Cigarette smoke dissociates inflammation and lung remodeling in OVA-sensitized and challenged mice
Deborah C Hizume, Alessandra C Toledo, Henrique T Moriya, Beatriz M Saraiva-Romanholo, Francine M Almeida, Fernanda M Arantes-Costa, Rodolfo P Vieira, Marisa Dolhnikoff, David Itiro Kasahara, Milton A Martins, Deborah C Hizume, Alessandra C Toledo, Henrique T Moriya, Beatriz M Saraiva-Romanholo, Francine M Almeida, Fernanda M Arantes-Costa, Rodolfo P Vieira, Marisa Dolhnikoff, David Itiro Kasahara, Milton A Martins
Abstract
We evaluated the effects of cigarette smoke (CS) on lung inflammation and remodeling in a model of ovalbumin (OVA)-sensitized and OVA-challenged mice. Male BALB/c mice were divided into 4 groups: non-sensitized and air-exposed (control); non-sensitized and exposed to cigarette smoke (CS), sensitized and air-exposed (OVA) (50 μg+OVA 1% 3 times/week for 3 weeks) and sensitized and cigarette smoke exposed mice (OVA+CS). IgE levels were not affected by CS exposure. The increases in total bronchoalveolar fluid cells in the OVA group were attenuated by co-exposure to CS, as were the changes in IL-4, IL-5, and eotaxin levels as well as tissue elastance (p<0.05). In contrast, only the OVA+CS group showed a significant increase in the protein expression of IFN-γ, VEGF, GM-CSF and collagen fiber content (p<0.05). In our study, exposure to cigarette smoke in OVA-challenged mice resulted in an attenuation of pulmonary inflammation but led to an increase in pulmonary remodeling and resulted in the dissociation of airway inflammation from lung remodeling.
Copyright © 2012 Elsevier B.V. All rights reserved.
Source: PubMed