Effect of a daily dose of Lactobacillus brevis CD2 lozenges in high caries risk schoolchildren

Guglielmo Campus, Fabio Cocco, Giovanna Carta, Maria Grazia Cagetti, Charlotte Simark-Mattson, Laura Strohmenger, Peter Lingström, Guglielmo Campus, Fabio Cocco, Giovanna Carta, Maria Grazia Cagetti, Charlotte Simark-Mattson, Laura Strohmenger, Peter Lingström

Abstract

Objectives: A double-blind, randomised, placebo-controlled clinical trial was performed to validate the hypothesis that the use of lozenges containing Lactobacillus brevis CD2 (Inersan®, CD Investments srl) may reduce plaque pH, salivary mutans streptococci (ms) and bleeding on probing, during a 6-week period, in a sample of high caries risk schoolchildren.

Methods: A total of 191 children (aged 6-8 years), presenting two to three carious lesions and a salivary ms concentration of ≥10(5) CFU/ml, were enrolled and divided into two groups, an L. brevis CD2 lozenge group and a no L. brevis lozenge group, and examined at baseline (t0), after 3 weeks (t1), after 6 weeks of lozenge use (t2) and 2 weeks after the cessation of lozenge use (t3). Plaque pH was assessed using the microtouch technique following a sucrose challenge. The area under the curve (AUC5.7 and AUC6.2) was recorded. Salivary ms were counted, and bleeding on probing was assessed.

Results: At t0, the plaque-pH and ms concentration values were similar in both groups. Mean areas (AUC5.7 and AUC6.2) were significantly greater in the control group at t1, t2 and t3. L. brevis CD2 lozenges significantly reduced salivary ms concentrations and bleeding. The subjects from the test group showed a statistically significant decrease (p = 0.01) in salivary ms concentration. At t2, a statistically significantly lower bleeding value was recorded in the test group compared with the control group (p = 0.02).

Conclusions: Six weeks' use of lozenges containing L. brevis CD2 had a beneficial effect on some important variables related to oral health, including a reduction in plaque acidogenicity, salivary ms and bleeding on probing. (Trial Registration Number NCT01601145 08/21/2012).

Figures

Fig. 1
Fig. 1
Flow chart of the study design
Fig. 2
Fig. 2
Bleeding score (percentage) recorded at t0, t2 and t3 in the two groups of children using lozenge with L. brevis CD2 respective lozenge without probiotic bacteria (control). One-way ANOVA

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