Adipose Tissue and Inflammation in Coronary Heart Disease (ATICH)
Inflammatory Activity in Various Compartments of Adipose Tissue in Patients With Coronary Heart Disease
研究概览
地位
条件
详细说明
The present study is observational with biochemical end-points to disclose inflammatory mechanisms in various compartments of adipose tissue in patients with coronary heart disease (CHD) compared to patients without significant CHD. Fifty patients with CHD undergoing coronary artery bypass surgery with open chest procedure will be included and 20 patients with valve disease without overt CHD undergoing open valve surgery will serve as a control group. No specific restrictions to inclusion other than lack of consent to participate after written and oral information are listed. In patients undergoing open heart surgery for CHD, representative peri-operative biopsies from epicardial, pericardial and subcutaneous fat will be gathered, carefully processed and deep-frozen to 80 degrees of frost for later analyses on histological/cellular components and on messenger ribonucleic acid for selected inflammatory variables. Simultaneously, blood samples (arterial blood at start of operation) will be collected, processed and deep-frozen at 80 degrees of frost for later analyses of relevant circulating inflammatory variables to correlation analyses with tissue samples, possibly also PaxGene tubes for messenger ribonucleic acid in circulating leukocytes.
The study will be conducted according to the Declaration of Helsinki, and in accordance with approval from the Regional Ethics Committee.
研究类型
注册 (预期的)
联系人和位置
学习地点
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Oslo、挪威、0407
- Svein Solheim
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参与标准
资格标准
适合学习的年龄
接受健康志愿者
有资格学习的性别
取样方法
研究人群
描述
Inclusion Criteria:
- Patients with coronary heart disease undergoing coronary artery bypass surgery
Exclusion Criteria:
- None
学习计划
研究是如何设计的?
设计细节
队列和干预
团体/队列 |
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Coronary heart disease
Patients with coronary heart disease undergoing planned coronary artery bypass surgery
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研究衡量的是什么?
主要结果指标
结果测量 |
措施说明 |
大体时间 |
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Inflammasome-related proteins
大体时间:At inclusion
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Inflammasome-related proteins (IL-18, IL-1b, NLRP3, Caspase, TLR4, IL-6, IL-6R, gp130), markers of remodeling (i.e.
MMP-9, TIMP-1, PAI-1, Galectin, EMMPRIN), adipokines (i.e adiponectin, visfatin), other relevant pro-and anti-inflammatory cytokines (like TNF-alpha, IL-8, IL-12, IFN-gamma, MCP-1, IL-4, IL-10, TGF beta.)
Relevant laboratory methods; qPCR (mRNA - all compartments + PAXGenetubes (leukocytes); Immunohistochemically; Circulating (where possible) (ELISAs and/or Multiplex).
Results will be recorded in a database for further statistical analyses.
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At inclusion
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合作者和调查者
调查人员
- 首席研究员:Ingebjørg Seljeflot, Professor、Oslo University Hospital, Ullevål, Oslo, Norway
研究记录日期
研究主要日期
学习开始 (实际的)
初级完成 (预期的)
研究完成 (预期的)
研究注册日期
首次提交
首先提交符合 QC 标准的
首次发布 (估计)
研究记录更新
最后更新发布 (实际的)
上次提交的符合 QC 标准的更新
最后验证
更多信息
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