TRIM59 facilitates the proliferation of colorectal cancer and promotes metastasis via the PI3K/AKT pathway
Ye Sun, Bing Ji, Yifei Feng, Yue Zhang, Dongjian Ji, Chunyan Zhu, Sen Wang, Chuan Zhang, Dongsheng Zhang, Yueming Sun, Ye Sun, Bing Ji, Yifei Feng, Yue Zhang, Dongjian Ji, Chunyan Zhu, Sen Wang, Chuan Zhang, Dongsheng Zhang, Yueming Sun
Abstract
Tripartite motif-containing 59 (TRIM59) belongs to the tripartite motif (TRIM) protein family and is upregulated in various malignancies. However, its expression in colorectal cancer (CRC) is still unknown. In the present study, we examined the expression and biological function of TRIM59 in CRC. We analyzed CRC tissues and cells by quantitative real-time polymerase chain reaction. Kaplan-Meier survival analysis was used to evaluate the prognostic significance of TRIM59 in CRC patients. Furthermore, we investigated the role of TRIM59 in CRC growth and metastasis. The potential mechanism underlying the regulation of cell metastasis by TRIM59 was determined by western blotting. TRIM59 expression was conspicuously overexpressed in CRC tissues and CRC cell lines compared to that noted in the corresponding normal control cells. Patients with higher TRIM59 expression had poorer prognosis. Furthermore, knockdown of TRIM59 suppressed cell proliferation through the induction of apoptosis and inhibited migration and invasion significantly in vitro. Further investigation revealed that knockdown of TRIM59 effectively reversed the expression of epithelial-mesenchymal transformation‑related proteins vimentin, Snail and E-cadherin. Our preliminary results confirm that TRIM59 can be mediated by PI3K/AKT signaling. TRIM59 functions as an oncogene in CRC progression, which could be a novel target for the detection and treatment of CRC.
Figures
TRIM59 is upregulated in human CRC tissues and cell lines. (A) TRIM59 mRNA levels in CRC and normal tissues (n=90) were assessed by real-time polymerase chain reaction (***p Figure 2.
Figure 2.
TRIM59 expression is suppressed by…
Figure 2.
TRIM59 expression is suppressed by TRIM59 siRNA transfection. (A) The levels of TRIM59…
TRIM59 expression is suppressed by TRIM59 siRNA transfection. (A) The levels of TRIM59 mRNA were verified by RT-PCR in LoVo and DLD-1 cells after the treatment with siRNAs against TRIM59; *p Figure 3. Figure 4.
Figure 3.
Effect of TRIM59 on the…
Figure 3.
Effect of TRIM59 on the regulation of cell proliferation. (A and B) The…
Effect of TRIM59 on the regulation of cell proliferation. (A and B) The CCK-8 assay showed that silencing of TRIM59 inhibited LoVo and DLD-1 cell proliferation. (C and D) The colony-forming ability of TRIM59-knockdown cells compared with NC. The colony number was normalized to cells without any treatment, which was set to 100%. Data are presented as mean ± standard deviation from three independent experiments; *p<0.05.
Figure 4.
Knockdown of TRIM59 increases the…
Figure 4.
Knockdown of TRIM59 increases the apoptosis of CRC cells. Apoptosis of (A) LoVo…
Knockdown of TRIM59 increases the apoptosis of CRC cells. Apoptosis of (A) LoVo and (B) DLD-1 cell lines was detected using flow cytometry; *p Figure 5.
Figure 5.
Knockdown of TRIM59 inhibits the…
Figure 5.
Knockdown of TRIM59 inhibits the migration and invasion of CRC cells. (A) Wound…
Knockdown of TRIM59 inhibits the migration and invasion of CRC cells. (A) Wound healing assay was performed on untransfected cells (blank), cells transfected with control siRNA (NC) and cells transfected with TRIM59 siRNA3 (siRNA3). Quantitative results are shown. Magnification, ×40. (B) Migration and invasion through the Transwell assay. Numbers of migrated cells through the membrane and invaded cells through Matrigel of each cell line are shown. Magnification, ×100. (C and D) The effect of TRIM59 silencing on metastasis-related genes was performed by detecting mRNA and protein levels. Each assay is performed in triplicates. Data are presented as mean ± standard deviation; *p Figure 6.
Figure 6.
TRIM59 knockdown suppresses the activation…
Figure 6.
TRIM59 knockdown suppresses the activation of the PI3K/AKT pathways in CRC cells. Expression…
TRIM59 knockdown suppresses the activation of the PI3K/AKT pathways in CRC cells. Expression of PI3K, p-PI3K, AKT, p-AKT was evaluated by western blotting in (A) LoVo and (B) DLD-1 cells. (C) The relative protein expression levels of p-PI3K and p-AKT were quantified in the two cell lines. Each assay is performed in triplicate. Data are presented as mean ± standard deviation; *p Figure 7.
Figure 7.
Overexpression of TRIM59 reduces the…
Figure 7.
Overexpression of TRIM59 reduces the effects of the AKT inhibitor on the migration…
Overexpression of TRIM59 reduces the effects of the AKT inhibitor on the migration and invasion of CRC cells. (A) The levels of TRIM59 mRNA were verified by RT-PCR in the NCM460 cell line after treatment with the TRIM59 plasmid. (B) TRIM59 protein levels were determined using western blotting and quantification is illustrated by a bar chart. (C) Cells were transfected with control plasmid (vehicle) or TRIM59 plasmid and treated with LY294002 (20 µM) for 24 h, following which, migration and invasion assays were performed in these cells. Dimethyl sulfoxide was used as a negative control; **p
All figures (7)
Similar articles
- Knockdown of tripartite motif 59 (TRIM59) inhibits proliferation in cholangiocarcinoma via the PI3K/AKT/mTOR signalling pathway.Shen H, Zhang J, Zhang Y, Feng Q, Wang H, Li G, Jiang W, Li X. Shen H, et al. Gene. 2019 May 25;698:50-60. doi: 10.1016/j.gene.2019.02.044. Epub 2019 Feb 27. Gene. 2019. PMID: 30822475
- GPR56 promotes proliferation of colorectal cancer cells and enhances metastasis via epithelial‑mesenchymal transition through PI3K/AKT signaling activation.Ji B, Feng Y, Sun Y, Ji D, Qian W, Zhang Z, Wang Q, Zhang Y, Zhang C, Sun Y. Ji B, et al. Oncol Rep. 2018 Oct;40(4):1885-1896. doi: 10.3892/or.2018.6582. Epub 2018 Jul 20. Oncol Rep. 2018. PMID: 30066935 Free PMC article.
- Tripartite Motif-Containing Protein 59 (TRIM59) Promotes Epithelial Ovarian Cancer Progression via the Focal Adhesion Kinase(FAK)/AKT/Matrix Metalloproteinase (MMP) Pathway.Zhang P, Zhang H, Wang Y, Zhang P, Qi Y. Zhang P, et al. Med Sci Monit. 2019 May 7;25:3366-3373. doi: 10.12659/MSM.916299. Med Sci Monit. 2019. PMID: 31062766 Free PMC article.
- The role of Tripartite motif containing 59 (TRIM59) in the proliferation and prognosis of intrahepatic cholangiocarcinoma.Zhang JN, Ding DY, Yang SY, Tao QF, Yang Y, Zhou WP. Zhang JN, et al. Pathol Res Pract. 2022 Aug;236:153989. doi: 10.1016/j.prp.2022.153989. Epub 2022 Jun 17. Pathol Res Pract. 2022. PMID: 35753134 Review.
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Cited by
- Targeting KRAS G12C mutations in colorectal cancer.Zhao MH, Wu AW. Zhao MH, et al. Gastroenterol Rep (Oxf). 2022 Dec 30;11:goac083. doi: 10.1093/gastro/goac083. eCollection 2023. Gastroenterol Rep (Oxf). 2022. PMID: 36632627 Free PMC article. Review.
- TRIM59 guards ER proteostasis and prevents Bortezomib-mediated colorectal cancer (CRC) cells' killing.Feng X, Yang G, Zhang L, Tao S, Shim JS, Chen L, Wu Q. Feng X, et al. Invest New Drugs. 2022 Dec;40(6):1244-1253. doi: 10.1007/s10637-022-01306-7. Epub 2022 Oct 28. Invest New Drugs. 2022. PMID: 36306030
- TRIM family contribute to tumorigenesis, cancer development, and drug resistance.Huang N, Sun X, Li P, Liu X, Zhang X, Chen Q, Xin H. Huang N, et al. Exp Hematol Oncol. 2022 Oct 19;11(1):75. doi: 10.1186/s40164-022-00322-w. Exp Hematol Oncol. 2022. PMID: 36261847 Free PMC article. Review.
- The roles and targeting options of TRIM family proteins in tumor.Zhang Y, Zhang W, Zheng L, Guo Q. Zhang Y, et al. Front Pharmacol. 2022 Sep 30;13:999380. doi: 10.3389/fphar.2022.999380. eCollection 2022. Front Pharmacol. 2022. PMID: 36249749 Free PMC article. Review.
- Prognostic Factors of Colorectal Cancer: A Comparative Study on Patients With or Without Liver Metastasis.Peng H, Liu G, Bao Y, Zhang X, Zhou L, Huang C, Song Z, Cao S, Dang S, Zhang J, Huang T, Wu Y, Xu M, Song L, Cao P. Peng H, et al. Front Oncol. 2021 Dec 21;11:626190. doi: 10.3389/fonc.2021.626190. eCollection 2021. Front Oncol. 2021. PMID: 34993129 Free PMC article.
References
-
- Yamada Y, Takayama KI, Fujimura T, Ashikari D, Obinata D, Takahashi S, Ikeda K, Kakutani S, Urano T, Fukuhara H, et al. A novel prognostic factor TRIM44 promotes cell proliferation and migration, and inhibits apoptosis in testicular germ cell tumor. Cancer Sci. 2017;108:32–41. doi: 10.1111/cas.13105. - DOI - PMC - PubMed
Show all 27 references
MeSH terms
- Apoptosis
- Biomarkers, Tumor / metabolism
- Cell Movement*
- Cell Proliferation*
- Colorectal Neoplasms / metabolism
- Colorectal Neoplasms / secondary*
- Epithelial-Mesenchymal Transition
- Female
- Gene Expression Regulation, Neoplastic*
- Humans
- Intracellular Signaling Peptides and Proteins
- Lymphatic Metastasis
- Male
- Membrane Proteins / metabolism*
- Metalloproteins / metabolism*
- Middle Aged
- Neoplasm Invasiveness
- Phosphatidylinositol 3-Kinases / metabolism*
- Prognosis
- Proto-Oncogene Proteins c-akt / metabolism*
- Signal Transduction
- Tripartite Motif Proteins
- Tumor Cells, Cultured
Substances
- Biomarkers, Tumor
- Intracellular Signaling Peptides and Proteins
- Membrane Proteins
- Metalloproteins
- TRIM59 protein, human
- Tripartite Motif Proteins
- Proto-Oncogene Proteins c-akt
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TRIM59 expression is suppressed by TRIM59 siRNA transfection. (A) The levels of TRIM59 mRNA were verified by RT-PCR in LoVo and DLD-1 cells after the treatment with siRNAs against TRIM59; *p Figure 3. Figure 4.
Figure 3.
Effect of TRIM59 on the…
Figure 3.
Effect of TRIM59 on the regulation of cell proliferation. (A and B) The…
Effect of TRIM59 on the regulation of cell proliferation. (A and B) The CCK-8 assay showed that silencing of TRIM59 inhibited LoVo and DLD-1 cell proliferation. (C and D) The colony-forming ability of TRIM59-knockdown cells compared with NC. The colony number was normalized to cells without any treatment, which was set to 100%. Data are presented as mean ± standard deviation from three independent experiments; *p<0.05.
Figure 4.
Knockdown of TRIM59 increases the…
Figure 4.
Knockdown of TRIM59 increases the apoptosis of CRC cells. Apoptosis of (A) LoVo…
Knockdown of TRIM59 increases the apoptosis of CRC cells. Apoptosis of (A) LoVo and (B) DLD-1 cell lines was detected using flow cytometry; *p Figure 5.
Figure 5.
Knockdown of TRIM59 inhibits the…
Figure 5.
Knockdown of TRIM59 inhibits the migration and invasion of CRC cells. (A) Wound…
Knockdown of TRIM59 inhibits the migration and invasion of CRC cells. (A) Wound healing assay was performed on untransfected cells (blank), cells transfected with control siRNA (NC) and cells transfected with TRIM59 siRNA3 (siRNA3). Quantitative results are shown. Magnification, ×40. (B) Migration and invasion through the Transwell assay. Numbers of migrated cells through the membrane and invaded cells through Matrigel of each cell line are shown. Magnification, ×100. (C and D) The effect of TRIM59 silencing on metastasis-related genes was performed by detecting mRNA and protein levels. Each assay is performed in triplicates. Data are presented as mean ± standard deviation; *p Figure 6.
Figure 6.
TRIM59 knockdown suppresses the activation…
Figure 6.
TRIM59 knockdown suppresses the activation of the PI3K/AKT pathways in CRC cells. Expression…
TRIM59 knockdown suppresses the activation of the PI3K/AKT pathways in CRC cells. Expression of PI3K, p-PI3K, AKT, p-AKT was evaluated by western blotting in (A) LoVo and (B) DLD-1 cells. (C) The relative protein expression levels of p-PI3K and p-AKT were quantified in the two cell lines. Each assay is performed in triplicate. Data are presented as mean ± standard deviation; *p Figure 7.
Figure 7.
Overexpression of TRIM59 reduces the…
Figure 7.
Overexpression of TRIM59 reduces the effects of the AKT inhibitor on the migration…
Overexpression of TRIM59 reduces the effects of the AKT inhibitor on the migration and invasion of CRC cells. (A) The levels of TRIM59 mRNA were verified by RT-PCR in the NCM460 cell line after treatment with the TRIM59 plasmid. (B) TRIM59 protein levels were determined using western blotting and quantification is illustrated by a bar chart. (C) Cells were transfected with control plasmid (vehicle) or TRIM59 plasmid and treated with LY294002 (20 µM) for 24 h, following which, migration and invasion assays were performed in these cells. Dimethyl sulfoxide was used as a negative control; **p
All figures (7)
Similar articles
- Knockdown of tripartite motif 59 (TRIM59) inhibits proliferation in cholangiocarcinoma via the PI3K/AKT/mTOR signalling pathway.Shen H, Zhang J, Zhang Y, Feng Q, Wang H, Li G, Jiang W, Li X. Shen H, et al. Gene. 2019 May 25;698:50-60. doi: 10.1016/j.gene.2019.02.044. Epub 2019 Feb 27. Gene. 2019. PMID: 30822475
- GPR56 promotes proliferation of colorectal cancer cells and enhances metastasis via epithelial‑mesenchymal transition through PI3K/AKT signaling activation.Ji B, Feng Y, Sun Y, Ji D, Qian W, Zhang Z, Wang Q, Zhang Y, Zhang C, Sun Y. Ji B, et al. Oncol Rep. 2018 Oct;40(4):1885-1896. doi: 10.3892/or.2018.6582. Epub 2018 Jul 20. Oncol Rep. 2018. PMID: 30066935 Free PMC article.
- Tripartite Motif-Containing Protein 59 (TRIM59) Promotes Epithelial Ovarian Cancer Progression via the Focal Adhesion Kinase(FAK)/AKT/Matrix Metalloproteinase (MMP) Pathway.Zhang P, Zhang H, Wang Y, Zhang P, Qi Y. Zhang P, et al. Med Sci Monit. 2019 May 7;25:3366-3373. doi: 10.12659/MSM.916299. Med Sci Monit. 2019. PMID: 31062766 Free PMC article.
- The role of Tripartite motif containing 59 (TRIM59) in the proliferation and prognosis of intrahepatic cholangiocarcinoma.Zhang JN, Ding DY, Yang SY, Tao QF, Yang Y, Zhou WP. Zhang JN, et al. Pathol Res Pract. 2022 Aug;236:153989. doi: 10.1016/j.prp.2022.153989. Epub 2022 Jun 17. Pathol Res Pract. 2022. PMID: 35753134 Review.
- Multifaceted Roles of TRIM Proteins in Colorectal Carcinoma.Eberhardt W, Haeussler K, Nasrullah U, Pfeilschifter J. Eberhardt W, et al. Int J Mol Sci. 2020 Oct 13;21(20):7532. doi: 10.3390/ijms21207532. Int J Mol Sci. 2020. PMID: 33066016 Free PMC article. Review.
Cited by
- Targeting KRAS G12C mutations in colorectal cancer.Zhao MH, Wu AW. Zhao MH, et al. Gastroenterol Rep (Oxf). 2022 Dec 30;11:goac083. doi: 10.1093/gastro/goac083. eCollection 2023. Gastroenterol Rep (Oxf). 2022. PMID: 36632627 Free PMC article. Review.
- TRIM59 guards ER proteostasis and prevents Bortezomib-mediated colorectal cancer (CRC) cells' killing.Feng X, Yang G, Zhang L, Tao S, Shim JS, Chen L, Wu Q. Feng X, et al. Invest New Drugs. 2022 Dec;40(6):1244-1253. doi: 10.1007/s10637-022-01306-7. Epub 2022 Oct 28. Invest New Drugs. 2022. PMID: 36306030
- TRIM family contribute to tumorigenesis, cancer development, and drug resistance.Huang N, Sun X, Li P, Liu X, Zhang X, Chen Q, Xin H. Huang N, et al. Exp Hematol Oncol. 2022 Oct 19;11(1):75. doi: 10.1186/s40164-022-00322-w. Exp Hematol Oncol. 2022. PMID: 36261847 Free PMC article. Review.
- The roles and targeting options of TRIM family proteins in tumor.Zhang Y, Zhang W, Zheng L, Guo Q. Zhang Y, et al. Front Pharmacol. 2022 Sep 30;13:999380. doi: 10.3389/fphar.2022.999380. eCollection 2022. Front Pharmacol. 2022. PMID: 36249749 Free PMC article. Review.
- Prognostic Factors of Colorectal Cancer: A Comparative Study on Patients With or Without Liver Metastasis.Peng H, Liu G, Bao Y, Zhang X, Zhou L, Huang C, Song Z, Cao S, Dang S, Zhang J, Huang T, Wu Y, Xu M, Song L, Cao P. Peng H, et al. Front Oncol. 2021 Dec 21;11:626190. doi: 10.3389/fonc.2021.626190. eCollection 2021. Front Oncol. 2021. PMID: 34993129 Free PMC article.
References
-
- Yamada Y, Takayama KI, Fujimura T, Ashikari D, Obinata D, Takahashi S, Ikeda K, Kakutani S, Urano T, Fukuhara H, et al. A novel prognostic factor TRIM44 promotes cell proliferation and migration, and inhibits apoptosis in testicular germ cell tumor. Cancer Sci. 2017;108:32–41. doi: 10.1111/cas.13105. - DOI - PMC - PubMed
Show all 27 references
MeSH terms
- Apoptosis
- Biomarkers, Tumor / metabolism
- Cell Movement*
- Cell Proliferation*
- Colorectal Neoplasms / metabolism
- Colorectal Neoplasms / secondary*
- Epithelial-Mesenchymal Transition
- Female
- Gene Expression Regulation, Neoplastic*
- Humans
- Intracellular Signaling Peptides and Proteins
- Lymphatic Metastasis
- Male
- Membrane Proteins / metabolism*
- Metalloproteins / metabolism*
- Middle Aged
- Neoplasm Invasiveness
- Phosphatidylinositol 3-Kinases / metabolism*
- Prognosis
- Proto-Oncogene Proteins c-akt / metabolism*
- Signal Transduction
- Tripartite Motif Proteins
- Tumor Cells, Cultured
Substances
- Biomarkers, Tumor
- Intracellular Signaling Peptides and Proteins
- Membrane Proteins
- Metalloproteins
- TRIM59 protein, human
- Tripartite Motif Proteins
- Proto-Oncogene Proteins c-akt
Related information
LinkOut - more resources
- Full Text Sources
- Other Literature Sources
- Medical
- Research Materials
Full text links [x]
[x]
Cite
Copy Download .nbib
Format: AMA APA MLA NLM
Send To [x]
NCBI Literature Resources
The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Unauthorized use of these marks is strictly prohibited.
Follow NCBI
National Library of Medicine
8600 Rockville Pike
Bethesda, MD 20894
Effect of TRIM59 on the regulation of cell proliferation. (A and B) The CCK-8 assay showed that silencing of TRIM59 inhibited LoVo and DLD-1 cell proliferation. (C and D) The colony-forming ability of TRIM59-knockdown cells compared with NC. The colony number was normalized to cells without any treatment, which was set to 100%. Data are presented as mean ± standard deviation from three independent experiments; *p<0.05.
Knockdown of TRIM59 increases the apoptosis of CRC cells. Apoptosis of (A) LoVo and (B) DLD-1 cell lines was detected using flow cytometry; *p Figure 5.
Figure 5.
Knockdown of TRIM59 inhibits the…
Figure 5.
Knockdown of TRIM59 inhibits the migration and invasion of CRC cells. (A) Wound…
Knockdown of TRIM59 inhibits the migration and invasion of CRC cells. (A) Wound healing assay was performed on untransfected cells (blank), cells transfected with control siRNA (NC) and cells transfected with TRIM59 siRNA3 (siRNA3). Quantitative results are shown. Magnification, ×40. (B) Migration and invasion through the Transwell assay. Numbers of migrated cells through the membrane and invaded cells through Matrigel of each cell line are shown. Magnification, ×100. (C and D) The effect of TRIM59 silencing on metastasis-related genes was performed by detecting mRNA and protein levels. Each assay is performed in triplicates. Data are presented as mean ± standard deviation; *p Figure 6.
Figure 6.
TRIM59 knockdown suppresses the activation…
Figure 6.
TRIM59 knockdown suppresses the activation of the PI3K/AKT pathways in CRC cells. Expression…
TRIM59 knockdown suppresses the activation of the PI3K/AKT pathways in CRC cells. Expression of PI3K, p-PI3K, AKT, p-AKT was evaluated by western blotting in (A) LoVo and (B) DLD-1 cells. (C) The relative protein expression levels of p-PI3K and p-AKT were quantified in the two cell lines. Each assay is performed in triplicate. Data are presented as mean ± standard deviation; *p Figure 7.
Figure 7.
Overexpression of TRIM59 reduces the…
Figure 7.
Overexpression of TRIM59 reduces the effects of the AKT inhibitor on the migration…
Overexpression of TRIM59 reduces the effects of the AKT inhibitor on the migration and invasion of CRC cells. (A) The levels of TRIM59 mRNA were verified by RT-PCR in the NCM460 cell line after treatment with the TRIM59 plasmid. (B) TRIM59 protein levels were determined using western blotting and quantification is illustrated by a bar chart. (C) Cells were transfected with control plasmid (vehicle) or TRIM59 plasmid and treated with LY294002 (20 µM) for 24 h, following which, migration and invasion assays were performed in these cells. Dimethyl sulfoxide was used as a negative control; **p
All figures (7)
Similar articles
- Knockdown of tripartite motif 59 (TRIM59) inhibits proliferation in cholangiocarcinoma via the PI3K/AKT/mTOR signalling pathway.Shen H, Zhang J, Zhang Y, Feng Q, Wang H, Li G, Jiang W, Li X. Shen H, et al. Gene. 2019 May 25;698:50-60. doi: 10.1016/j.gene.2019.02.044. Epub 2019 Feb 27. Gene. 2019. PMID: 30822475
- GPR56 promotes proliferation of colorectal cancer cells and enhances metastasis via epithelial‑mesenchymal transition through PI3K/AKT signaling activation.Ji B, Feng Y, Sun Y, Ji D, Qian W, Zhang Z, Wang Q, Zhang Y, Zhang C, Sun Y. Ji B, et al. Oncol Rep. 2018 Oct;40(4):1885-1896. doi: 10.3892/or.2018.6582. Epub 2018 Jul 20. Oncol Rep. 2018. PMID: 30066935 Free PMC article.
- Tripartite Motif-Containing Protein 59 (TRIM59) Promotes Epithelial Ovarian Cancer Progression via the Focal Adhesion Kinase(FAK)/AKT/Matrix Metalloproteinase (MMP) Pathway.Zhang P, Zhang H, Wang Y, Zhang P, Qi Y. Zhang P, et al. Med Sci Monit. 2019 May 7;25:3366-3373. doi: 10.12659/MSM.916299. Med Sci Monit. 2019. PMID: 31062766 Free PMC article.
- The role of Tripartite motif containing 59 (TRIM59) in the proliferation and prognosis of intrahepatic cholangiocarcinoma.Zhang JN, Ding DY, Yang SY, Tao QF, Yang Y, Zhou WP. Zhang JN, et al. Pathol Res Pract. 2022 Aug;236:153989. doi: 10.1016/j.prp.2022.153989. Epub 2022 Jun 17. Pathol Res Pract. 2022. PMID: 35753134 Review.
- Multifaceted Roles of TRIM Proteins in Colorectal Carcinoma.Eberhardt W, Haeussler K, Nasrullah U, Pfeilschifter J. Eberhardt W, et al. Int J Mol Sci. 2020 Oct 13;21(20):7532. doi: 10.3390/ijms21207532. Int J Mol Sci. 2020. PMID: 33066016 Free PMC article. Review.
Cited by
- Targeting KRAS G12C mutations in colorectal cancer.Zhao MH, Wu AW. Zhao MH, et al. Gastroenterol Rep (Oxf). 2022 Dec 30;11:goac083. doi: 10.1093/gastro/goac083. eCollection 2023. Gastroenterol Rep (Oxf). 2022. PMID: 36632627 Free PMC article. Review.
- TRIM59 guards ER proteostasis and prevents Bortezomib-mediated colorectal cancer (CRC) cells' killing.Feng X, Yang G, Zhang L, Tao S, Shim JS, Chen L, Wu Q. Feng X, et al. Invest New Drugs. 2022 Dec;40(6):1244-1253. doi: 10.1007/s10637-022-01306-7. Epub 2022 Oct 28. Invest New Drugs. 2022. PMID: 36306030
- TRIM family contribute to tumorigenesis, cancer development, and drug resistance.Huang N, Sun X, Li P, Liu X, Zhang X, Chen Q, Xin H. Huang N, et al. Exp Hematol Oncol. 2022 Oct 19;11(1):75. doi: 10.1186/s40164-022-00322-w. Exp Hematol Oncol. 2022. PMID: 36261847 Free PMC article. Review.
- The roles and targeting options of TRIM family proteins in tumor.Zhang Y, Zhang W, Zheng L, Guo Q. Zhang Y, et al. Front Pharmacol. 2022 Sep 30;13:999380. doi: 10.3389/fphar.2022.999380. eCollection 2022. Front Pharmacol. 2022. PMID: 36249749 Free PMC article. Review.
- Prognostic Factors of Colorectal Cancer: A Comparative Study on Patients With or Without Liver Metastasis.Peng H, Liu G, Bao Y, Zhang X, Zhou L, Huang C, Song Z, Cao S, Dang S, Zhang J, Huang T, Wu Y, Xu M, Song L, Cao P. Peng H, et al. Front Oncol. 2021 Dec 21;11:626190. doi: 10.3389/fonc.2021.626190. eCollection 2021. Front Oncol. 2021. PMID: 34993129 Free PMC article.
References
-
- Yamada Y, Takayama KI, Fujimura T, Ashikari D, Obinata D, Takahashi S, Ikeda K, Kakutani S, Urano T, Fukuhara H, et al. A novel prognostic factor TRIM44 promotes cell proliferation and migration, and inhibits apoptosis in testicular germ cell tumor. Cancer Sci. 2017;108:32–41. doi: 10.1111/cas.13105. - DOI - PMC - PubMed
Show all 27 references
MeSH terms
- Apoptosis
- Biomarkers, Tumor / metabolism
- Cell Movement*
- Cell Proliferation*
- Colorectal Neoplasms / metabolism
- Colorectal Neoplasms / secondary*
- Epithelial-Mesenchymal Transition
- Female
- Gene Expression Regulation, Neoplastic*
- Humans
- Intracellular Signaling Peptides and Proteins
- Lymphatic Metastasis
- Male
- Membrane Proteins / metabolism*
- Metalloproteins / metabolism*
- Middle Aged
- Neoplasm Invasiveness
- Phosphatidylinositol 3-Kinases / metabolism*
- Prognosis
- Proto-Oncogene Proteins c-akt / metabolism*
- Signal Transduction
- Tripartite Motif Proteins
- Tumor Cells, Cultured
Substances
- Biomarkers, Tumor
- Intracellular Signaling Peptides and Proteins
- Membrane Proteins
- Metalloproteins
- TRIM59 protein, human
- Tripartite Motif Proteins
- Proto-Oncogene Proteins c-akt
Related information
LinkOut - more resources
- Full Text Sources
- Other Literature Sources
- Medical
- Research Materials
Full text links [x]
[x]
Cite
Copy Download .nbib
Format: AMA APA MLA NLM
Send To [x]
NCBI Literature Resources
The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Unauthorized use of these marks is strictly prohibited.
Follow NCBI
National Library of Medicine
8600 Rockville Pike
Bethesda, MD 20894
Knockdown of TRIM59 inhibits the migration and invasion of CRC cells. (A) Wound healing assay was performed on untransfected cells (blank), cells transfected with control siRNA (NC) and cells transfected with TRIM59 siRNA3 (siRNA3). Quantitative results are shown. Magnification, ×40. (B) Migration and invasion through the Transwell assay. Numbers of migrated cells through the membrane and invaded cells through Matrigel of each cell line are shown. Magnification, ×100. (C and D) The effect of TRIM59 silencing on metastasis-related genes was performed by detecting mRNA and protein levels. Each assay is performed in triplicates. Data are presented as mean ± standard deviation; *p Figure 6.
Figure 6.
TRIM59 knockdown suppresses the activation…
Figure 6.
TRIM59 knockdown suppresses the activation of the PI3K/AKT pathways in CRC cells. Expression…
TRIM59 knockdown suppresses the activation of the PI3K/AKT pathways in CRC cells. Expression of PI3K, p-PI3K, AKT, p-AKT was evaluated by western blotting in (A) LoVo and (B) DLD-1 cells. (C) The relative protein expression levels of p-PI3K and p-AKT were quantified in the two cell lines. Each assay is performed in triplicate. Data are presented as mean ± standard deviation; *p Figure 7.
Figure 7.
Overexpression of TRIM59 reduces the…
Figure 7.
Overexpression of TRIM59 reduces the effects of the AKT inhibitor on the migration…
Overexpression of TRIM59 reduces the effects of the AKT inhibitor on the migration and invasion of CRC cells. (A) The levels of TRIM59 mRNA were verified by RT-PCR in the NCM460 cell line after treatment with the TRIM59 plasmid. (B) TRIM59 protein levels were determined using western blotting and quantification is illustrated by a bar chart. (C) Cells were transfected with control plasmid (vehicle) or TRIM59 plasmid and treated with LY294002 (20 µM) for 24 h, following which, migration and invasion assays were performed in these cells. Dimethyl sulfoxide was used as a negative control; **p
All figures (7)
Similar articles
- Knockdown of tripartite motif 59 (TRIM59) inhibits proliferation in cholangiocarcinoma via the PI3K/AKT/mTOR signalling pathway.Shen H, Zhang J, Zhang Y, Feng Q, Wang H, Li G, Jiang W, Li X. Shen H, et al. Gene. 2019 May 25;698:50-60. doi: 10.1016/j.gene.2019.02.044. Epub 2019 Feb 27. Gene. 2019. PMID: 30822475
- GPR56 promotes proliferation of colorectal cancer cells and enhances metastasis via epithelial‑mesenchymal transition through PI3K/AKT signaling activation.Ji B, Feng Y, Sun Y, Ji D, Qian W, Zhang Z, Wang Q, Zhang Y, Zhang C, Sun Y. Ji B, et al. Oncol Rep. 2018 Oct;40(4):1885-1896. doi: 10.3892/or.2018.6582. Epub 2018 Jul 20. Oncol Rep. 2018. PMID: 30066935 Free PMC article.
- Tripartite Motif-Containing Protein 59 (TRIM59) Promotes Epithelial Ovarian Cancer Progression via the Focal Adhesion Kinase(FAK)/AKT/Matrix Metalloproteinase (MMP) Pathway.Zhang P, Zhang H, Wang Y, Zhang P, Qi Y. Zhang P, et al. Med Sci Monit. 2019 May 7;25:3366-3373. doi: 10.12659/MSM.916299. Med Sci Monit. 2019. PMID: 31062766 Free PMC article.
- The role of Tripartite motif containing 59 (TRIM59) in the proliferation and prognosis of intrahepatic cholangiocarcinoma.Zhang JN, Ding DY, Yang SY, Tao QF, Yang Y, Zhou WP. Zhang JN, et al. Pathol Res Pract. 2022 Aug;236:153989. doi: 10.1016/j.prp.2022.153989. Epub 2022 Jun 17. Pathol Res Pract. 2022. PMID: 35753134 Review.
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- Targeting KRAS G12C mutations in colorectal cancer.Zhao MH, Wu AW. Zhao MH, et al. Gastroenterol Rep (Oxf). 2022 Dec 30;11:goac083. doi: 10.1093/gastro/goac083. eCollection 2023. Gastroenterol Rep (Oxf). 2022. PMID: 36632627 Free PMC article. Review.
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- TRIM family contribute to tumorigenesis, cancer development, and drug resistance.Huang N, Sun X, Li P, Liu X, Zhang X, Chen Q, Xin H. Huang N, et al. Exp Hematol Oncol. 2022 Oct 19;11(1):75. doi: 10.1186/s40164-022-00322-w. Exp Hematol Oncol. 2022. PMID: 36261847 Free PMC article. Review.
- The roles and targeting options of TRIM family proteins in tumor.Zhang Y, Zhang W, Zheng L, Guo Q. Zhang Y, et al. Front Pharmacol. 2022 Sep 30;13:999380. doi: 10.3389/fphar.2022.999380. eCollection 2022. Front Pharmacol. 2022. PMID: 36249749 Free PMC article. Review.
- Prognostic Factors of Colorectal Cancer: A Comparative Study on Patients With or Without Liver Metastasis.Peng H, Liu G, Bao Y, Zhang X, Zhou L, Huang C, Song Z, Cao S, Dang S, Zhang J, Huang T, Wu Y, Xu M, Song L, Cao P. Peng H, et al. Front Oncol. 2021 Dec 21;11:626190. doi: 10.3389/fonc.2021.626190. eCollection 2021. Front Oncol. 2021. PMID: 34993129 Free PMC article.
References
-
- Yamada Y, Takayama KI, Fujimura T, Ashikari D, Obinata D, Takahashi S, Ikeda K, Kakutani S, Urano T, Fukuhara H, et al. A novel prognostic factor TRIM44 promotes cell proliferation and migration, and inhibits apoptosis in testicular germ cell tumor. Cancer Sci. 2017;108:32–41. doi: 10.1111/cas.13105. - DOI - PMC - PubMed
Show all 27 references
MeSH terms
- Apoptosis
- Biomarkers, Tumor / metabolism
- Cell Movement*
- Cell Proliferation*
- Colorectal Neoplasms / metabolism
- Colorectal Neoplasms / secondary*
- Epithelial-Mesenchymal Transition
- Female
- Gene Expression Regulation, Neoplastic*
- Humans
- Intracellular Signaling Peptides and Proteins
- Lymphatic Metastasis
- Male
- Membrane Proteins / metabolism*
- Metalloproteins / metabolism*
- Middle Aged
- Neoplasm Invasiveness
- Phosphatidylinositol 3-Kinases / metabolism*
- Prognosis
- Proto-Oncogene Proteins c-akt / metabolism*
- Signal Transduction
- Tripartite Motif Proteins
- Tumor Cells, Cultured
Substances
- Biomarkers, Tumor
- Intracellular Signaling Peptides and Proteins
- Membrane Proteins
- Metalloproteins
- TRIM59 protein, human
- Tripartite Motif Proteins
- Proto-Oncogene Proteins c-akt
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TRIM59 knockdown suppresses the activation of the PI3K/AKT pathways in CRC cells. Expression of PI3K, p-PI3K, AKT, p-AKT was evaluated by western blotting in (A) LoVo and (B) DLD-1 cells. (C) The relative protein expression levels of p-PI3K and p-AKT were quantified in the two cell lines. Each assay is performed in triplicate. Data are presented as mean ± standard deviation; *p Figure 7.
Figure 7.
Overexpression of TRIM59 reduces the…
Figure 7.
Overexpression of TRIM59 reduces the effects of the AKT inhibitor on the migration…
Overexpression of TRIM59 reduces the effects of the AKT inhibitor on the migration and invasion of CRC cells. (A) The levels of TRIM59 mRNA were verified by RT-PCR in the NCM460 cell line after treatment with the TRIM59 plasmid. (B) TRIM59 protein levels were determined using western blotting and quantification is illustrated by a bar chart. (C) Cells were transfected with control plasmid (vehicle) or TRIM59 plasmid and treated with LY294002 (20 µM) for 24 h, following which, migration and invasion assays were performed in these cells. Dimethyl sulfoxide was used as a negative control; **p
All figures (7)
Similar articles
- Knockdown of tripartite motif 59 (TRIM59) inhibits proliferation in cholangiocarcinoma via the PI3K/AKT/mTOR signalling pathway.Shen H, Zhang J, Zhang Y, Feng Q, Wang H, Li G, Jiang W, Li X. Shen H, et al. Gene. 2019 May 25;698:50-60. doi: 10.1016/j.gene.2019.02.044. Epub 2019 Feb 27. Gene. 2019. PMID: 30822475
- GPR56 promotes proliferation of colorectal cancer cells and enhances metastasis via epithelial‑mesenchymal transition through PI3K/AKT signaling activation.Ji B, Feng Y, Sun Y, Ji D, Qian W, Zhang Z, Wang Q, Zhang Y, Zhang C, Sun Y. Ji B, et al. Oncol Rep. 2018 Oct;40(4):1885-1896. doi: 10.3892/or.2018.6582. Epub 2018 Jul 20. Oncol Rep. 2018. PMID: 30066935 Free PMC article.
- Tripartite Motif-Containing Protein 59 (TRIM59) Promotes Epithelial Ovarian Cancer Progression via the Focal Adhesion Kinase(FAK)/AKT/Matrix Metalloproteinase (MMP) Pathway.Zhang P, Zhang H, Wang Y, Zhang P, Qi Y. Zhang P, et al. Med Sci Monit. 2019 May 7;25:3366-3373. doi: 10.12659/MSM.916299. Med Sci Monit. 2019. PMID: 31062766 Free PMC article.
- The role of Tripartite motif containing 59 (TRIM59) in the proliferation and prognosis of intrahepatic cholangiocarcinoma.Zhang JN, Ding DY, Yang SY, Tao QF, Yang Y, Zhou WP. Zhang JN, et al. Pathol Res Pract. 2022 Aug;236:153989. doi: 10.1016/j.prp.2022.153989. Epub 2022 Jun 17. Pathol Res Pract. 2022. PMID: 35753134 Review.
- Multifaceted Roles of TRIM Proteins in Colorectal Carcinoma.Eberhardt W, Haeussler K, Nasrullah U, Pfeilschifter J. Eberhardt W, et al. Int J Mol Sci. 2020 Oct 13;21(20):7532. doi: 10.3390/ijms21207532. Int J Mol Sci. 2020. PMID: 33066016 Free PMC article. Review.
Cited by
- Targeting KRAS G12C mutations in colorectal cancer.Zhao MH, Wu AW. Zhao MH, et al. Gastroenterol Rep (Oxf). 2022 Dec 30;11:goac083. doi: 10.1093/gastro/goac083. eCollection 2023. Gastroenterol Rep (Oxf). 2022. PMID: 36632627 Free PMC article. Review.
- TRIM59 guards ER proteostasis and prevents Bortezomib-mediated colorectal cancer (CRC) cells' killing.Feng X, Yang G, Zhang L, Tao S, Shim JS, Chen L, Wu Q. Feng X, et al. Invest New Drugs. 2022 Dec;40(6):1244-1253. doi: 10.1007/s10637-022-01306-7. Epub 2022 Oct 28. Invest New Drugs. 2022. PMID: 36306030
- TRIM family contribute to tumorigenesis, cancer development, and drug resistance.Huang N, Sun X, Li P, Liu X, Zhang X, Chen Q, Xin H. Huang N, et al. Exp Hematol Oncol. 2022 Oct 19;11(1):75. doi: 10.1186/s40164-022-00322-w. Exp Hematol Oncol. 2022. PMID: 36261847 Free PMC article. Review.
- The roles and targeting options of TRIM family proteins in tumor.Zhang Y, Zhang W, Zheng L, Guo Q. Zhang Y, et al. Front Pharmacol. 2022 Sep 30;13:999380. doi: 10.3389/fphar.2022.999380. eCollection 2022. Front Pharmacol. 2022. PMID: 36249749 Free PMC article. Review.
- Prognostic Factors of Colorectal Cancer: A Comparative Study on Patients With or Without Liver Metastasis.Peng H, Liu G, Bao Y, Zhang X, Zhou L, Huang C, Song Z, Cao S, Dang S, Zhang J, Huang T, Wu Y, Xu M, Song L, Cao P. Peng H, et al. Front Oncol. 2021 Dec 21;11:626190. doi: 10.3389/fonc.2021.626190. eCollection 2021. Front Oncol. 2021. PMID: 34993129 Free PMC article.
References
-
- Yamada Y, Takayama KI, Fujimura T, Ashikari D, Obinata D, Takahashi S, Ikeda K, Kakutani S, Urano T, Fukuhara H, et al. A novel prognostic factor TRIM44 promotes cell proliferation and migration, and inhibits apoptosis in testicular germ cell tumor. Cancer Sci. 2017;108:32–41. doi: 10.1111/cas.13105. - DOI - PMC - PubMed
Show all 27 references
MeSH terms
- Apoptosis
- Biomarkers, Tumor / metabolism
- Cell Movement*
- Cell Proliferation*
- Colorectal Neoplasms / metabolism
- Colorectal Neoplasms / secondary*
- Epithelial-Mesenchymal Transition
- Female
- Gene Expression Regulation, Neoplastic*
- Humans
- Intracellular Signaling Peptides and Proteins
- Lymphatic Metastasis
- Male
- Membrane Proteins / metabolism*
- Metalloproteins / metabolism*
- Middle Aged
- Neoplasm Invasiveness
- Phosphatidylinositol 3-Kinases / metabolism*
- Prognosis
- Proto-Oncogene Proteins c-akt / metabolism*
- Signal Transduction
- Tripartite Motif Proteins
- Tumor Cells, Cultured
Substances
- Biomarkers, Tumor
- Intracellular Signaling Peptides and Proteins
- Membrane Proteins
- Metalloproteins
- TRIM59 protein, human
- Tripartite Motif Proteins
- Proto-Oncogene Proteins c-akt
Related information
LinkOut - more resources
- Full Text Sources
- Other Literature Sources
- Medical
- Research Materials
Full text links [x]
[x]
Cite
Copy Download .nbib
Format: AMA APA MLA NLM
Send To [x]
Overexpression of TRIM59 reduces the effects of the AKT inhibitor on the migration and invasion of CRC cells. (A) The levels of TRIM59 mRNA were verified by RT-PCR in the NCM460 cell line after treatment with the TRIM59 plasmid. (B) TRIM59 protein levels were determined using western blotting and quantification is illustrated by a bar chart. (C) Cells were transfected with control plasmid (vehicle) or TRIM59 plasmid and treated with LY294002 (20 µM) for 24 h, following which, migration and invasion assays were performed in these cells. Dimethyl sulfoxide was used as a negative control; **p
All figures (7)
References
- Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ, He J. Cancer statistics in China, 2015. CA Cancer J Clin. 2016;66:115–132. doi: 10.3322/caac.21338.
- Glockzin G, Schlitt HJ, Piso P. Therapeutic options for peritoneal metastasis arising from colorectal cancer. World J Gastrointest Pharmacol Ther. 2016;7:343–352. doi: 10.4292/wjgpt.v7.i3.343.
- Yamada Y, Takayama KI, Fujimura T, Ashikari D, Obinata D, Takahashi S, Ikeda K, Kakutani S, Urano T, Fukuhara H, et al. A novel prognostic factor TRIM44 promotes cell proliferation and migration, and inhibits apoptosis in testicular germ cell tumor. Cancer Sci. 2017;108:32–41. doi: 10.1111/cas.13105.
- Ozato K, Shin DM, Chang TH, Morse HC., III TRIM family proteins and their emerging roles in innate immunity. Nat Rev Immunol. 2008;8:849–860. doi: 10.1038/nri2413.
- James LC, Keeble AH, Khan Z, Rhodes DA, Trowsdale J. Structural basis for PRYSPRY-mediated tripartite motif (TRIM) protein function. Proc Natl Acad Sci USA. 2007;104:6200–6205. doi: 10.1073/pnas.0609174104.
- Gack MU, Shin YC, Joo CH, Urano T, Liang C, Sun L, Takeuchi O, Akira S, Chen Z, Inoue S, et al. TRIM25 RING-finger E3 ubiquitin ligase is essential for RIG-I-mediated antiviral activity. Nature. 2007;446:916–920. doi: 10.1038/nature05732.
- Khatamianfar V, Valiyeva F, Rennie PS, Lu WY, Yang BB, Bauman GS, Moussa M, Xuan JW. TRIM59, a novel multiple cancer biomarker for immunohistochemical detection of tumorigenesis. BMJ Open. 2012;2:2. doi: 10.1136/bmjopen-2012-001410.
- Nisole S, Stoye JP, Saïb A. TRIM family proteins: Retroviral restriction and antiviral defence. Nat Rev Microbiol. 2005;3:799–808. doi: 10.1038/nrmicro1248.
- Lee OH, Lee J, Lee KH, Woo YM, Kang JH, Yoon HG, Bae SK, Songyang Z, Oh SH, Choi Y. Role of the focal adhesion protein TRIM15 in colon cancer development. Biochim Biophys Acta. 2015;1853:409–421. doi: 10.1016/j.bbamcr.2014.11.007.
- Zhu Z, Wang Y, Zhang C, Yu S, Zhu Q, Hou K, Yan B. TRIM25 blockade by RNA interference inhibited migration and invasion of gastric cancer cells through TGF-β signaling. Sci Rep. 2016;6:19070. doi: 10.1038/srep19070.
- Tan ST, Liu SY, Wu B. TRIM29 overexpression promotes proliferation and survival of bladder cancer cells through NF-κB signaling. Cancer Res Treat. 2016;48:1302–1312. doi: 10.4143/crt.2015.381.
- Zhou Z, Ji Z, Wang Y, Li J, Cao H, Zhu HH, Gao WQ. TRIM59 is up-regulated in gastric tumors, promoting ubiquitination and degradation of p53. Gastroenterology. 2014;147:1043–1054. doi: 10.1053/j.gastro.2014.07.021.
- Zhan W, Han T, Zhang C, Xie C, Gan M, Deng K, Fu M, Wang JB. TRIM59 promotes the proliferation and migration of non-small cell lung cancer cells by upregulating cell cycle related proteins. PLoS One. 2015;10:e0142596. doi: 10.1371/journal.pone.0142596.
- Valiyeva F, Jiang F, Elmaadawi A, Moussa M, Yee SP, Raptis L, Izawa JI, Yang BB, Greenberg NM, Wang F, et al. Characterization of the oncogenic activity of the novel TRIM59 gene in mouse cancer models. Mol Cancer Ther. 2011;10:1229–1240. doi: 10.1158/1535-7163.MCT-11-0077.
- Ma L, Li W. Emodin inhibits LOVO colorectal cancer cell proliferation via the regulation of the Bcl-2/Bax ratio and cytochrome c. Exp Ther Med. 2014;8:1225–1228.
- Zhu W, Ye L, Zhang J, Yu P, Wang H, Ye Z, Tian J. PFK15, a small molecule inhibitor of PFKFB3, induces cell cycle arrest, apoptosis and inhibits invasion in gastric cancer. PLoS One. 2016;11:e0163768. doi: 10.1371/journal.pone.0163768.
- Ye X, Weinberg RA. Epithelial-mesenchymal plasticity: A central regulator of cancer progression. Trends Cell Biol. 2015;25:675–686. doi: 10.1016/j.tcb.2015.07.012.
- Kim JH, Park S, Chung H, Oh S. Wnt5a attenuates the pathogenic effects of the Wnt/β-catenin pathway in human retinal pigment epithelial cells via down-regulating β-catenin and Snail. BMB Rep. 2015;48:525–530. doi: 10.5483/BMBRep.2015.48.9.140.
- Yook JI, Li XY, Ota I, Fearon ER, Weiss SJ. Wnt-dependent regulation of the E-cadherin repressor snail. J Biol Chem. 2005;280:11740–11748. doi: 10.1074/jbc.M413878200.
- Vivanco I, Sawyers CL. The phosphatidylinositol 3-Kinase AKT pathway in human cancer. Nat Rev Cancer. 2002;2:489–501. doi: 10.1038/nrc839.
- Chen Y, Guo Y, Yang H, Shi G, Xu G, Shi J, Yin N, Chen D. TRIM66 overexpresssion contributes to osteosarcoma carcinogenesis and indicates poor survival outcome. Oncotarget. 2015;6:23708–23719. doi: 10.18632/oncotarget.4291.
- Wang J, Zhu J, Dong M, Yu H, Dai X, Li K. Knockdown of tripartite motif containing 24 by lentivirus suppresses cell growth and induces apoptosis in human colorectal cancer cells. Oncol Res. 2014;22:39–45. doi: 10.3727/096504014X14078436005012.
- Liang J, Xing D, Li Z, Shen J, Zhao H, Li S. TRIM59 is upregulated and promotes cell proliferation and migration in human osteosarcoma. Mol Med Rep. 2016;13:5200–5206.
- Aierken G, Seyiti A, Alifu M, Kuerban G. Knockdown of tripartrtite-59 (TRIM59) inhibits cellular proliferation and migration in human cervical cancer cells. Oncol Res. 2017;25:381–388. doi: 10.3727/096504016X14741511303522.
- Townsend AR, Chong LC, Karapetis C, Price TJ. Selective internal radiation therapy for liver metastases from colorectal cancer. Cancer Treat Rev. 2016;50:148–154. doi: 10.1016/j.ctrv.2016.09.007.
- Thiery JP, Acloque H, Huang RY, Nieto MA. Epithelial-mesenchymal transitions in development and disease. Cell. 2009;139:871–890. doi: 10.1016/j.cell.2009.11.007.
- Chen B, Zeng X, He Y, Wang X, Liang Z, Liu J, Zhang P, Zhu H, Xu N, Liang S. STC2 promotes the epithelial-mesenchymal transition of colorectal cancer cells through AKT-ERK signaling pathways. Oncotarget. 2016;7:71400–71416.
Source: PubMed