Emerging WuHan (COVID-19) coronavirus: glycan shield and structure prediction of spike glycoprotein and its interaction with human CD26
Naveen Vankadari, Jacqueline A Wilce, Naveen Vankadari, Jacqueline A Wilce
Abstract
The recent outbreak of pneumonia-causing COVID-19 in China is an urgent global public health issue with an increase in mortality and morbidity. Here we report our modelled homo-trimer structure of COVID-19 spike glycoprotein in both closed (ligand-free) and open (ligand-bound) conformation, which is involved in host cell adhesion. We also predict the unique N- and O-linked glycosylation sites of spike glycoprotein that distinguish it from the SARS and underlines shielding and camouflage of COVID-19 from the host the defence system. Furthermore, our study also highlights the key finding that the S1 domain of COVID-19 spike glycoprotein potentially interacts with the human CD26, a key immunoregulatory factor for hijacking and virulence. These findings accentuate the unique features of COVID-19 and assist in the development of new therapeutics.
Keywords: CD26; Coronavirus; docking; glycosylation; spike glycoprotein.
Conflict of interest statement
No potential conflict of interest was reported by the author(s).
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References
- Huang C, Wang Y, Li X, et al. . Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. Lancet. 2020;395(10223):497–506. doi: 10.1016/S0140-6736(20)30183-5
- Lu H, Stratton CW, Tang YW.. Outbreak of pneumonia of unknown etiology in Wuhan China: the mystery and the miracle. J Med Virol. 2020;92(4):401–402. doi: 10.1002/jmv.25678
- Xiong XL, Tortorici MA, Snijder J, et al. . Glycan shield and fusion activation of a deltacoronavirus spike glycoprotein fine-tuned for enteric infections. J Virol. 2018;92(4):e01628–17.
- Song WF, Gui M, Wang X, et al. . Cryo-EM structure of the SARS coronavirus spike glycoprotein in complex with its host cell receptor ACE2. PLoS Pathog. 2018;14(8):e1007236. doi: 10.1371/journal.ppat.1007236
- Millet JK, Kien F, Cheung C-Y, et al. . Ezrin interacts with the SARS coronavirus spike protein and restrains infection at the entry stage. Plos One. 2012;7(11): e49566. doi: 10.1371/journal.pone.0049566
- Xu X, Chen P, Wang J, et al. . Evolution of the novel coronavirus from the ongoing Wuhan outbreak and modeling of its spike protein for risk of human transmission. SCIENCE CHINA Life Sciences; 2020;63(3):457–460.
- Grum-Tokars V, Ratia K, Begaye A, et al. . Evaluating the 3C-like protease activity of SARS-coronavirus: recommendations for standardized assays for drug discovery. Virus Res. 2008;133(1):63–73. doi: 10.1016/j.virusres.2007.02.015
- Watanabe Y, Bowden TA, Wilson IA, et al. . Exploitation of glycosylation in enveloped virus pathobiology. Biochim Biophys Acta Gen Subj. 2019;1863(10):1480–1497. doi: 10.1016/j.bbagen.2019.05.012
- Morimoto C, Schlossman SF.. The structure and function of CD26 in the T-cell immune response. Immunol Rev. 1998;161:55–70. doi: 10.1111/j.1600-065X.1998.tb01571.x
- Wang Q, Qi J, Yuan Y, et al. . Bat origins of MERS-CoV supported by bat coronavirus HKU4 usage of human receptor CD26. Cell Host Microbe. 2014;16(3):328–337. doi: 10.1016/j.chom.2014.08.009
Source: PubMed