Urinary bisphenol A concentrations and cytochrome P450 19 A1 (Cyp19) gene expression in ovarian granulosa cells: an in vivo human study

Abstract

Background: Exposure to bisphenol A (BPA), a chemical widely used in consumer products, has been associated with in vitro Cyp19 gene expression.

Objective: To evaluate an in vivo human model of Cyp19 gene expression in granulosa cells.

Study design: A subset of an ongoing prospective cohort study of women undergoing in vitro fertilization (IVF) at Massachusetts General Hospital.

Methods: Mixed effect models were used to evaluate the association of urinary BPA concentrations with granulosa cell Cyp19 mRNA expression.

Results: In 61 women undergoing 76 IVF cycles, adjusted changes in mean Cyp19 expression (β estimate (95% CI)) for quartiles 2, 3 and 4 as compared to the lowest quartile were: -0.97 (-2.22, 0.28); -0.97 (-2.18, 0.24) and -0.38 (-1.58, 0.82).

Conclusions: An in vivo model for evaluation of Cyp19 gene expression was developed for use in epidemiologic studies. In this pilot study, we found no statistically significant linear association between urinary BPA concentrations and Cyp19 expression.

Keywords: Bisphenol A; Cyp19; Fertility; Follicular fluid; Gene expression; Human; Ovary, granulosa cells; P450 aromatase.

Copyright © 2013 Elsevier Inc. All rights reserved.

Figures

Figure I. Quartiles of urinary BPA and Log CYP 19 gene expression

▲ Unadjusted ○ Adjusted for BMI and Batch/shipment (year 2012 versus year 2011) Q1, lowest quartile (≤1.32 μg/L); Q2, 2nd quartile (1.33-1.97 μg/L); Q3, 3rd quartile Q3 (1.98-3.26 μg/L); Q4, 4th quartile Q4 (≥3.27 μg/L)