Development of a rapid test kit for SARS-CoV-2: an example of product design
Zhanfeng Cui, Hong Chang, Hui Wang, Boon Lim, Chia-Chen Hsu, Yejiong Yu, Huidong Jia, Yun Wang, Yida Zeng, Mengmeng Ji, Weizhi Liu, Catriona Inverarity, Wei E Huang, Zhanfeng Cui, Hong Chang, Hui Wang, Boon Lim, Chia-Chen Hsu, Yejiong Yu, Huidong Jia, Yun Wang, Yida Zeng, Mengmeng Ji, Weizhi Liu, Catriona Inverarity, Wei E Huang
Abstract
We present an example of applying 'need-driven' product design principle to the development of a rapid test kit to detect SARS-COV-2 (COVID-19). The tests are intended for use in the field and, longer term, for home use. They detect whether a subject is currently infected with the virus and is infectious. The urgent need for large numbers of tests in field setting imposes constraints such as short test time and lack of access to specialist equipment, laboratories and skilled technicians to perform the test and interpret results. To meet these needs, an antigen test based on RT-LAMP with colorimetric readout was chosen. Direct use of swab sample with no RNA extraction was explored. After extensive experimental study (reported elsewhere), a rapid test kit has been fabricated to satisfy all design criteria.
Keywords: Antigen detection; COVID-19; Product design; RT-LAMP; Rapid test; SAR-Cov-2.
Conflict of interest statement
Conflict of interestThe authors declare that there is no conflict of interest.
© The Author(s) 2020.
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References
- Cussler EL, Moggridge GD. Chemical product design. 2. Cambridge: Cambridge University Press; 2011.
- Huang WE, Lim B, Hsu CC, Xiong D, Wu W, Yu Y, Jia H, Wang Y, Zeng Y, Ji M, Chang H. RT-LAMP for rapid diagnosis of coronavirus SARS-CoV-2. Microb Biotechnol. 2020 doi: 10.1111/1751-7915.13586.
- Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000;28(12):63e. doi: 10.1093/nar/28.12.e63.
- Tomita N, Mori Y, Kanda H, Notomi T. Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nat Protocols. 2008;3:877–882. doi: 10.1038/nprot.2008.57.
Source: PubMed