COLAR: open-label clinical study of IL-6 blockade with tocilizumab for the treatment of immune checkpoint inhibitor-induced colitis and arthritis

Abstract

Background: Immune-related adverse events due to immune checkpoint inhibitors (ICIs) are not always effectively treated using glucocorticoids and it may negatively affect the antitumor efficacy of ICIs. Interventional studies of alternatives to glucocorticoids are lacking. We examined whether interleukin-6 blockade by tocilizumab reduced ICI-induced colitis and arthritis.

Patients and methods: Patients with solid cancer experiencing Common Terminology Criteria for Adverse Events (CTCAE v5.0) grade >1 ICI-induced colitis/diarrhea (n=9), arthritis (n=9), or both (n=2) were recruited and treated with tocilizumab (8 mg/kg) every 4 weeks until worsening or unacceptable toxicity. Patients were not allowed to receive systemic glucocorticoids and other immunosuppressive drugs within the 14-day screening period. The primary endpoint was clinical improvement of colitis and arthritis, defined as ≥1 grade CTCAE reduction within 8 weeks. Secondary endpoints were improvements and glucocorticoid-free remission at week 24; safety; radiologic, endoscopic, and histological changes; and changes in plasma concentrations of C reactive protein, cytokines (IL-6, IL-8, and IL-17), and YKL-40.

Results: Nineteen patients were available for efficacy analysis; one patient was excluded due to pancreatic insufficiency-induced diarrhea. Patients received treatment with pembrolizumab (n=10) or nivolumab (n=4) as monotherapy or ipilimumab and nivolumab (n=5) combined. Seven patients had been initially treated with glucocorticoids, and two of them also received infliximab. Ten patients continued ICI therapy during tocilizumab treatment. The primary endpoint was achieved in 15 of 19 (79%) patients. Additional one patient had ≥1 grade reduction at week 10, and another patient had stabilized symptoms. At week 24, ongoing improvement without glucocorticoids (n=12), including complete remission (n=10), was noted. Five patients had grades 3-4 treatment-related adverse events, which were manageable and reversible.

Conclusions: Tocilizumab showed promising clinical efficacy and a manageable safety profile in the treatment of ICI-induced colitis and arthritis. Our findings support the feasibility of randomized trials of immune-related adverse events.

Trial registration number: NCT03601611.

Keywords: CTLA-4 Antigen; Cytokines; Cytotoxicity, Immunologic; Immunotherapy; Programmed Cell Death 1 Receptor.

Conflict of interest statement

Competing interests: All authors completed and submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. RBH received speaking honoraria from BPNO. P. Vilmann reported a consulting relationship with MediGlobe GmbH, Grassau. JJ reported receiving research funding and hotel/airfare reimbursement to attend global health meetings from Roche, BMS, and Celgene. MKB reported receiving hotel/airfare reimbursement to attend global health meetings from Pfizer. RHLE received research funding from BMS and advisory relationship with Amgen. IMS has been principal investigator in BMS-supported trials and received honoraria from BMS for speaking and serving on an advisory board. IMC reported receiving research funding and hotel/airfare reimbursement to attend global health meetings from Roche, BMS, Celgene, Genis, and advisory relationship with Amgen. OHN, LBR, ST, SJ, and DLN had no disclosures.

© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

Figures

Figure 1

Treatment overview. All 20 patients are illustrated. Patient C8 was excluded from efficacy analysis due to pancreatic insufficiency-induced diarrhea. Treatment for ICIs and tocilizumab are shown from the time point of tocilizumab initiation. Nine of 20 patients received systematic therapy with systemic glucocorticoids. Six patients experienced cancer progression within the study period (24 weeks), including C18 with melanoma, who had new melanoma moles which were surgically resected and followed by a durable complete response. At the cut-off for disease status in October 2021, three additional patients experienced cancer progression; two were rechallenged with a PD-1 inhibitor. ICI, immune checkpoint inhibitor; NE, not evaluable; PD, progressive disease; PFS, progression-free survival; TCZ, tocilizumab.

Figure 2

Plasma concentrations of IL-6, IL-8, IL-17, YKL-40, and C reactive protein (CRP). (A–E) Plasma concentrations of IL-6, IL-8, IL-17, YKL-40, and CRP during the first 4 weeks of tocilizumab among responders and non-responders. (F–J) Plasma concentrations of IL-6, IL-8, IL-17, YKL-40, and CRP in responding patients. IL, interleukin; YKL-40, chitinase-3-like-protein 1.