Differential expression of microRNA expression in tamoxifen-sensitive MCF-7 versus tamoxifen-resistant LY2 human breast cancer cells
Tissa T Manavalan, Yun Teng, Savitri N Appana, Susmita Datta, Theodore S Kalbfleisch, Yong Li, Carolyn M Klinge, Tissa T Manavalan, Yun Teng, Savitri N Appana, Susmita Datta, Theodore S Kalbfleisch, Yong Li, Carolyn M Klinge
Abstract
Microarrays identified miRNAs differentially expressed and 4-hydroxytamoxifen (4-OHT) regulated in MCF-7 endocrine-sensitive versus resistant LY2 human breast cancer cells. 97 miRNAs were differentially expressed in MCF-7 versus LY2 cells. Opposite expression of miRs-10a, 21, 22, 29a, 93, 125b, 181, 200a, 200b, 200c, 205, and 222 was confirmed. Bioinformatic analyses to impute the biological significance of these miRNAs identified 36 predicted gene targets from those regulated by 4-OHT in MCF-7 cells. Agreement in the direction of anticipated regulation was detected for 12 putative targets. These miRNAs with opposite expression between the two cell lines may be involved in endocrine resistance.
Conflict of interest statement
Conflict of interest
The authors declare that they have no conflict of interest.
Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
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Source: PubMed