- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT00154518
The Role of Matrix Metalloproteinases (MMPs) in Orthodontic Tooth Movement
The Role of Matrix Metalloproteinases in Orthodontic Treatment
Study Overview
Status
Conditions
Intervention / Treatment
Detailed Description
Long treatment time is a major factor causing high fees for orthodontic treatment. Patients would have dental caries or periodontitis resulting from improper oral hygiene care during this long treatment period. How to speed up the tooth movement which determines the duration of orthodontic treatment, can help more people to obtain good occlusion and esthetics. Orthodontic force on a tooth induces bone resorption on the compression side and bone deposition on the tension side, thus the bone remodels and then the tooth moves. Therefore, bone resorption is the rate-limiting step of a lengthy orthodontic treatment.
Bone resorption is a complex process. The mineral component is dissolved by acid from osteoclasts. On the other hand, the organic components are digested with proteolytic enzymes secreted from osteoblasts and osteoclasts. We focus on our study on specific proteases which can digest extracellular matrix, called matrix metalloproteinases (MMPs). Osteoblast-derived MMPs play an important role during initiation of bone resorption. However, the mechanism of its regulation is not clear. The past studies applied stretching or tension on single layer of cultured cells to characterize cellular response to the mechanical stimulation. Now we simulate part of the bone resorption process by cultivating osteocyte-like cells in three-dimensional collagen gel under periodical compression.
In a preliminary study, we focus on transcriptional changes of MMPs upon compression in an osteosarcoma cell line MG-63. Initial data form microarray indicated specific increase of two MMPs expression after one day of compression. This increased expression was specific because the levels of house-keeping genes (ex. Beta-actin or GAPDH) and bone-specific markers were unaltered. Therefore, we proposed that increased MMP expression of osteoblasts under compression is the first step for bone remodeling switching from synthesis to degradation of osteoid. In order to test this hypothesis, the following specific aims will be achieved:
- To test whether these two MMPs can be up-regulated during orthodontic treatment. Alveolar bone samples will be collected from partially impacted third molars after orthodontic uprighting for different periods of time in volunteers. In situ hybridization and immunohistochemistry analysis for MMPs will reveal their roles in this physiological process.
- To optimize the regulation by changing the magnitude and frequency of the pressure, and characterize the time table for these changes.
Study Type
Enrollment (Anticipated)
Phase
- Phase 1
Contacts and Locations
Study Locations
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Taipei, Taiwan
- Department of Dentistry, National Taiwan University Hospital
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Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Genders Eligible for Study
Description
Inclusion Criteria:
- Two mandibular third molars containing at least one mesially angular impaction with crown exposed to oral cavity
Exclusion Criteria:
- Pregnancy
Study Plan
How is the study designed?
Design Details
- Primary Purpose: Treatment
- Allocation: Non-Randomized
- Interventional Model: Factorial Assignment
- Masking: Single
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
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Tissue staining with antibody
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Collaborators and Investigators
Investigators
- Principal Investigator: Chung-Chen Yao, DDS, PhD, National Taiwan University Hospital
Study record dates
Study Major Dates
Study Start
Study Completion
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Estimate)
Study Record Updates
Last Update Posted (Estimate)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Additional Relevant MeSH Terms
Other Study ID Numbers
- 9261701411
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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