Mesenchymal stem cells and endothelial progenitor cells decrease renal injury in experimental swine renal artery stenosis through different mechanisms
Xiang-Yang Zhu, Victor Urbieta-Caceres, James D Krier, Stephen C Textor, Amir Lerman, Lilach O Lerman, Xiang-Yang Zhu, Victor Urbieta-Caceres, James D Krier, Stephen C Textor, Amir Lerman, Lilach O Lerman
Abstract
Endothelial progenitor cells (EPC) and mesenchymal stem cells (MSC) augment tissue repair but possess slightly different properties. How the cellular phenotype affects the efficacy of this approach in renovascular disease is incompletely understood. This study tested the hypothesis that EPC and MSC protect the poststenotic kidney by blunting different disease pathways. Peripheral blood EPC and adipose-derived MSC were expanded and characterized by cell surface markers (e.g., CD34/kinase insert domain receptor, or CD44/CD90). Single-kidney hemodynamics and function were assessed in pigs after 10 weeks of renal artery stenosis (RAS) treated 4 weeks earlier with an intrarenal infusion of vehicle (n = 7), EPC (RAS+EPC) or MSC (RAS+MSC) (both 10 × 10(6), n = 6), and normal controls (n = 7). Kidney disease mechanisms were evaluated ex vivo. The ability of EPC and MSC to attenuate endoplasmic reticulum (ER) stress was also studied in isolated ER and in tubular cells cocultured with EPC and MSC. Glomerular filtration rate in RAS was lower than controls, increased in RAS+EPC, and further improved in RAS+MSC, although both improved renal blood flow similarly. EPC prominently enhanced renal growth factor expression and decreased oxidative stress, while MSC more significantly attenuated renal inflammation, ER stress, and apoptosis. Furthermore, MSC induced a greater decrease in caspase-3 and CHOP expression in cultured tubular cells through mechanisms involving cell contact. EPC and MSC achieve a comparable decrease of kidney injury in RAS by different mechanisms, although MSC elicited slightly superior improvement of renal function. These results support development of cell-based approaches for management of renovascular disease and suggest cell selection based on the underlying pathophysiology of kidney injury.
Copyright © 2012 AlphaMed Press.
Figures
![Figure 1](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3528811/bin/nihms420802f1.jpg)
Figure 2
Top: Representative images of CM-DiI…
Figure 2
Top: Representative images of CM-DiI labeled (red) endothelial progenitor cells (EPC) or mesenchymal…
Figure 3
Representative micro-CT images of pig…
Figure 3
Representative micro-CT images of pig kidney samples from normal, renal artery stenosis (RAS),…
Figure 4
Western blotting images and quantification…
Figure 4
Western blotting images and quantification of renal angiogenic (A), oxidative and inflammatory (B),…
Figure 5
Representative images of trichrome (blue,…
Figure 5
Representative images of trichrome (blue, top 20x) and TUNEL (middle, green, arrow) staining…
Figure 6
Top: Western blotting images and…
Figure 6
Top: Western blotting images and quantification of the expression of CHOP and caspase-3…
- Adipose tissue-derived mesenchymal stem cells improve revascularization outcomes to restore renal function in swine atherosclerotic renal artery stenosis.Eirin A, Zhu XY, Krier JD, Tang H, Jordan KL, Grande JP, Lerman A, Textor SC, Lerman LO. Eirin A, et al. Stem Cells. 2012 May;30(5):1030-41. doi: 10.1002/stem.1047. Stem Cells. 2012. PMID: 22290832 Free PMC article.
- Mesenchymal Stem/Stromal Cells and their Extracellular Vesicle Progeny Decrease Injury in Poststenotic Swine Kidney Through Different Mechanisms.Zhao Y, Zhu X, Zhang L, Ferguson CM, Song T, Jiang K, Conley SM, Krier JD, Tang H, Saadiq I, Jordan KL, Lerman A, Lerman LO. Zhao Y, et al. Stem Cells Dev. 2020 Sep 15;29(18):1190-1200. doi: 10.1089/scd.2020.0030. Epub 2020 Aug 3. Stem Cells Dev. 2020. PMID: 32657229 Free PMC article.
- Adjunctive mesenchymal stem/stromal cells augment microvascular function in poststenotic kidneys treated with low-energy shockwave therapy.Chen XJ, Zhang X, Jiang K, Krier JD, Zhu X, Conley S, Lerman A, Lerman LO. Chen XJ, et al. J Cell Physiol. 2020 Dec;235(12):9806-9818. doi: 10.1002/jcp.29794. Epub 2020 May 19. J Cell Physiol. 2020. PMID: 32430932 Free PMC article.
- Addition of endothelial progenitor cells to renal revascularization restores medullary tubular oxygen consumption in swine renal artery stenosis.Ebrahimi B, Li Z, Eirin A, Zhu XY, Textor SC, Lerman LO. Ebrahimi B, et al. Am J Physiol Renal Physiol. 2012 Jun 1;302(11):F1478-85. doi: 10.1152/ajprenal.00563.2011. Epub 2012 Mar 14. Am J Physiol Renal Physiol. 2012. PMID: 22419692 Free PMC article.
- Stem cells in kidney diseases.Soler MJ, José Tomas OP. Soler MJ, et al. J Stem Cells. 2012;7(4):245-59. J Stem Cells. 2012. PMID: 24196799 Review.
- Immunological priming of mesenchymal stromal/stem cells and their extracellular vesicles augments their therapeutic benefits in experimental graft-versus-host disease via engagement of PD-1 ligands.Hackel A, Vollmer S, Bruderek K, Lang S, Brandau S. Hackel A, et al. Front Immunol. 2023 Feb 16;14:1078551. doi: 10.3389/fimmu.2023.1078551. eCollection 2023. Front Immunol. 2023. PMID: 36875112 Free PMC article.
- Autologous Extracellular Vesicles Attenuate Cardiac Injury in Experimental Atherosclerotic Renovascular Disease More Effectively Than Their Parent Mesenchymal Stem/Stromal Cells.Hong S, Zhu XY, Jiang Y, Zhang L, Tang H, Jordan KL, Saadiq IM, Huang W, Lerman A, Eirin A, Lerman LO. Hong S, et al. Stem Cell Rev Rep. 2023 Apr;19(3):700-712. doi: 10.1007/s12015-022-10473-2. Epub 2022 Nov 7. Stem Cell Rev Rep. 2023. PMID: 36344721
- Effects of mesenchymal stem cells in renovascular disease of preclinical and clinical studies: a systematic review and meta-analysis.Wang HS, Yi MY, Wu X, Liu Q, Deng YH, Wu T, Wang L, Kang YX, Luo XQ, Yan P, Wang M, Duan SB. Wang HS, et al. Sci Rep. 2022 Oct 27;12(1):18080. doi: 10.1038/s41598-022-23059-2. Sci Rep. 2022. PMID: 36302933 Free PMC article.
- Mesenchymal stem cells: A new therapeutic tool for chronic kidney disease.Wang J, Lin Y, Chen X, Liu Y, Zhou T. Wang J, et al. Front Cell Dev Biol. 2022 Oct 4;10:910592. doi: 10.3389/fcell.2022.910592. eCollection 2022. Front Cell Dev Biol. 2022. PMID: 36268508 Free PMC article. Review.
- Effect of Hypoxia Preconditioning on the Regenerative Capacity of Adipose Tissue Derived Mesenchymal Stem Cells in a Model of Renal Artery Stenosis.Farooqui N, Mohan A, Isik B, Goksu BB, Thaler R, Zhu XY, Krier JD, Saadiq IM, Ferguson CM, Jordan KL, Tang H, Textor SC, Hickson TJ, van Wijnen AJ, Eirin A, Lerman LO, Herrmann SM. Farooqui N, et al. Stem Cells. 2023 Jan 30;41(1):50-63. doi: 10.1093/stmcls/sxac073. Stem Cells. 2023. PMID: 36250949
- Research Support, N.I.H., Extramural
- Acute Kidney Injury / therapy*
- Animals
- Antigens, CD34
- Apoptosis
- Caspase 3 / metabolism
- Cell- and Tissue-Based Therapy
- Cells, Cultured
- Endoplasmic Reticulum Stress
- Endothelial Cells / metabolism*
- Glomerular Filtration Rate
- Hyaluronan Receptors
- Kidney / blood supply
- Kidney / metabolism
- Kidney / pathology
- Mesenchymal Stem Cells / metabolism*
- Oxidative Stress
- Renal Artery Obstruction / metabolism*
- Renal Artery Obstruction / pathology
- Renal Circulation
- Swine
- Thy-1 Antigens
- Antigens, CD34
- Hyaluronan Receptors
- Thy-1 Antigens
- Caspase 3
- Full Text Sources
- Medical
- Research Materials
- Miscellaneous
NCBI Literature Resources
The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Unauthorized use of these marks is strictly prohibited.
National Library of Medicine
8600 Rockville Pike
Bethesda, MD 20894
![Figure 2](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3528811/bin/nihms420802f2.jpg)
Figure 3
Representative micro-CT images of pig…
Figure 3
Representative micro-CT images of pig kidney samples from normal, renal artery stenosis (RAS),…
Figure 4
Western blotting images and quantification…
Figure 4
Western blotting images and quantification of renal angiogenic (A), oxidative and inflammatory (B),…
Figure 5
Representative images of trichrome (blue,…
Figure 5
Representative images of trichrome (blue, top 20x) and TUNEL (middle, green, arrow) staining…
Figure 6
Top: Western blotting images and…
Figure 6
Top: Western blotting images and quantification of the expression of CHOP and caspase-3…
- Adipose tissue-derived mesenchymal stem cells improve revascularization outcomes to restore renal function in swine atherosclerotic renal artery stenosis.Eirin A, Zhu XY, Krier JD, Tang H, Jordan KL, Grande JP, Lerman A, Textor SC, Lerman LO. Eirin A, et al. Stem Cells. 2012 May;30(5):1030-41. doi: 10.1002/stem.1047. Stem Cells. 2012. PMID: 22290832 Free PMC article.
- Mesenchymal Stem/Stromal Cells and their Extracellular Vesicle Progeny Decrease Injury in Poststenotic Swine Kidney Through Different Mechanisms.Zhao Y, Zhu X, Zhang L, Ferguson CM, Song T, Jiang K, Conley SM, Krier JD, Tang H, Saadiq I, Jordan KL, Lerman A, Lerman LO. Zhao Y, et al. Stem Cells Dev. 2020 Sep 15;29(18):1190-1200. doi: 10.1089/scd.2020.0030. Epub 2020 Aug 3. Stem Cells Dev. 2020. PMID: 32657229 Free PMC article.
- Adjunctive mesenchymal stem/stromal cells augment microvascular function in poststenotic kidneys treated with low-energy shockwave therapy.Chen XJ, Zhang X, Jiang K, Krier JD, Zhu X, Conley S, Lerman A, Lerman LO. Chen XJ, et al. J Cell Physiol. 2020 Dec;235(12):9806-9818. doi: 10.1002/jcp.29794. Epub 2020 May 19. J Cell Physiol. 2020. PMID: 32430932 Free PMC article.
- Addition of endothelial progenitor cells to renal revascularization restores medullary tubular oxygen consumption in swine renal artery stenosis.Ebrahimi B, Li Z, Eirin A, Zhu XY, Textor SC, Lerman LO. Ebrahimi B, et al. Am J Physiol Renal Physiol. 2012 Jun 1;302(11):F1478-85. doi: 10.1152/ajprenal.00563.2011. Epub 2012 Mar 14. Am J Physiol Renal Physiol. 2012. PMID: 22419692 Free PMC article.
- Stem cells in kidney diseases.Soler MJ, José Tomas OP. Soler MJ, et al. J Stem Cells. 2012;7(4):245-59. J Stem Cells. 2012. PMID: 24196799 Review.
- Immunological priming of mesenchymal stromal/stem cells and their extracellular vesicles augments their therapeutic benefits in experimental graft-versus-host disease via engagement of PD-1 ligands.Hackel A, Vollmer S, Bruderek K, Lang S, Brandau S. Hackel A, et al. Front Immunol. 2023 Feb 16;14:1078551. doi: 10.3389/fimmu.2023.1078551. eCollection 2023. Front Immunol. 2023. PMID: 36875112 Free PMC article.
- Autologous Extracellular Vesicles Attenuate Cardiac Injury in Experimental Atherosclerotic Renovascular Disease More Effectively Than Their Parent Mesenchymal Stem/Stromal Cells.Hong S, Zhu XY, Jiang Y, Zhang L, Tang H, Jordan KL, Saadiq IM, Huang W, Lerman A, Eirin A, Lerman LO. Hong S, et al. Stem Cell Rev Rep. 2023 Apr;19(3):700-712. doi: 10.1007/s12015-022-10473-2. Epub 2022 Nov 7. Stem Cell Rev Rep. 2023. PMID: 36344721
- Effects of mesenchymal stem cells in renovascular disease of preclinical and clinical studies: a systematic review and meta-analysis.Wang HS, Yi MY, Wu X, Liu Q, Deng YH, Wu T, Wang L, Kang YX, Luo XQ, Yan P, Wang M, Duan SB. Wang HS, et al. Sci Rep. 2022 Oct 27;12(1):18080. doi: 10.1038/s41598-022-23059-2. Sci Rep. 2022. PMID: 36302933 Free PMC article.
- Mesenchymal stem cells: A new therapeutic tool for chronic kidney disease.Wang J, Lin Y, Chen X, Liu Y, Zhou T. Wang J, et al. Front Cell Dev Biol. 2022 Oct 4;10:910592. doi: 10.3389/fcell.2022.910592. eCollection 2022. Front Cell Dev Biol. 2022. PMID: 36268508 Free PMC article. Review.
- Effect of Hypoxia Preconditioning on the Regenerative Capacity of Adipose Tissue Derived Mesenchymal Stem Cells in a Model of Renal Artery Stenosis.Farooqui N, Mohan A, Isik B, Goksu BB, Thaler R, Zhu XY, Krier JD, Saadiq IM, Ferguson CM, Jordan KL, Tang H, Textor SC, Hickson TJ, van Wijnen AJ, Eirin A, Lerman LO, Herrmann SM. Farooqui N, et al. Stem Cells. 2023 Jan 30;41(1):50-63. doi: 10.1093/stmcls/sxac073. Stem Cells. 2023. PMID: 36250949
- Research Support, N.I.H., Extramural
- Acute Kidney Injury / therapy*
- Animals
- Antigens, CD34
- Apoptosis
- Caspase 3 / metabolism
- Cell- and Tissue-Based Therapy
- Cells, Cultured
- Endoplasmic Reticulum Stress
- Endothelial Cells / metabolism*
- Glomerular Filtration Rate
- Hyaluronan Receptors
- Kidney / blood supply
- Kidney / metabolism
- Kidney / pathology
- Mesenchymal Stem Cells / metabolism*
- Oxidative Stress
- Renal Artery Obstruction / metabolism*
- Renal Artery Obstruction / pathology
- Renal Circulation
- Swine
- Thy-1 Antigens
- Antigens, CD34
- Hyaluronan Receptors
- Thy-1 Antigens
- Caspase 3
- Full Text Sources
- Medical
- Research Materials
- Miscellaneous
NCBI Literature Resources
The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Unauthorized use of these marks is strictly prohibited.
National Library of Medicine
8600 Rockville Pike
Bethesda, MD 20894
![Figure 3](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3528811/bin/nihms420802f3.jpg)
Figure 4
Western blotting images and quantification…
Figure 4
Western blotting images and quantification of renal angiogenic (A), oxidative and inflammatory (B),…
Figure 5
Representative images of trichrome (blue,…
Figure 5
Representative images of trichrome (blue, top 20x) and TUNEL (middle, green, arrow) staining…
Figure 6
Top: Western blotting images and…
Figure 6
Top: Western blotting images and quantification of the expression of CHOP and caspase-3…
- Adipose tissue-derived mesenchymal stem cells improve revascularization outcomes to restore renal function in swine atherosclerotic renal artery stenosis.Eirin A, Zhu XY, Krier JD, Tang H, Jordan KL, Grande JP, Lerman A, Textor SC, Lerman LO. Eirin A, et al. Stem Cells. 2012 May;30(5):1030-41. doi: 10.1002/stem.1047. Stem Cells. 2012. PMID: 22290832 Free PMC article.
- Mesenchymal Stem/Stromal Cells and their Extracellular Vesicle Progeny Decrease Injury in Poststenotic Swine Kidney Through Different Mechanisms.Zhao Y, Zhu X, Zhang L, Ferguson CM, Song T, Jiang K, Conley SM, Krier JD, Tang H, Saadiq I, Jordan KL, Lerman A, Lerman LO. Zhao Y, et al. Stem Cells Dev. 2020 Sep 15;29(18):1190-1200. doi: 10.1089/scd.2020.0030. Epub 2020 Aug 3. Stem Cells Dev. 2020. PMID: 32657229 Free PMC article.
- Adjunctive mesenchymal stem/stromal cells augment microvascular function in poststenotic kidneys treated with low-energy shockwave therapy.Chen XJ, Zhang X, Jiang K, Krier JD, Zhu X, Conley S, Lerman A, Lerman LO. Chen XJ, et al. J Cell Physiol. 2020 Dec;235(12):9806-9818. doi: 10.1002/jcp.29794. Epub 2020 May 19. J Cell Physiol. 2020. PMID: 32430932 Free PMC article.
- Addition of endothelial progenitor cells to renal revascularization restores medullary tubular oxygen consumption in swine renal artery stenosis.Ebrahimi B, Li Z, Eirin A, Zhu XY, Textor SC, Lerman LO. Ebrahimi B, et al. Am J Physiol Renal Physiol. 2012 Jun 1;302(11):F1478-85. doi: 10.1152/ajprenal.00563.2011. Epub 2012 Mar 14. Am J Physiol Renal Physiol. 2012. PMID: 22419692 Free PMC article.
- Stem cells in kidney diseases.Soler MJ, José Tomas OP. Soler MJ, et al. J Stem Cells. 2012;7(4):245-59. J Stem Cells. 2012. PMID: 24196799 Review.
- Immunological priming of mesenchymal stromal/stem cells and their extracellular vesicles augments their therapeutic benefits in experimental graft-versus-host disease via engagement of PD-1 ligands.Hackel A, Vollmer S, Bruderek K, Lang S, Brandau S. Hackel A, et al. Front Immunol. 2023 Feb 16;14:1078551. doi: 10.3389/fimmu.2023.1078551. eCollection 2023. Front Immunol. 2023. PMID: 36875112 Free PMC article.
- Autologous Extracellular Vesicles Attenuate Cardiac Injury in Experimental Atherosclerotic Renovascular Disease More Effectively Than Their Parent Mesenchymal Stem/Stromal Cells.Hong S, Zhu XY, Jiang Y, Zhang L, Tang H, Jordan KL, Saadiq IM, Huang W, Lerman A, Eirin A, Lerman LO. Hong S, et al. Stem Cell Rev Rep. 2023 Apr;19(3):700-712. doi: 10.1007/s12015-022-10473-2. Epub 2022 Nov 7. Stem Cell Rev Rep. 2023. PMID: 36344721
- Effects of mesenchymal stem cells in renovascular disease of preclinical and clinical studies: a systematic review and meta-analysis.Wang HS, Yi MY, Wu X, Liu Q, Deng YH, Wu T, Wang L, Kang YX, Luo XQ, Yan P, Wang M, Duan SB. Wang HS, et al. Sci Rep. 2022 Oct 27;12(1):18080. doi: 10.1038/s41598-022-23059-2. Sci Rep. 2022. PMID: 36302933 Free PMC article.
- Mesenchymal stem cells: A new therapeutic tool for chronic kidney disease.Wang J, Lin Y, Chen X, Liu Y, Zhou T. Wang J, et al. Front Cell Dev Biol. 2022 Oct 4;10:910592. doi: 10.3389/fcell.2022.910592. eCollection 2022. Front Cell Dev Biol. 2022. PMID: 36268508 Free PMC article. Review.
- Effect of Hypoxia Preconditioning on the Regenerative Capacity of Adipose Tissue Derived Mesenchymal Stem Cells in a Model of Renal Artery Stenosis.Farooqui N, Mohan A, Isik B, Goksu BB, Thaler R, Zhu XY, Krier JD, Saadiq IM, Ferguson CM, Jordan KL, Tang H, Textor SC, Hickson TJ, van Wijnen AJ, Eirin A, Lerman LO, Herrmann SM. Farooqui N, et al. Stem Cells. 2023 Jan 30;41(1):50-63. doi: 10.1093/stmcls/sxac073. Stem Cells. 2023. PMID: 36250949
- Research Support, N.I.H., Extramural
- Acute Kidney Injury / therapy*
- Animals
- Antigens, CD34
- Apoptosis
- Caspase 3 / metabolism
- Cell- and Tissue-Based Therapy
- Cells, Cultured
- Endoplasmic Reticulum Stress
- Endothelial Cells / metabolism*
- Glomerular Filtration Rate
- Hyaluronan Receptors
- Kidney / blood supply
- Kidney / metabolism
- Kidney / pathology
- Mesenchymal Stem Cells / metabolism*
- Oxidative Stress
- Renal Artery Obstruction / metabolism*
- Renal Artery Obstruction / pathology
- Renal Circulation
- Swine
- Thy-1 Antigens
- Antigens, CD34
- Hyaluronan Receptors
- Thy-1 Antigens
- Caspase 3
- Full Text Sources
- Medical
- Research Materials
- Miscellaneous
NCBI Literature Resources
The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Unauthorized use of these marks is strictly prohibited.
National Library of Medicine
8600 Rockville Pike
Bethesda, MD 20894
![Figure 4](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3528811/bin/nihms420802f4.jpg)
Figure 5
Representative images of trichrome (blue,…
Figure 5
Representative images of trichrome (blue, top 20x) and TUNEL (middle, green, arrow) staining…
Figure 6
Top: Western blotting images and…
Figure 6
Top: Western blotting images and quantification of the expression of CHOP and caspase-3…
- Adipose tissue-derived mesenchymal stem cells improve revascularization outcomes to restore renal function in swine atherosclerotic renal artery stenosis.Eirin A, Zhu XY, Krier JD, Tang H, Jordan KL, Grande JP, Lerman A, Textor SC, Lerman LO. Eirin A, et al. Stem Cells. 2012 May;30(5):1030-41. doi: 10.1002/stem.1047. Stem Cells. 2012. PMID: 22290832 Free PMC article.
- Mesenchymal Stem/Stromal Cells and their Extracellular Vesicle Progeny Decrease Injury in Poststenotic Swine Kidney Through Different Mechanisms.Zhao Y, Zhu X, Zhang L, Ferguson CM, Song T, Jiang K, Conley SM, Krier JD, Tang H, Saadiq I, Jordan KL, Lerman A, Lerman LO. Zhao Y, et al. Stem Cells Dev. 2020 Sep 15;29(18):1190-1200. doi: 10.1089/scd.2020.0030. Epub 2020 Aug 3. Stem Cells Dev. 2020. PMID: 32657229 Free PMC article.
- Adjunctive mesenchymal stem/stromal cells augment microvascular function in poststenotic kidneys treated with low-energy shockwave therapy.Chen XJ, Zhang X, Jiang K, Krier JD, Zhu X, Conley S, Lerman A, Lerman LO. Chen XJ, et al. J Cell Physiol. 2020 Dec;235(12):9806-9818. doi: 10.1002/jcp.29794. Epub 2020 May 19. J Cell Physiol. 2020. PMID: 32430932 Free PMC article.
- Addition of endothelial progenitor cells to renal revascularization restores medullary tubular oxygen consumption in swine renal artery stenosis.Ebrahimi B, Li Z, Eirin A, Zhu XY, Textor SC, Lerman LO. Ebrahimi B, et al. Am J Physiol Renal Physiol. 2012 Jun 1;302(11):F1478-85. doi: 10.1152/ajprenal.00563.2011. Epub 2012 Mar 14. Am J Physiol Renal Physiol. 2012. PMID: 22419692 Free PMC article.
- Stem cells in kidney diseases.Soler MJ, José Tomas OP. Soler MJ, et al. J Stem Cells. 2012;7(4):245-59. J Stem Cells. 2012. PMID: 24196799 Review.
- Immunological priming of mesenchymal stromal/stem cells and their extracellular vesicles augments their therapeutic benefits in experimental graft-versus-host disease via engagement of PD-1 ligands.Hackel A, Vollmer S, Bruderek K, Lang S, Brandau S. Hackel A, et al. Front Immunol. 2023 Feb 16;14:1078551. doi: 10.3389/fimmu.2023.1078551. eCollection 2023. Front Immunol. 2023. PMID: 36875112 Free PMC article.
- Autologous Extracellular Vesicles Attenuate Cardiac Injury in Experimental Atherosclerotic Renovascular Disease More Effectively Than Their Parent Mesenchymal Stem/Stromal Cells.Hong S, Zhu XY, Jiang Y, Zhang L, Tang H, Jordan KL, Saadiq IM, Huang W, Lerman A, Eirin A, Lerman LO. Hong S, et al. Stem Cell Rev Rep. 2023 Apr;19(3):700-712. doi: 10.1007/s12015-022-10473-2. Epub 2022 Nov 7. Stem Cell Rev Rep. 2023. PMID: 36344721
- Effects of mesenchymal stem cells in renovascular disease of preclinical and clinical studies: a systematic review and meta-analysis.Wang HS, Yi MY, Wu X, Liu Q, Deng YH, Wu T, Wang L, Kang YX, Luo XQ, Yan P, Wang M, Duan SB. Wang HS, et al. Sci Rep. 2022 Oct 27;12(1):18080. doi: 10.1038/s41598-022-23059-2. Sci Rep. 2022. PMID: 36302933 Free PMC article.
- Mesenchymal stem cells: A new therapeutic tool for chronic kidney disease.Wang J, Lin Y, Chen X, Liu Y, Zhou T. Wang J, et al. Front Cell Dev Biol. 2022 Oct 4;10:910592. doi: 10.3389/fcell.2022.910592. eCollection 2022. Front Cell Dev Biol. 2022. PMID: 36268508 Free PMC article. Review.
- Effect of Hypoxia Preconditioning on the Regenerative Capacity of Adipose Tissue Derived Mesenchymal Stem Cells in a Model of Renal Artery Stenosis.Farooqui N, Mohan A, Isik B, Goksu BB, Thaler R, Zhu XY, Krier JD, Saadiq IM, Ferguson CM, Jordan KL, Tang H, Textor SC, Hickson TJ, van Wijnen AJ, Eirin A, Lerman LO, Herrmann SM. Farooqui N, et al. Stem Cells. 2023 Jan 30;41(1):50-63. doi: 10.1093/stmcls/sxac073. Stem Cells. 2023. PMID: 36250949
- Research Support, N.I.H., Extramural
- Acute Kidney Injury / therapy*
- Animals
- Antigens, CD34
- Apoptosis
- Caspase 3 / metabolism
- Cell- and Tissue-Based Therapy
- Cells, Cultured
- Endoplasmic Reticulum Stress
- Endothelial Cells / metabolism*
- Glomerular Filtration Rate
- Hyaluronan Receptors
- Kidney / blood supply
- Kidney / metabolism
- Kidney / pathology
- Mesenchymal Stem Cells / metabolism*
- Oxidative Stress
- Renal Artery Obstruction / metabolism*
- Renal Artery Obstruction / pathology
- Renal Circulation
- Swine
- Thy-1 Antigens
- Antigens, CD34
- Hyaluronan Receptors
- Thy-1 Antigens
- Caspase 3
- Full Text Sources
- Medical
- Research Materials
- Miscellaneous
NCBI Literature Resources
The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Unauthorized use of these marks is strictly prohibited.
National Library of Medicine
8600 Rockville Pike
Bethesda, MD 20894
![Figure 5](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3528811/bin/nihms420802f5.jpg)
Figure 6
Top: Western blotting images and…
Figure 6
Top: Western blotting images and quantification of the expression of CHOP and caspase-3…
- Adipose tissue-derived mesenchymal stem cells improve revascularization outcomes to restore renal function in swine atherosclerotic renal artery stenosis.Eirin A, Zhu XY, Krier JD, Tang H, Jordan KL, Grande JP, Lerman A, Textor SC, Lerman LO. Eirin A, et al. Stem Cells. 2012 May;30(5):1030-41. doi: 10.1002/stem.1047. Stem Cells. 2012. PMID: 22290832 Free PMC article.
- Mesenchymal Stem/Stromal Cells and their Extracellular Vesicle Progeny Decrease Injury in Poststenotic Swine Kidney Through Different Mechanisms.Zhao Y, Zhu X, Zhang L, Ferguson CM, Song T, Jiang K, Conley SM, Krier JD, Tang H, Saadiq I, Jordan KL, Lerman A, Lerman LO. Zhao Y, et al. Stem Cells Dev. 2020 Sep 15;29(18):1190-1200. doi: 10.1089/scd.2020.0030. Epub 2020 Aug 3. Stem Cells Dev. 2020. PMID: 32657229 Free PMC article.
- Adjunctive mesenchymal stem/stromal cells augment microvascular function in poststenotic kidneys treated with low-energy shockwave therapy.Chen XJ, Zhang X, Jiang K, Krier JD, Zhu X, Conley S, Lerman A, Lerman LO. Chen XJ, et al. J Cell Physiol. 2020 Dec;235(12):9806-9818. doi: 10.1002/jcp.29794. Epub 2020 May 19. J Cell Physiol. 2020. PMID: 32430932 Free PMC article.
- Addition of endothelial progenitor cells to renal revascularization restores medullary tubular oxygen consumption in swine renal artery stenosis.Ebrahimi B, Li Z, Eirin A, Zhu XY, Textor SC, Lerman LO. Ebrahimi B, et al. Am J Physiol Renal Physiol. 2012 Jun 1;302(11):F1478-85. doi: 10.1152/ajprenal.00563.2011. Epub 2012 Mar 14. Am J Physiol Renal Physiol. 2012. PMID: 22419692 Free PMC article.
- Stem cells in kidney diseases.Soler MJ, José Tomas OP. Soler MJ, et al. J Stem Cells. 2012;7(4):245-59. J Stem Cells. 2012. PMID: 24196799 Review.
- Immunological priming of mesenchymal stromal/stem cells and their extracellular vesicles augments their therapeutic benefits in experimental graft-versus-host disease via engagement of PD-1 ligands.Hackel A, Vollmer S, Bruderek K, Lang S, Brandau S. Hackel A, et al. Front Immunol. 2023 Feb 16;14:1078551. doi: 10.3389/fimmu.2023.1078551. eCollection 2023. Front Immunol. 2023. PMID: 36875112 Free PMC article.
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- Research Support, N.I.H., Extramural
- Acute Kidney Injury / therapy*
- Animals
- Antigens, CD34
- Apoptosis
- Caspase 3 / metabolism
- Cell- and Tissue-Based Therapy
- Cells, Cultured
- Endoplasmic Reticulum Stress
- Endothelial Cells / metabolism*
- Glomerular Filtration Rate
- Hyaluronan Receptors
- Kidney / blood supply
- Kidney / metabolism
- Kidney / pathology
- Mesenchymal Stem Cells / metabolism*
- Oxidative Stress
- Renal Artery Obstruction / metabolism*
- Renal Artery Obstruction / pathology
- Renal Circulation
- Swine
- Thy-1 Antigens
- Antigens, CD34
- Hyaluronan Receptors
- Thy-1 Antigens
- Caspase 3
- Full Text Sources
- Medical
- Research Materials
- Miscellaneous
![Figure 6](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/3528811/bin/nihms420802f6.jpg)
Source: PubMed