Skeletal Muscle myomiR Are Differentially Expressed by Endurance Exercise Mode and Combined Essential Amino Acid and Carbohydrate Supplementation

Lee M Margolis, Holly L McClung, Nancy E Murphy, Christopher T Carrigan, Stefan M Pasiakos, Lee M Margolis, Holly L McClung, Nancy E Murphy, Christopher T Carrigan, Stefan M Pasiakos

Abstract

Skeletal muscle microRNAs (myomiR) expression is modulated by exercise, however, the influence of endurance exercise mode, combined with essential amino acid and carbohydrate (EAA+CHO) supplementation are not well defined. This study determined the effects of weighted versus non-weighted endurance exercise, with or without EAA+CHO ingestion on myomiR expression and their association with muscle protein synthesis (MPS). Twenty five adults performed 90 min of metabolically-matched (2.2 VO2 L·m-1) load carriage (LC; performed on a treadmill wearing a vest equal to 30% of individual body mass) or cycle ergometry (CE) exercise, during which EAA+CHO (10 g EAA and 46 g CHO) or non-nutritive control (CON) drinks were consumed. Expression of myomiR (RT-qPCR) were determined at rest (PRE), immediately post-exercise (POST), and 3 h into recovery (REC). Muscle protein synthesis (2H5-phenylalanine) was measured during exercise and recovery. Relative to PRE, POST, and REC expression of miR-1-3p, miR-206, miR-208a-5, and miR-499 was lower (P < 0.05) for LC compared to CE, regardless of dietary treatment. Independent of exercise mode, miR-1-3p and miR-208a-5p expression were lower (P < 0.05) after ingesting EAA+CHO compared to CON. Expression of miR-206 was highest for CE-CON than any other treatment (exercise-by-drink, P < 0.05). Common targets of differing myomiR were identified as markers within mTORC1 signaling, and miR-206 and miR-499 were inversely associated with MPS rates immediately post-exercise. These findings suggest the alterations in myomiR expression between exercise mode and EAA+CHO intake may in part be due to differing MPS modulation immediately post-exercise.

Keywords: Akt; cycle ergometry; load carriage; myomiR; rpS6.

Figures

Figure 1
Figure 1
Data are mean ± SD. Expression of miR-1-3p (A), miR-206 (B), miR-208a-5p (C), and miR-499 (D) at Baseline (■), Post-exercise () and Recovery (□). *Load carriage different than Cycle Ergometry; P < 0.05. †EAA+CHO (essential amino acid + carbohydrate) different than CON (non-nutritive control); P < 0.05. ‡Exercise-by-drink interaction; Cycle Ergometry-CON different than Cycle Ergometry-EAA+CHO; P < 0.05.
Figure 2
Figure 2
Schematic of myomiR interaction with Akt-mTORC1 signaling pathway.
Figure 3
Figure 3
Data are mean ± SD. Phosphorylation status of IRS1Ser302(A), AktSer473(B), p70S6KThr389(C), and rpS6Ser235/236(D) at Baseline (■) and Post-exercise ().+Post-exercise different than Baseline; P < 0.05.
Figure 4
Figure 4
Correlation of miR-206 (A) and miR-499 (B) to muscle protein synthesis post-exercise.

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