Protective Effect of Exercise on Acute High-fat Overfeeding (HFDEXC)

Inclusion Criteria:

• Males or females between the ages of 20 and 40 years
• A body mass index (BMI) of 20.0 - 29.9 kg/m2.
• Low risk for risk of injury or health complications as determined by physical activity readiness questionnaire (PARQ).

Exclusion Criteria:

• Are currently taking any antioxidant supplementation (daily multivitamin not included), prescribed non-steroidal anti-inflammatory drugs (NSAIDS), antibiotics, steroids, or probiotics.
• Are currently taking any medications for management of cholesterol, diabetes, or body weight/obesity, or are undergoing hormone therapy.
• Are unwilling or unable to comply with study procedures, at the discretion of the investigators.
• Are unwilling to have biospecimens (blood and urine) collected and stored (only blood) for future research.
• Pregnant women - A urine pregnancy test will be conducted prior to any testing procedures.
• A history of disordered eating, including anorexia nervosa, bulimia nervosa, binge eating disorder, or avoidant restrictive food intake disorder or deemed high-risk for disordered eating via questionnaire.
• A history of gastrointestinal diseases, such as galactosemia, gastroesophageal reflux disease (GERD), irritable bowel syndrome (IBS), Celiac disease, Crohn's disease, or lactose intolerance.
• Individuals with known allergies to wheat or gluten, milk, soy, tree nuts (e.g., almonds, walnuts, pecans), or peanuts.
• Deemed high risk for food insecurity via the Hunger Vital Sign screener.
• Have followed a ketogenic diet within the last three months or have been told to avoid a high-fat diet by a medical provider for risk of cardiovascular disease.
• Cardiorespiratory fitness (VO2max) below the 30th percentile for age and sex (measured at initial screening visit)