Evaluation of the anti-inflammatory activities of Quillaja saponaria Mol. saponin extract in mice

Sumana Sarkhel, Sumana Sarkhel

Abstract

Objective: Quillaja saponaria bark contains a high percentage of triterpene saponins and has been used for centuries as antiinflammatory and analgesic agent in Chilean folk medicine. In the Present study the anti-inflammatory activities of the aqueous extract of commercially partially purified saponin from Quillaja saponaria Mol. in in vivo animal models.

Methods & materials: Aqueous extract of the plant material was prepared by cold maceration. The anti-inflammatory activity of a commercial Quillaja saponaria Mol. (QS) saponin extract was investigated by carragenan induced mice paw edema model for acute inflammation (Winter, 1962) [16].

Results: The anti-inflammatory activity was evaluated by carragenan in paw edema model in swiss albino mice (18-20 g). The anti-inflammatory activity was found to be dose dependent in carragenan induced paw edema. QS was found to significantly (p < 0.05) reduce the carragenan induced mice paw edema (38.59%; 20 mg/kg bw) as compared to carragenan control. The percentage inhibition of standard anti-inflammatory drug indomethacin was (55%; 10 mg/kg, bw).

Conclusion: The results of the present study demonstrate that the aqueous extract of Quillaja saponaria saponins (QS) possess significant anti-inflammatory activity.

Keywords: Anti-inflammatory activity; Aqueous extract; Paw edema.

References

    1. Ferrero-Miliani L., Nielsen O.H., Andersen P.S., Girardin S.E. Chronic inflammation: importance of NOD2 and NALP3 in interleukin-1beta generation. Clin. Exp. Immunol. 2007;147(2):227–235.
    1. Dassoler M., Schwanz M., Busseto F., Moreira E.A., Gutierrez L. Perfil fitoquímico e ensaiofarmacológico de Averrhoa carambola L. (Oxalidaceae) Jornal Brasileiro de Fitomedicina. 2004;2:4–8.
    1. Hostettmann K., Marston A. Cambridge University Press; Cambridge, New York: 1995. Saponins.
    1. Milgate J., Roberts D.C.K. The nutritional and biological significance of saponins. Nutr. Res. 1995;15:1223–1249.
    1. Lacaille-Dubois M.A., Wagner H. A review of the biological and pharmacologicalactivities of saponins. Phytomedicine. 1996;2:363–386.
    1. Francis G., Kerem Z., Makkar H.P.S., Becker K. The biological action of saponins in animal systems: a review. Br. J. Nutr. 2002;88:587–605.
    1. De La Lastra C.A., Villegas I. Resveratrol as an anti-inflammatory and anti- ageing agent: mechanism & clinical implications. Mol. Nutr. Food Res. 2005;49(5):405–430.
    1. Liu Y., Song M., Che T.M., Bravo D., Pettigraw J.E. Anti-inflammatory effects of several plant extracts on porcine alveolar macrophage in vitro. J. Anim. Sci. 2012;90(8):2774–2783.
    1. Lee K.H., Kim A.J., Choi E.M. Antioxidant and anti-inflammatory activity of pine pollen extract in vitro. Phytother. Res. 2009;23(1):41–48.
    1. Kang J.S., Lee K.H., Han M.H., Lee H., Ahn J.M., Han S.B., Lee K., Park S.K., Kim H.M. Anti-inflammatory activity of methanol extract isolated from stem bark of Magnolia kobus. Phytother. Res. 2008;22(7):883–888.
    1. Kubitzki K. vol. 9. Springer; Berlin & Heidelberg, New York: 2007. pp. 407–408. (The Families and Genera of Vascular Plants).
    1. Sun H.X., Xie Y., Ye Y.P. Advances in saponin based adjuvants. Vaccines. 2009;27(12):1787–1796.
    1. J. Zin, Weiss C. La Salud Por Medio de Las Plantas Medicinales, 6th ed., Editorial Salesiana, Santiago de Chile; 1980, p. 277. (in Spanish)
    1. Roner M.R., Sprayberry J., Spinks M., Dhanji S. Antiviral activity obtainedfrom aqueous extracts of the Chilean soap bark tree (Quillaja saponaria Mol.) J. Genet. Virol. 2007;88:275–285.
    1. Bruce R.D. An up and down procedure for acute toxicity testing. Fundam. Appl. Toxicol. 1995;5:151–157.
    1. Winter C.A., Risley E.A., Nuss G.W. Carrageenan-induced oedemain the hind paw of rat as an assay for anti-inflammatory activity. Proc. Soc. Exp. Biol. Ther. 1962;111:544–547.
    1. Vinegar R., Schreiber W., Hugo R. Biphasic development of carrageenan in rats. Pharmacol. Exp. Ther. 1969;166:96–103.
    1. Seibert K., Masferrer J.L. Role of inducible cycloxygenase (COX 2) in inflammation. Receptor. 1994;4(1):17–23.
    1. Rodríguez-Díaz M., Delporte C., Cassels B.K., González P., Silva X., León F. Topical anti-inflammatory activity of quillaic acid from Quillaja saponaria Mol. and some derivatives. J. Pharm. Pharmacol. 2011;63(5):718–724.
    1. Arrau S., Delporte C., Cartagena C., Roddriquez-Diaz M., Gonzalez P., Silva X., Casssels B.K., Miranda H.F. Antinociceptive activity of Quillaja saponaria Mol. saponin extract, quallic acid and derivatives in mice. J. Ethnopharmacol. 2011;133(1):164–167.

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