An Absorption and Plasma Kinetics Study of Monoterpenes Present in Mastiha Oil in Humans

Efstathia Papada, Aristea Gioxari, Charalampia Amerikanou, Nikolaos Galanis, Andriana C Kaliora, Efstathia Papada, Aristea Gioxari, Charalampia Amerikanou, Nikolaos Galanis, Andriana C Kaliora

Abstract

Monoterpenes are bioactive compounds, however studies on their metabolic fate in humans are scarce. The present work aimed to identify and quantify the bioactive monoterpenes myrcene, α- and β-pinene of the Mediterranean product Mastiha Oil, in human plasma after acute consumption of a single dose. This was an open-label, single-arm acute study. After overnight fasting, healthy males were administered with Mastiha Oil. Blood samples were collected on different time-points before and after consumption. A novel GC-MS-MS application was performed to detect and quantify terpenes in MO and in plasma. Serum lipid resistance to oxidation was also determined. Alpha-Pinene, β-pinene and myrcene were identified and quantified in plasma. Alpha-pinene concentration significantly increased after 0.5 h of Mastiha Oil consumption, remaining significantly increased at 1 h, 2 h, 4 h, 6 h and 24 h. Beta-pinene and myrcene followed similar patterns. The increase in serum lipid resistance to oxidation was significant at 1 h, reached its peak at 2 h and remained significant until 4 h. Conclusively, α-pinene, β-pinene and myrcene that are present in Mastiha Oil are absorbed by humans. (ClinicalTrials.gov Identifier: NCT04290312).

Keywords: absorption; mastiha oil; monoterpenes.

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Study flowchart.
Figure 2
Figure 2
Plasma concentration-time curves for β-pinene, myrcene and a-pinene. Values are presented as mean ± standard error of mean. * p <0.05.
Figure 2
Figure 2
Plasma concentration-time curves for β-pinene, myrcene and a-pinene. Values are presented as mean ± standard error of mean. * p <0.05.
Figure 3
Figure 3
Antioxidant capacity was estimated applying the copper sulphate oxidation assay. * p < 0.05.

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