Effect of Differently Fed Farmed Gilthead Sea Bream Consumption on Platelet Aggregation and Circulating Haemostatic Markers among Apparently Healthy Adults: A Double-Blind Randomized Crossover Trial

Agathi Ntzouvani, Smaragdi Antonopoulou, Elizabeth Fragopoulou, Meropi D Kontogianni, Tzortzis Nomikos, Anastasia Mikellidi, Μarianna Xanthopoulou, Nick Kalogeropoulos, Demosthenes Panagiotakos, Agathi Ntzouvani, Smaragdi Antonopoulou, Elizabeth Fragopoulou, Meropi D Kontogianni, Tzortzis Nomikos, Anastasia Mikellidi, Μarianna Xanthopoulou, Nick Kalogeropoulos, Demosthenes Panagiotakos

Abstract

Fish consumption beneficially affects coagulation markers. Few dietary intervention studies have investigated differently fed farmed fish against these cardio-metabolic risk factors in humans. This double-blind randomized crossover trial evaluated differently fed farmed gilthead sea bream consumption against platelet aggregation and circulating haemostatic markers among apparently healthy adults. Subjects aged 30-65 years, with a body mass index 24.0-31.0 kg/m2, consuming less than 150 g cooked fish per week, were recruited in Attica, Greece. Participants were randomized (n = 38, 1:1) to one of two sequences; consumption of fish fed with fish oil diet (conventional fish, CF)/fish fed with olive pomace-enriched diet (enriched fish, EF) versus EF/CF. The primary outcomes were ex vivo human platelet aggregation and circulating plasminogen activator inhibitor-1 (PAI-1) and P-selectin (sP-selectin) concentrations. EF consumption had no significant effect on platelet sensitivity or haemostatic markers compared to CF. Platelet sensitivity to platelet-activating factor (PAF) decreased after CF consumption during the second period (p < 0.01). Plasma PAI-1 and sP-selectin concentrations increased after CF consumption during both periods (p < 0.01 for both). Based on current findings, consumption of enriched farmed gilthead sea bream had no greater effect on coagulation markers in adults compared to the conventionally fed fish.

Keywords: ADP; PAF; PAI-1; fish; human platelet aggregation; olive pomace; sP-selectin; thrombin.

Conflict of interest statement

S. Antonopoulou holds a World patent for the production method of fish feed enriched with polar lipids and method to enrich farmed fishes (WO 2014/122571 A1. 2014 August 14). All other authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

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