Antioxidant and Anti-Inflammatory Effect of Probiotic Lactobacillus plantarum KU15149 Derived from Korean Homemade Diced-Radish Kimchi

Kyoung Jun Han, Ji-Eun Lee, Na-Kyoung Lee, Hyun-Dong Paik, Kyoung Jun Han, Ji-Eun Lee, Na-Kyoung Lee, Hyun-Dong Paik

Abstract

Lactobacillus plantarum KU15149 was demonstrated to have probiotic behavior and functions, including antioxidant and anti-inflammatory activity. L. plantarum KU15149 obtained from homemade diced-radish kimchi has a high survival rate under artificial gastric acid (pH 2.5, 0.3% pepsin) and bile salt (0.3% oxgall) conditions. However, L. plantarum KU15149 did not produce β-glucuronidase, which is known to be a carcinogenic enzyme with resistance to several antibiotics, such as gentamycin, kanamycin, streptomycin, tetracycline, and ciprofloxacin. L. plantarum KU15149 strongly adhered to HT-29 cells and had high antioxidant activity in terms of 2,2-diphenyl- 1-picrylhydrazyl (DPPH) free radical-scavenging and β-carotene bleaching assays. L. plantarum KU15149 also exhibited a pronounced inhibition of nitric oxide (NO) production, along with expression of nitric oxide synthase (iNOS) and cyclooxygenase -2 (COX-2) as well as proinflammatory cytokines, such as TNF-α, IL-1β, and IL-6, when RAW 264.7 cells were stimulated with LPS. Therefore, L. plantarum KU15149 exhibited pharmaceutical functionality as a potential probiotic.

Keywords: Lactobacillus plantarum; Probiotics; anti-inflammatory; antioxidant; kimchi.

Conflict of interest statement

Conflict of Interest

The authors have no financial conflicts of interest to declare.

Figures

Fig. 1. Adhesion of LAB strains to…
Fig. 1. Adhesion of LAB strains to HT-29 cells.
LGG, L. rhamnosus GG; KU15149, L. plantarum KU15149; KU15176, L. brevis KU15176. Error bars indicate standard deviation of three independent experiments. Letters indicate a significant difference between prospective and commercial probiotic strain. All values are expressed as the mean ± standard deviation. Values with different letters indicate significant differences for each characteristic (p < 0.05).
Fig. 2. Antioxidant activity of LAB strains…
Fig. 2. Antioxidant activity of LAB strains assessed by (A) DPPH free-radical scavenging and (B) β-carotene bleaching assays.
BHT, Butylated hydroxytoluene; LGG, L. rhamnosus GG; KU15149, L. plantarum KU15149; KU15176, L. brevis KU15176. Letters a–c denote a significant difference between prospective and commercial probiotic strains. Each value is expressed as the mean ± standard deviation. The different letters on the error bars represent statistically significant differences between values (p < 0.05).
Fig. 3. Production of (A) NO on…
Fig. 3. Production of (A) NO on LAB strains in lipopolysaccharide (LPS)-stimulated RAW264.7 cells and the relative expression of mRNA level of (B) TNF-α, (C) iNOS, (D) COX-2, (E) IL-1β, and (F) IL-6. LPS-, without LPS treatment; LPS+, treated with LPS (1 µg/ml); LGG, L. rhamnosus GG with LPS; KU15149, L. plantarum KU15149 with LPS.
All values are expressed as the mean ± standard deviation and standardized against the β-actin housekeeping gene. Values with different letters indicate significant differences for each characteristic (p < 0.05).

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