, Varani J. Dame MK, et al. In Vitro Cell Dev Biol Anim. 2011 Jan;47(1):32-8. doi: 10.1007/s11626-010-9358-3. Epub 2010 Nov 20. In Vitro Cell Dev Biol Anim. 2011. PMID: 21104039 Free PMC article.
- A mineral-rich red algae extract inhibits polyp formation and inflammation in the gastrointestinal tract of mice on a high-fat diet.Aslam MN, Paruchuri T, Bhagavathula N, Varani J. Aslam MN, et al. Integr Cancer Ther. 2010 Mar;9(1):93-9. doi: 10.1177/1534735409360360. Epub 2010 Feb 11. Integr Cancer Ther. 2010. PMID: 20150219 Free PMC article.
- A mineral-rich extract from the red marine algae Lithothamnion calcareum preserves bone structure and function in female mice on a Western-style diet.Aslam MN, Kreider JM, Paruchuri T, Bhagavathula N, DaSilva M, Zernicke RF, Goldstein SA, Varani J. Aslam MN, et al. Calcif Tissue Int. 2010 Apr;86(4):313-24. doi: 10.1007/s00223-010-9340-9. Epub 2010 Feb 24. Calcif Tissue Int. 2010. PMID: 20180099 Free PMC article.
- Anticancer compounds and sphingolipid metabolism in the colon.Duan RD. Duan RD. In Vivo. 2005 Jan-Feb;19(1):293-300. In Vivo. 2005. PMID: 15796189 Review.
- A role for Hath1, a bHLH transcription factor, in colon adenocarcinoma.Leow CC, Polakis P, Gao WQ. Leow CC, et al. Ann N Y Acad Sci. 2005 Nov;1059:174-83. doi: 10.1196/annals.1339.048. Ann N Y Acad Sci. 2005. PMID: 16382053 Review.
- A Multi-Mineral Intervention to Modulate Colonic Mucosal Protein Profile: Results from a 90-Day Trial in Human Subjects.Aslam MN, McClintock SD, Jawad-Makki MAH, Knuver K, Ahmad HM, Basrur V, Bergin IL, Zick SM, Sen A, Turgeon DK, Varani J. Aslam MN, et al. Nutrients. 2021 Mar 14;13(3):939. doi: 10.3390/nu13030939. Nutrients. 2021. PMID: 33799486 Free PMC article. Clinical Trial.
- A Calcium-Rich Multimineral Intervention to Modulate Colonic Microbial Communities and Metabolomic Profiles in Humans: Results from a 90-Day Trial.Aslam MN, Bassis CM, Bergin IL, Knuver K, Zick SM, Sen A, Turgeon DK, Varani J. Aslam MN, et al. Cancer Prev Res (Phila). 2020 Jan;13(1):101-116. doi: 10.1158/1940-6207.CAPR-19-0325. Epub 2019 Nov 26. Cancer Prev Res (Phila). 2020. PMID: 31771942 Free PMC article. Clinical Trial.
- Calcium-induced differentiation in normal human colonoid cultures: Cell-cell / cell-matrix adhesion, barrier formation and tissue integrity.Attili D, McClintock SD, Rizvi AH, Pandya S, Rehman H, Nadeem DM, Richter A, Thomas D, Dame MK, Turgeon DK, Varani J, Aslam MN. Attili D, et al. PLoS One. 2019 Apr 17;14(4):e0215122. doi: 10.1371/journal.pone.0215122. eCollection 2019. PLoS One. 2019. PMID: 30995271 Free PMC article.
- Seaweed Extract (Stella Maris®) Activates Innate Immune Responses in Arabidopsis thaliana and Protects Host against Bacterial Pathogens.Cook J, Zhang J, Norrie J, Blal B, Cheng Z. Cook J, et al. Mar Drugs. 2018 Jun 28;16(7):221. doi: 10.3390/md16070221. Mar Drugs. 2018. PMID: 29958402 Free PMC article.
- Calcium-Induced Differentiation of Human Colon Adenomas in Colonoid Culture: Calcium Alone versus Calcium with Additional Trace Elements.McClintock SD, Colacino JA, Attili D, Dame MK, Richter A, Reddy AR, Basrur V, Rizvi AH, Turgeon DK, Varani J, Aslam MN. McClintock SD, et al. Cancer Prev Res (Phila). 2018 Jul;11(7):413-428. doi: 10.1158/1940-6207.CAPR-17-0308. Epub 2018 Apr 10. Cancer Prev Res (Phila). 2018. PMID: 29636350 Free PMC article.
- Adenocarcinoma / metabolism*
- Apoptosis / drug effects
- Blotting, Western
- Cell Differentiation / drug effects
- Cell Line, Tumor
- Cell Proliferation / drug effects*
- Colonic Neoplasms / metabolism*
- Complex Mixtures / pharmacology*
- Fluorescent Antibody Technique
- Growth Inhibitors / pharmacology
- Humans
- Microscopy, Confocal
- Rhodophyta / chemistry*
- Complex Mixtures
- Growth Inhibitors
- Full Text Sources
- Other Literature Sources
- Medical
- Miscellaneous
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Source: PubMed