Poor diagnostic accuracy of commercial antibody-based assays for the diagnosis of acute Chikungunya infection
Stuart D Blacksell, Ampai Tanganuchitcharnchai, Richard G Jarman, Robert V Gibbons, Daniel H Paris, Mark S Bailey, Nicholas P J Day, Ranjan Premaratna, David G Lalloo, H Janaka de Silva, Stuart D Blacksell, Ampai Tanganuchitcharnchai, Richard G Jarman, Robert V Gibbons, Daniel H Paris, Mark S Bailey, Nicholas P J Day, Ranjan Premaratna, David G Lalloo, H Janaka de Silva
Abstract
A Sri Lankan fever cohort (n = 292 patients; 17.8% prevalence) was used to assess two standard diagnostic Chikungunya IgM tests. The immunochromatographic test (ICT) acute sample sensitivity (SN) was 1.9 to 3.9%, and specificity (SP) was 92.5 to 95.0%. The enzyme-linked immunosorbent assay (ELISA) gave an acute sample SN of 3.9% and an SP of 92.5% and a convalescent sample SN of 84% and an SP of 91%. These assays are not suitable for the acute diagnosis of Chikungunya virus infection.
Source: PubMed