Collagen-based silver nanoparticles for biological applications: synthesis and characterization

Vinicius S Cardoso, Patrick V Quelemes, Adriany Amorin, Fernando Lucas Primo, Graciely Gomides Gobo, Antonio C Tedesco, Ana C Mafud, Yvonne P Mascarenhas, José Raimundo Corrêa, Selma A S Kuckelhaus, Carla Eiras, José Roberto S A Leite, Durcilene Silva, José Ribeiro dos Santos Júnior, Vinicius S Cardoso, Patrick V Quelemes, Adriany Amorin, Fernando Lucas Primo, Graciely Gomides Gobo, Antonio C Tedesco, Ana C Mafud, Yvonne P Mascarenhas, José Raimundo Corrêa, Selma A S Kuckelhaus, Carla Eiras, José Roberto S A Leite, Durcilene Silva, José Ribeiro dos Santos Júnior

Abstract

Background: Type I collagen is an abundant natural polymer with several applications in medicine as matrix to regenerate tissues. Silver nanoparticles is an important nanotechnology material with many utilities in some areas such as medicine, biology and chemistry. The present study focused on the synthesis of silver nanoparticles (AgNPs) stabilized with type I collagen (AgNPcol) to build a nanomaterial with biological utility. Three formulations of AgNPcol were physicochemical characterized, antibacterial activity in vitro and cell viability assays were analyzed. AgNPcol was characterized by means of the following: ultraviolet-visible spectroscopy, dynamic light scattering analysis, Fourier transform infrared spectroscopy, atomic absorption analysis, transmission electron microscopy and of X-ray diffraction analysis.

Results: All AgNPcol showed spherical and positive zeta potential. The AgNPcol at a molar ratio of 1:6 showed better characteristics, smaller hydrodynamic diameter (64.34 ± 16.05) and polydispersity index (0.40 ± 0.05), and higher absorbance and silver reduction efficiency (0.645 mM), when compared with the particles prepared in other mixing ratios. Furthermore, these particles showed antimicrobial activity against both Staphylococcus aureus and Escherichia coli and no toxicity to the cells at the examined concentrations.

Conclusions: The resulted particles exhibited favorable characteristics, including the spherical shape, diameter between 64.34 nm and 81.76 nm, positive zeta potential, antibacterial activity, and non-toxicity to the tested cells (OSCC).

Figures

Figure 1
Figure 1
AgNPcol characterization. (A) Absorbance spectra of AgNPcols at three different NaBH4 to AgNO3 molar ratios; (B) FTIR spectra of collagen; (C) XDR patterns of AgNPcol (1:6 molar ratio); (D) FTIR spectra of AgNPcol (1:6 molar ratio).
Figure 2
Figure 2
Images of Silver nanoparticle stabilized with collagen. TEM images of AgNPcol at AgNO3/NaBH4 molar ratio of (A) 1:1, (B) 1:6, and (C) 1:15 molar ratio. Histograms showing the particle size distribution of AgNPcol at molar ratio of (D) 1:1 (28.11 ± 10 nm), (E) 1:6 (15.17 ± 2.71 nm), and (F) 1:15 (28.17 ± 9.45 nm) (scale bar = 0.1 μm).
Figure 3
Figure 3
Cell viability. Results of cell viability test of AgNPcol and control solutions (Col and AgNO3). All data were expressed as mean ± SEM values of three independent experiments. A value of *p < 0.05 was considered statistically significance.

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