Serum IL-1beta, IL-6, IL-8, and TNF-alpha levels in early diagnosis and management of neonatal sepsis

A Nese Citak Kurt, A Denizmen Aygun, Ahmet Godekmerdan, Abdullah Kurt, Yasar Dogan, Erdal Yilmaz, A Nese Citak Kurt, A Denizmen Aygun, Ahmet Godekmerdan, Abdullah Kurt, Yasar Dogan, Erdal Yilmaz

Abstract

Aim: To determine serum IL-1beta, IL-6, IL-8, and TNF-alpha levels in neonatal sepsis at the time of diagnosis and after therapy, and to show the meaningful on the follow up.

Methods: This prospective study was performed on newborns who were hospitalized for neonatal sepsis and who were classified as culture-proven sepsis (n=12), as culture-negative sepsis (n=21), and as healthy newborns (n=17).

Results: At the time of diagnosis, serum IL-1beta, IL-6, IL-8, and TNF-alpha levels of culture-proven sepsis were significantly higher than those of the control groups (P<.05). At the time of diagnosis, IL-1beta, IL-6, IL-8, and TNF-alpha levels of culture-proven sepsis and culture-negative sepsis were significantly higher than levels at the seventh day after antibiotic treatment.

Conclusion: Serum IL-1beta, IL-6, IL-8, and TNF-alpha are mediators of inflammation and can be used at the diagnosis and at the evaluation of the therapeutic efficiency in neonatal sepsis.

References

    1. Volante E, Moretti S, Pisani F, Bevilacqua G. Early diagnosis of bacterial infection in the neonate. Journal of Maternal-Fetal & Neonatal Medicine. 2004;16(2):13–16.
    1. Gladstone IM, Ehrenkranz RA, Edberg SC, Baltimore RS. A ten-year review of neonatal sepsis and comparison with the previous fifty-year experience. Pediatric Infectious Disease Journal. 1990;9(11):819–825.
    1. Vesikari R, Janas M, Grönroos P, et al. Neonatal septicaemia. Archives of Disease in Childhood. 1985;60(6):542–546.
    1. Kite P, Millar MR, Gorham P, Congdon P. Comparison of five tests used in diagnosis of neonatal bacteraemia. Archives of Disease in Childhood. 1988;63(6):639–643.
    1. Ainbender E, Cabatu EE, Guzman DM, Sweet AY. Serum C-reactive protein and problems of newborn infants. Journal of Pediatrics. 1982;101(3):438–440.
    1. Da Silva O, Ohlsson A, Kenyon C. Accuracy of leukocyte indices and C-reactive protein for diagnosis of neonatal sepsis: a critical review. Pediatric Infectious Disease Journal. 1995;14(5):1119–1120.
    1. Manroe BL, Weinberg AG, Rosenfeld CR, Browne R. The neonatal blood count in health and disease. I. Reference values for neutrophilic cells. Journal of Pediatrics. 1979;95(1):89–98.
    1. Zipursky A, Palko J, Milner R, Akenzua GI. The hematology of bacterial infections in premature infants. Pediatrics. 1976;57(6):839–853.
    1. Mathers NJ, Pohlandt F. Diagnostic audit of C-reactive protein in neonatal infection. European Journal of Pediatrics. 1987;146(2):147–151.
    1. Sann L, Bienvenu F, Bienvenu J. Evolution of serum prealbumin, C-reactive protein, and orosomucoid in neonates with bacterial infection. Journal of Pediatrics. 1984;105(6):977–981.
    1. Berner R, Niemeyer CM, Leititis JU, et al. Plasma levels and gene expression of granulocyte colony-stimulating factor, tumor necrosis factor-α, interleukin (IL)-1β, IL-6, IL-8, and soluble intercellular adhesion molecule-1 in neonatal early onset sepsis. Pediatric Research. 1998;44(4):469–477.
    1. Cairo MS. Neonatal neutrophil host defense. Prospects for immunologic enhancement during neonatal sepsis. American Journal of Diseases of Children. 1989;143(1):40–46.
    1. Buck C, Bundschu J, Gallati H, Bartmann P, Pohlandt F. Interleukin-6: a sensitive parameter for the early diagnosis of neonatal bacterial infection. Pediatrics. 1994;93(1):54–58.
    1. de Bont ES, Martens A, van Raan J, et al. Diagnostic value of plasma levels of tumor necrosis factor α (TNFα) and interleukin-6 (IL-6) in newborns with sepsis. Acta Paediatrica. 1994;83(7):696–699.
    1. Harris MC, Costarino AT, Jr., Sullivan JS, et al. Cytokine elevations in critically ill infants with sepsis and necrotizing enterocolitis. Journal of Pediatrics. 1994;124(1):105–111.
    1. Matthes T, Werner-Favre C, Tang H, Zhang X, Kindler V, Zubler RH. Cytokine mRNA expression during an in vitro response of human B lymphocytes: kinetics of B cell tumor necrosis factor α, interleukin (IL)6, IL-10, and transforming growth factor β1 mRNAs. Journal of Experimental Medicine. 1993;178(2):521–528.
    1. Martin H, Olander B, Norman M. Reactive hyperemia and interleukin 6, interleukin 8, and tumor necrosis factor-α in the diagnosis of early-onset neonatal sepsis. Pediatrics. 2001;108(4):1–6.
    1. Philip AG, Hewitt JR. Early diagnosis of neonatal sepsis. Pediatrics. 1980;65(5):1036–1041.
    1. Akenzua GI, Hui YT, Milner R, Zipursky A. Neutrophil and band counts in the diagnosis of neonatal infections. Pediatrics. 1974;54(1):38–42.
    1. Xanthou M. Leukocyte blood picture in ill newborn babies. Archives of Disease in Childhood. 1972;47(255):741–743.
    1. Manucha V, Rusia U, Sikka M, Faridi MMA, Madan N. Utility of haematological parameters and C-reactive protein in the detection of neonatal sepsis. Journal of Paediatrics and Child Health. 2002;38(5):459–464.
    1. Fowlie PW, Schmidt B. Diagnostic tests for bacterial infection from birth to 90 days—a systematic review. Archives of Disease in Childhood: Fetal and Neonatal Edition. 1998;78(2):F92–F98.
    1. Da Silva O, Ohlsson A, Kenyon C. Accuracy of leukocyte indices and C-reactive protein for diagnosis of neonatal sepsis: a critical review. Pediatric Infectious Disease Journal. 1995;14(5):362–366.
    1. Saez-Llorens X, Lagrutta SF. The acute phase host reaction during bacterial infection and its clinical impact in children. Pediatric Infectious Disease Journal. 1993;12(1):83–87.
    1. de Bont ES, Martens A, van Raan J, et al. Tumor necrosis factor-α, interleukin-1β, and interleukin-6 plasma levels in neonatal sepsis. Pediatric Research. 1993;33(4, part 1):380–383.
    1. Ozdemir A, Oygur N, Gultekin M, Coskun M, Yegin O. Neonatal tumor necrosis factor, interleukin-1α, interleukin-1β, and interleukin-6 response to infection. American Journal of Perinatology. 1994;11(4):282–285.
    1. Ng PC, Cheng SH, Chui KM, et al. Diagnosis of late onset neonatal sepsis with cytokines, adhesion molecule, and C-reactive protein in preterm very low birthweight infants. Archives of Disease in Childhood: Fetal and Neonatal Edition. 1997;77(3):F221–F227.
    1. Santana Reyes C, García-Muñoz F, Reyes D, González G, Dominguez C, Domenech E. Role of cytokines (interleukin-1β, 6, 8, tumour necrosis factor-α, and soluble receptor of interleukin-2) and C-reactive protein in the diagnosis of neonatal sepsis. Acta Paediatrica. 2003;92(2):221–227.
    1. Miller LC, Isa S, LoPreste G, Schaller JG, Dinarello CA. Neonatal interleukin-1β-, interleukin-6, and tumor necrosis factor: cord blood levels and cellular production. Journal of Pediatrics. 1990;117(6):961–965.
    1. Edgar JDM, Wilson DC, McMillan SA, et al. Predictive value of soluble immunological mediators in neonatal infection. Clinical Science. 1994;87(2):165–171.
    1. Kantar M, Kültürsay N, Kütükçüler N, Akisü M, Çetingül N, Çaglayan S. Plasma concentrations of granulocyte-macrophage colony-stimulating factor and interleukin-6 in septic and healthy preterms. European Journal of Pediatrics. 2000;159(3):156–157.
    1. Magudumana O, Ballot DE, Cooper PA, et al. Serial interleukin 6 measurements in the early diagnosis of neonatal sepsis. Journal of Tropical Pediatrics. 2000;46(5):267–271.
    1. Lehrnbecher T, Schrod L, Kraus D, Roos T, Martius J, von Stockhausen H-B. Interleukin-6 and soluble interleukin-6 receptor in cord blood in the diagnosis of early onset sepsis in neonates. Acta Paediatrica. 1995;84(7):806–808.
    1. Harris MC, Costarino AT, Jr., Sullivan JS, et al. Cytokine elevations in critically ill infants with sepsis and necrotizing enterocolitis. Journal of Pediatrics. 1994;124(1):105–111.
    1. Franz AR, Steinbach G, Kron M, Pohlandt F. Reduction of unnecessary antibiotic therapy in newborn infants using interleukin-8 and C-reactive protein as markers of bacterial infections. Pediatrics. 1999;104(3):447–453.
    1. Franz AR, Steinbach G, Kron M, Pohlandt F. Interleukin-8: a valuable tool to restrict antibiotic therapy in newborn infants. Acta Paediatrica. 2001;90(9):1025–1032.
    1. Kocabaş E, Sarikçioǧlu A, Aksaray N, Seydaoǧlu G, Seyhun Y, Yaman A. Role of procalcitonin, C-reactive protein, interleukin-6, interleukin-8 and tumor necrosis factor-α in the diagnosis of neonatal sepsis. Turkish Journal of Pediatrics. 2007;49(1):7–20.
    1. Messer J, Eyer D, Donato L, Gallati H, Martis J, Simeoni U. Evaluation of interleukin-6 and soluble receptors of tumor necrosis factor for early diagnosis of neonatal infection. Journal of Pediatrics. 1996;129(4):574–580.
    1. Oberhoffer M, Vogelsang H, Rußwurm S, Hartung T, Reinhart K. Outcome prediction by traditional and new markers of inflammation in patients with sepsis. Clinical Chemistry and Laboratory Medicine. 1999;37(3):363–368.

Source: PubMed

Szponzorok és közreműködők

Egészségi állapot

Kábítószer-beavatkozások

3
Iratkozz fel