BliI, a restriction endonuclease from Bacillus licheniformis
P L Manachini, C Parini, M G Fortina, L Benazzi, P L Manachini, C Parini, M G Fortina, L Benazzi
Abstract
From Bacillus licheniformis a site-specific restriction endonuclease, named BliI, has been purified and characterized. BliI was able to digest lambda DNA at pH 9.1 over a wide temperature range (25-65 degrees C). Digestion of lambda and psi X174 DNAs with BliI produced banding patterns identical to those seen with HaeIII. Therefore, BliI and HaeIII endonculeases are isoschizomers.
Source: PubMed