Correlation of CD33 expression level with disease characteristics and response to gemtuzumab ozogamicin containing chemotherapy in childhood AML

Abstract

CD33 is expressed on the majority of acute myeloid leukemia (AML) leukemic blasts and is the target for gemtuzumab ozogamicin (GO), a toxin-conjugated anti-CD33 mAb. In the present study, we quantified the CD33 mean fluorescent intensity of leukemic blasts prospectively in 619 de novo pediatric AML patients enrolled in Children's Oncology Group GO-containing clinical trials and determined its correlation with disease characteristics and clinical outcome. CD33 expression varied more than 2-log fold; a median mean fluorescent intensity of 129 (range, 3-1550.07) was observed. Patients were divided into 4 quartiles, quartiles 1-4 (Q1-4) based on expression and disease characteristics and clinical response defined across quartiles. High CD33 expression was associated with high-risk FLT3/ITD mutations (P < .001) and was inversely associated with low-risk disease (P < .001). Complete remission (CR) rates were similar, but patients in Q4 had significantly lower overall survival (57% ± 16% vs 77% ± 7%, P = .002) and disease-free survival from CR (44% ± 16% vs 62% ± 8%, P = .022). In a multivariate model, high CD33 expression remained a significant predictor of overall survival (P = .011) and disease-free survival (P = .038) from CR. Our findings suggest that CD33 expression is heterogeneous within de novo pediatric AML. High expression is associated with adverse disease features and is an independent predictor of inferior outcome. The correlation between CD33 expression and GO response is under investigation. These studies are registered at www.clinicaltrials.gov as NCT00070174 and NCT00372593.

Figures

Figure 1

Distribution of CD33 expression. (A) Distribution of CD33 expression for the 619 participants in our study cohort. (B) Correlation of CD33 expression level with specific cytogenetic/molecular disease characteristics by quartile. (C) CD33 expression and association with disease risk-group classification by quartile.

Figure 2

Correlation of clinical outcome with CD33 expression quartile. (A) OS from CR for Q1-3 versus Q4 for all AAML03P1 patients. (B) DFS from CR for Q1-3 versus Q4 for all patients enrolled in AAML03P1.

Figure 3

Correlation of clinical outcome with CD33 expression by cytogenetic/molecular risk classification. Shown is the OS from CR based on CD33 expression for patients with low-risk (A), high-risk (B), and standard-risk disease (C).