HIV DNA loads, plasma residual viraemia and risk of virological rebound in heavily treated, virologically suppressed HIV-infected patients
N Gianotti, F Canducci, L Galli, F Cossarini, S Salpietro, A Poli, S Nozza, V Spagnuolo, M Clementi, M Sampaolo, E R Ceresola, S Racca, A Lazzarin, A Castagna, N Gianotti, F Canducci, L Galli, F Cossarini, S Salpietro, A Poli, S Nozza, V Spagnuolo, M Clementi, M Sampaolo, E R Ceresola, S Racca, A Lazzarin, A Castagna
Abstract
In this single-centre, retrospective study, we analyzed data of 194 patients receiving antiretroviral therapy with <50 human immunodeficiency virus (HIV) RNA copies/mL in plasma and 318 HIV RNA/DNA paired samples. By kinetic polymerase chain reaction (kPCR) molecular system analysis, 104 (54%) subjects had undetectable HIV RNA and 90 (46%) had residual viraemia. Median (interquartile range) HIV DNA load was 780 (380-1930) copies/10(6) peripheral blood lymphocytes (PBL), and HIV DNA loads were independently associated with residual viraemia (p 0.002). Virological rebound occurred in 29/194 (15%) patients over a median (interquartile range) follow-up of 17.5 (13.5-31.5) months. Residual viraemia (p 0.002), but not HIV DNA load, was independently associated with virological rebound.
Keywords: HIV-1 DNA load; kPCR; residual viraemia; virological failure; virological rebound.
Copyright © 2014 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
Source: PubMed