Viral etiologies of acute respiratory infections among hospitalized Vietnamese children in Ho Chi Minh City, 2004-2008

Anh Ha Lien Do, H Rogier van Doorn, My Ngoc Nghiem, Juliet E Bryant, Thanh Hang thi Hoang, Quang Ha Do, Tan Le Van, Tan Thanh Tran, Bridget Wills, Vinh Chau van Nguyen, Minh Hien Vo, Cong Khanh Vo, Minh Dung Nguyen, Jeremy Farrar, Tinh Hien Tran, Menno D de Jong, Anh Ha Lien Do, H Rogier van Doorn, My Ngoc Nghiem, Juliet E Bryant, Thanh Hang thi Hoang, Quang Ha Do, Tan Le Van, Tan Thanh Tran, Bridget Wills, Vinh Chau van Nguyen, Minh Hien Vo, Cong Khanh Vo, Minh Dung Nguyen, Jeremy Farrar, Tinh Hien Tran, Menno D de Jong

Abstract

Background: The dominant viral etiologies responsible for acute respiratory infections (ARIs) are poorly understood, particularly among hospitalized children in resource-limited tropical countries where morbidity and mortality caused by ARIs are highest. Improved etiological insight is needed to improve clinical management and prevention.

Objectives: We conducted a three-year prospective descriptive study of severe respiratory illness among children from 2 months to 13 years of age within the largest referral hospital for infectious diseases in southern Vietnam.

Methods: Molecular detection for 15 viral species and subtypes was performed on three types of respiratory specimens (nose, throat swabs and nasopharyngeal aspirates) using a multiplex RT-PCR kit (Seeplex™ RV detection, Seegene) and additional monoplex real-time RT-PCRs.

Results: A total of 309 children were enrolled from November 2004 to January 2008. Viruses were identified in 72% (222/309) of cases, including respiratory syncytial virus (24%), influenza virus A and B (17%), human bocavirus (16%), enterovirus (9%), human coronavirus (8%), human metapneumovirus (7%), parainfluenza virus 1-3 (6%), adenovirus (5%), and human rhinovirus A (4%). Co-infections with multiple viruses were detected in 20% (62/309) of patients. When combined, diagnostic yields in nose and throat swabs were similar to nasopharyngeal aspirates.

Conclusion: Similar to other parts in the world, RSV and influenza were the predominant viral pathogens detected in Vietnamese hospitalized children. Combined nasal and throat swabs are the specimens of choice for sensitive molecular detection of a broad panel of viral agents. Further research is required to better understand the clinical significance of single versus multiple viral coinfections and to address the role of bacterial (co-)infections involved in severe respiratory illness.

Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1. Proportion of RSV and hMPV…
Figure 1. Proportion of RSV and hMPV positive cases recruited each month from November 2004 to January 2008.
Time in months is displayed on the X-axis and the percentage of positive cases of each virus among all cases recruited in that month on the y-axis.
Figure 2. Number of cases enrolled and…
Figure 2. Number of cases enrolled and total numbers of ARI children hospitalized in HTD, November 2004 to January 2008.

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Source: PubMed

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