Evaluation of antiproliferative activity of red sorghum bran anthocyanin on a human breast cancer cell line (mcf-7)

P Suganya Devi, M Saravana Kumar, S Mohan Das, P Suganya Devi, M Saravana Kumar, S Mohan Das

Abstract

Breast cancer is a leading cause of death in women worldwide both in the developed and developing countries. Thus effective treatment of breast cancer with potential antitumour drugs is important. In this paper, human breast cancer cell line MCF-7 has been employed to evaluate the antiproliferative activity of red sorghum bran anthocyanin. The present investigation showed that red sorghum bran anthocyanin induced growth inhibition of MCF-7 cells at significant level. The growth inhibition is dose dependent and irreversible in nature. When MCF-7 cells were treated with red sorghum bran anthocyanins due to activity of anthocyanin morphological changes were observed. The morphological changes were identified through the formation of apoptopic bodies. The fragmentation by these anthocyanins on DNA to oligonuleosomal-sized fragments, is a characteristic of apoptosis, and it was observed as concentration-dependent. Thus, this paper clearly demonstrates that human breast cancer cell MCF-7 is highly responsive by red sorghum bran anthocyanins result from the induction of apoptosis in MCF-7 cells.

Figures

Figure 1
Figure 1
(a) Normal MCF-7 cells. (b) Anthocyanin treated MCF-7 cells showing spherical shaped cells leading to loss of cell anchorage with concentration of 1000 μg/mL. (c) Anthocyanin treated MCF-7 cells showing spherical shaped cells leading to loss of cell anchorage with concentration of 500 μg/mL. (d) Anthocyanin treated MCF-7 cells showing spherical shaped cells leading to loss of cell anchorage and cell number was also reduced with concentration of 250 μg/mL.
Figure 2
Figure 2
Relative cell viability of MCF-7 cells at different concentration of anthocyanin extracted by methanol.
Figure 3
Figure 3
Relative cell viability of MCF-7 cells at different concentration of anthocaynin extracted by acidified methanol.
Figure 4
Figure 4
The DNA fragmentation analysis of oligonuceosomalsized fragments in the MCF-7 cells treated with red sorghum bran anthocyanins.

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Source: PubMed

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