Optimized [1-(13)C]glucose infusion protocol for 13C magnetic resonance spectroscopy at 3T of human brain glucose metabolism under euglycemic and hypoglycemic conditions
Kim C C van de Ven, Marinette van der Graaf, Cees J J Tack, Dennis W J Klomp, Arend Heerschap, Bastiaan E de Galan, Kim C C van de Ven, Marinette van der Graaf, Cees J J Tack, Dennis W J Klomp, Arend Heerschap, Bastiaan E de Galan
Abstract
The effect of insulin-induced hypoglycemia on cerebral glucose metabolism is largely unknown. (13)C MRS is a unique tool to study cerebral glucose metabolism, but the concurrent requirement for [1-(13)C]glucose administration limits its use under hypoglycemic conditions. To facilitate (13)C MRS data analysis we designed separate [1-(13)C]glucose infusion protocols for hyperinsulinemic euglycemic and hypoglycemic clamps in such a way that plasma isotopic enrichment of glucose was stable and comparable under both glycemic conditions. (13)C MR spectra were acquired with optimized (13)C MRS measurement techniques to obtain high quality (13)C MR spectra with these protocols.
Conflict of interest statement
Disclosure/conflict of interest
The authors have no conflicts of interest to disclose relevant to this paper.
(c) 2009 Elsevier B.V. All rights reserved.
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Source: PubMed