The TGR5 gene is expressed in human subcutaneous adipose tissue and is associated with obesity, weight loss and resting metabolic rate

Per-Arne Svensson, Maja Olsson, Johanna C Andersson-Assarsson, Magdalena Taube, Maria J Pereira, Philippe Froguel, Peter Jacobson, Per-Arne Svensson, Maja Olsson, Johanna C Andersson-Assarsson, Magdalena Taube, Maria J Pereira, Philippe Froguel, Peter Jacobson

Abstract

Bile acids have emerged as a new class of signaling molecules that play a role in metabolism. Studies in mice have shown that the bile acid receptor TGR5 mediates several of these effects but the metabolic function of TGR5 in humans is less well established. Here we show that human adipose tissue TGR5 expression is positively correlated to obesity and reduced during diet-induced weight loss. Adipose tissue TGR5 expression was also positively correlated to resting metabolic rate. Our study indicates that human adipose tissue contributes to the TGR5 mediated metabolic effects of bile acids and plays a role in energy expenditure.

Copyright © 2013 Elsevier Inc. All rights reserved.

Figures

Fig. 1
Fig. 1
Adipose tissue TGR5 expression during weight loss. Twenty-eight obese patients (20 women and 8 men) were treated with a very low caloric diet for 12 weeks. Subcutaneous adipose tissue biopsies were obtained by needle aspiration before the diet (day 0, d0) and after 2, 6 and 12 weeks (w) of diet. TGR5 gene expression was analyzed by real-time PCR and normalized to the reference gene LRP10. Data is presented as mean ± SEM. ∗∗p < 0.0005 compared with d0.
Fig. 2
Fig. 2
TGR5 gene expression in human brown and white adipose tissue. (A) TGR5 expression in BAT containing (BAT+) perithyroid adipose tissue and paired subcutaneous white adipose tissue (s.c. WAT, n = 9). (B) TGR5 expression in perirenal adipose tissue containing BAT (BAT+, n = 10) or only containing white adipose tissue (BAT−, n = 10). Data is presented as mean microarray signal ± SEM. n.s. = not significant. Note: the different signal values are due to different microarray types.

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