Preclinical pharmacokinetics of radiolabelled hyaluronan
Milan Laznicek, Alice Laznickova, Dagmar Cozikova, Vladimir Velebny, Milan Laznicek, Alice Laznickova, Dagmar Cozikova, Vladimir Velebny
Abstract
Background: Hyaluronan (HYA) is a high molecular weight glucosaminoglycan with a great perspective for medical applications. Because HYA is widespread in the body, it is difficult to determine the fate of exogenously administered HYA.
Methods: In this study, HYAof different molecular weights (0.1-1 MDa) was labelled with (99m)Tc, and the distribution profiles were determined after administrating the HYA to rats.
Results: After the intravenous administration of (99m)Tc-HYA, a rapid decrease in the radioactivity of blood samples was observed, presumably because of (99m)Tc-HYA uptake by the liver; only minimal signs of liver radioactivity washout were detected. After the oral administration of (99m)Tc-HYA, no significant absorption to the central compartment was found. A preliminary study using (14)C-HYA exhibited a different distribution profile than (99m)Tc-HYA because of the different administered dose and the fate of the degradation products. Even with (14)C-HYA, only traces of radioactivity were absorbed after oral administration.
Conclusion: This paper provides quantitative information regarding the distribution parameters of radiolabelled HYA in preclinical experiments.
Source: PubMed